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Articles by P. Theerakulpisut
Total Records ( 4 ) for P. Theerakulpisut
  Aroonsri Priprem , S. Radapong , S. Preeprame , P. Chitropas , T. Rittirod , P. Theerakulpisut and P. Chantranothai
  An in vitro permeation of a hydroalcoholic extract of Phyllanthus amarus (PaE) was investigated using excised human epidermis and shed king cobra skin as the barrier membranes. Donor and receptor compartments of diffusion cells were pH–controlled to simulate the permeation environment of the human skin. The PaE was analyzed by using normal–phase densitometric TLC detected at λ 280 nm and toluene:ethyl acetate (17:3) as the mobile phase. There were four major components observed in the saturated solution of the donor at pH 5.5. Over 24 h, only one component, possibly phyllanthin, was found in the receptor solution after permeation across the human epidermis, while two components, possibly phyllanthin and an another unknown permeated, permeated through shed snake skin. When compared to the saturated donor concentration, phyllanthin gave permeation fluxes of 0.04±0.01 and 0.12±0.02 %·cm–2·h–1 through the human and shed snake skins, respectively. It seems that only certain component(s) of the P. amarus extract could permeate through the skins, and by comparison, at a slower rate across the human skin than shed snake skin.
  P. Saleethong , J. Sanitchon , K. Kong-ngern and P. Theerakulpisut
  Environmental stresses including soil salinity are severely affecting rice growth and productivity. The triamine Spermidine (Spd), a member of Polyamines (PAs), a group of phytohormone-like natural amine compounds has been shown to play essential roles in salt stress tolerance in many important crop plants. The effects of exogenously supplied Spd were investigated in two rice cultivars differing in salt tolerance level to determine the roles of Spd on the modulation of physiological parameters related to salt-stress responses. Thirty day-old seedlings of two rice cultivars, Pokkali (salt-tolerant) and KDML 105 (salt-sensitive), grown in nutrient solution were exposed or not to 1 mM Spd for 24 h before submitted to salinized solution containing 150 mM NaCl for 7 days. Salinity stress resulted in significant reduction in plant height, fresh and dry weight of both cultivars but Spd pretreatment significantly increased these growth parameters only in Pokkali. Salt-stress induced considerable disturbance in several physiological processes inhibitory for growth including chlorophyll loss, accumulation of hydrogen peroxide and increase in lipid peroxidation, increased electrolyte leakage and increase in Na+/decrease in K+/Na+. Spd pretreatment led to the reversal of those inhibitory effects in both cultivars. This study showed that exogenous Spd can be applied as short-term pretreatment prior to introduction of salt stress to help elevate salt tolerance of rice and confirmed earlier observations that exogenous Spd offered protective roles on salinity-stressed rice by stabilizing membrane, scavenging free radicals and maintaining K+/Na+ status.
  K. Rattana , P. Theerakulpisut and S. Bunnag
  Abiotic and biotic stresses are the limiting factors of growth and productivity of rice in many agriculture areas of Thailand. Plant Tissue cultures have been used for breeding purpose, especially in selection for stress tolerance. The aim of this study was to investigate and find out enhancement on callus induction and regeneration in rice cultivars; Khao Dawk Mali 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1. The suitable media for callus induction of KDML 105, Supanburi 1, Chai Nat 1 and Pathum Thani 1 were NN medium supplemented with 2, 1.5, 2.5 and 1 mg L-1 2,4-D, respectively. It was found that addition of 300 mg L-1 casein hydrolysate enhanced callus formation in KDML 105. Combination with 300 mg L-1 casein hydrolysate and 1,000 mg L-1 L-proline can influence for callus induction in Supanburi 1. Addition of 500 mg L-1 L-proline can enhance callus induction of Chai Nat 1 and Pathum Thani 1. The MS medium supplemented with 3 mg L-1 BA and 300 mg L-1 casein hydrolysate was suitable for regeneration of KDML 105. The most suitable medium for regeneration in Supanburi 1 was MS medium combination with 3 mg L-1 BA, 0.5 mg L-1 NAA and 500 mg L-1 L-proline. Addition of 5 mg L-1 BA, 0.5 mg L-1 NAA and combination with 300 mg L-1 casein hydrolysate in MS medium were the optimal for regeneration in Chai Nat 1. Combination with 5 mg L-1 BA and 300 mg L-1 casein hydrolysate can influence for regeneration in Pathum Thani 1.
  W. Mahakham and P. Theerakulpisut
  This study is an attempt to contribute and add information on the freshwater algal floral in Thailand. In this study, two members of coleochaetalean green algae, viz., Chaetosphaeridium globosum (Norstedt) Klebahn and Coleochaete nitellarum Jost are reported for the first time for Thailand. Both of them were found as epiphytes on charophyte algae in a mesotrophic reservoir, namely Hui Nam Sab from Amnat Charoen province, Northeast Thailand. Illustrated descriptions of the newly recorded species along with a short note on the species and its distribution are provided. In addition, some ecological aspects of the studied reservoir and coleochaetalean algae are discussed.
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