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Articles by P.K. Das
Total Records ( 4 ) for P.K. Das
  S. Gantait , N. Mandal and P.K. Das
  The genus Allium, consists of hundreds of medicinal plant species, is one of the most imperative sources of life supporting drugs. The in vitro biotechnological interventions are vital to choose, multiply, store up and improve the major Allium sp. In vitro culture of Allium has performed an incredibly crucial role in accelerated growth of several species with desirable traits and production of healthy and disinfectant propagules as well as paved the way towards cultivar improvement. During the last quite a few years, several moves have been made for in vitro propagation of Allium. In vitro regeneration via direct and indirect organogenesis using different explants and plant growth regulator formulations has been comprehensively covered in the literature. Recent challenges for establishment of protocols for genetic transformation have gained preference in the recent past reports. This review article comprehensively describes the exploitation of biotechnology for in vitro regeneration and genetic transformation for enhancement of the genus Allium.
  K. Das , P. Asaithambi , S. Sanyal , P.K. Das , P.R. Ghosh and A. Chakrabarti
  To ascertain the physiological functions of the adrenal glands, single stage bilateral adrenalectomy was performed on six female Bengal goats (Capra hircus). Blood and urine samples were collected at regular interval in post-operative periods for estimation of different parameters. A radioimmunoassay detected an invariable drop in plasma total cortisol and also a downward course with erratic nature of the flurometrically assessed urinary total catecholamines represent the adrenal insufficiency among these animals. A steady post-operative weight loss was evident in all of the adrenalectomized goats. Other progressive symptoms like anorexia, asphyxia, muscular asthenia were profound like those which occur in other mammalian species. Increased melanogenesis was established by the urinalysis. Long duration surviving goats with adrenal insufficiency showed a marked discolouration and pigmentation of the skin. The average survival period for the adrenalectomized goats reported in this study was 168 h. The influence of certain factors obtaining in the experimental situation upon survival is discussed in some detail. The goat may survive in some instances and it might be an exception to the concept that adrenalectomy is fatal to the mammals.
  S. Gantait , N. Mandal , S. Bhattacharyya and P.K. Das
  Aloe (Aloe vera L., Family Liliaceae), with proven multiple medicinal values finds favour readily in Ayurevdic applications, is facing a serious threat to its population as well as biodiversity due to its popularly harvested aloe leaves by the local communities and herbal medicine vendors. To succeed in dealing with the specified problem along with the facilitation of the mass production of commercial level, constant supply of quality propagules can be possible through conservation of propagules in vitro. The present study is thus concerned with in vitro conservation of multiple shoot culture of aloe to achieve unbroken supply of propagules maintaining their genetic purity. Rhizomatous stem explants result multiple bud break in MS plus 0.25 mg L-1 of NAA and 1.5 mg L-1 of BAP. Separated shoot buds further result in shoot multiplication and proliferation on MS with 2.5 mg L-1 of BAP. In vitro generated multiple shoots were split into individual shoot and subcultured for further multiplication in a sustainable. Five subcultures were performed at an extended 5 month interval over a period of 25 months in the same medium. Plantlets regenerated after 1st subculture and plantlets from 5th subculture showed no significant difference in the phenotypic response. Genetic integrity of in vitro clones was tested using ISSR primers. All monomorphic bands in the ISSR assay, both for primary culture as well long term culture, ascertained their genetic integrity to a great extent.
  P.K. Das , P.R. Ghosh , B. Roy and D. Mazumdar
  Family poultry is presently considered as a Special Programme for Food Security particularly at the developing countries in the world. In this outset, a study was conducted on 60000 Rhode Island Red (RIR) birds by twenty four major treatment types to identify the best combination of major inputs viz. age of birds, initial supply of concentrated feed upto grower stage followed by supplementation of ration produced by the farmers using locally available ingredients along with run space to develop a model on backyard poultry production system under scavenging at five agroclimatic zones in West Bengal, India where largest Poultry Distribution Scheme of world were implemented. Twenty one variables under three major criteria viz. production and physiological, economical and adoption of technology were considered as vital effect using factor analysis and linear indexing technique. The result showed that 21 day old bird may to be selected for sustainable backyard poultry farming through Rhode Island Red breed at all agroclimatic zone under study. It was shown that a run space of above 20 sq ft per bird with the provision of 50% quantity of commercial poultry ration as per their requirement before laying followed by supplementary feed having 10.01-15.00 g crude protein per day ranked first among 24 treatment groups. The best agroclimatic zone for backyard poultry farming is coastal zone. The best possible combination of input and supportive factor for economic backyard poultry farming at farmers’ doorstep of various agroclimatic zones was also analyzed.
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