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Articles by Ping Hu
Total Records ( 6 ) for Ping Hu
  Kimberlee A. West , David R. Johnson , Ping Hu , Todd Z. DeSantis , Eoin L. Brodie , Patrick K. H. Lee , Helene Feil , Gary L. Andersen , Stephen H. Zinder and Lisa Alvarez-Cohen
  Tetrachloroethene (PCE) and trichloroethene (TCE) are prevalent groundwater contaminants that can be completely reductively dehalogenated by some "Dehalococcoides" organisms. A Dehalococcoides-organism-containing microbial consortium (referred to as ANAS) with the ability to degrade TCE to ethene, an innocuous end product, was previously enriched from contaminated soil. A whole-genome photolithographic microarray was developed based on the genome of "Dehalococcoides ethenogenes" 195. This microarray contains probes designed to hybridize to >99% of the predicted protein-coding sequences in the strain 195 genome. DNA from ANAS was hybridized to the microarray to characterize the genomic content of the ANAS enrichment. The microarray results revealed that the genes associated with central metabolism, including an apparently incomplete carbon fixation pathway, cobalamin-salvaging system, nitrogen fixation pathway, and five hydrogenase complexes, are present in both strain 195 and ANAS. Although the gene encoding the TCE reductase, tceA, was detected, 13 of the 19 reductive dehalogenase genes present in strain 195 were not detected in ANAS. Additionally, 88% of the genes in predicted integrated genetic elements in strain 195 were not detected in ANAS, consistent with these elements being genetically mobile. Sections of the tryptophan operon and an operon encoding an ABC transporter in strain 195 were also not detected in ANAS. These insights into the diversity of Dehalococcoides genomes will improve our understanding of the physiology and evolution of these bacteria, which is essential in developing effective strategies for the bioremediation of PCE and TCE in the environment.
  Ping Hu and Zongyao Wang
  In this study, we aim to put forward a novel nonmonotone algorithm of moving asymptotes for solving n-variate unconstrained optimization problems. The algorithm first generates n separable subproblems by virtue of the moving asymptotes function in each iteration to determine the descent search direction and then obtain the step by new nonmonotone line search techniques. The global convergence of the proposed algorithm is established in this study. In addition, we give some numerical tests, from which it is indicates that the new algorithm is effective in solving multi-peak or large-scale optimization problems.
  Li-Li Li , Ping Hu , Bi-Qin Wang , Wen-Hao Yu , Yo Shimizu and Ke-Qing Zhao
  Truxene discotic liquid crystals are interesting for their polymesomorphism and as organic semiconductor candidates. We developed an efficient synthetic method for C3-symmetrical truxene derivative 2,7,12-trihydroxy-3,8,13-trimethoxytruxene 5, and further a series of ether-ester mixed tail truxene discogens HTn, TR(OMe)3[OOCC6H2(OCnH2n+1)3]3 were prepared. The truxene discogens HTn (n = 4-8, 10, 12) were characterised by using 1H and 13C nuclear magnetic resonance, and high-resolution mass spectrometry. Their thermal stability was studied with thermal gravimetric analysis and their liquid crystal properties were investigated by using polarising optical microscopy, differential scanning calorimetry and X-ray diffraction. They exhibit a hexagonal columnar (Colh) mesophase in fairly wide temperature ranges.
  Dejiang Chen , Weijie Li , Xinghong Zhang , Ping Hu , Jiecai Han , Changqing Hong and Wenbo Han
  This paper investigated the thermal shock behavior of hot-pressed zirconium diboride (ZrB2–10 vol%SiC–10 vol%ZrO2) composite by means of water quenching. Under single thermal shock, the retained strength presented sharp degradation with the temperature difference (ΔT) above 500 °C. Differently, the retained strength decreased rapidly with ΔT above 300 °C under five-cycle thermal shock. Analysis of the microstructural features also showed discrepancy for the composite under different thermal shock conditions. Further discussion found that the thermal shock resistance of ZrB2–SiC–ZrO2 composite was influenced by the intrinsic properties as well as the oxidation behavior under high temperature.
  Tao Zhu , Weijie Li , Xinghong Zhang , Ping Hu , Changqing Hong and Ling Weng
  ZrB2–10 vol.% SiC ceramics were hot pressed with addition of two contents of ZrO2, i.e., 10 vol.% and 20 vol.%. Oxidation behavior of ZrB2–SiC–ZrO2 ceramics was investigated using muffle furnace in the temperature range of 800–1200 °C in air. With the increase in temperature and extension in holding time, two ceramics both presented more and more severe oxidation behavior. Comparatively, results of microstructure indicated that ceramic with 10 vol.% ZrO2 always exhibited lighter variation in structure after oxidation. Further discussion showed that ceramic with 10 vol.% ZrO2 also provided lower mass gain and thinner oxidized layer. Analysis of oxidation mechanism revealed that excessive addition of ZrO2 brought much path to the inward transport of oxygen and therefore led to the serious oxidation for ZrB2–SiC–ZrO2 ceramics.
  Xiangying Sun , Bin Liu , Ping Hu and Wenying Hu
  Fluorescent dye Acridine Orange (AO) was indirectly assembled onto the surface of colloidal gold nanoparticles modified quartz and gold wafer, with cysteine as mediation. Strong fluorescence emission was observed from AO self-assembled multilayers. Formation of the self-assembled multilayers was confirmed and primarily characterized by electrochemical and fluorescence methods. The effect of assembling substrate on fluorescence intensity of the film was discussed. The self-assembled multilayers offer a high sensitive interfacial fluorescence sensing of deoxyribonucleic acid with the detection limit of 7.145 x 10- 9 g L- 1 (3 S.D./K), which was five orders of magnitude lower than that of the bulk solution-phase. The self-assembled multilayers film has higher stability and good regeneration capacity.
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