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Articles by P. Kumar
Total Records ( 12 ) for P. Kumar
  V.K. Gupta , R. Gaur , N. Gautam , P. Kumar , I.J. Yadav and N.S. Darmwal
  The purpose of this study was to characterization of xylanase producing Fusarium solani isolate and optimization of cultural conditions for xylanase enzyme production. Screening of Fusarium solani isolate was based on the diameter of the clear zone formation in oat spelt xylan agar plates, Fusarium solani isolate F7 was selected and optimized for xylanase enzyme production using cheaper substrate like wheat straw, rice straw, rice bran and wood husk. Maximum enzyme activity was observed in wheat straw (78.32 U mL-1). Optimum pH and temperature for xylanase activity were found to be 5.5 and 30° at 3% substrate concentration. In purification step, 75% ammonium sulphate saturation was found to be suitable giving maximum xylanase activity. Purified xylanase yielded single band with a molecular weight of 89 kDa. The use of wheat straw as a major carbon source is particularly valuable because oat spelt xylan is very expensive, The Fusarium solani F7 isolate proved to be a promising microorganism for xylanase production.
  V Subramaniam and P. Kumar

Objective  To assess the effects of chronic tonsillitis with or without adenoiditis and the effects of tonsillectomy with or without adenoidectomy on the voice by means of acoustic analysis.

Design  Prospective case-control study.

Setting  Yenepoya Medical College Hospital, a tertiary referral hospital.

Patients  Patients 5 to 26 years old with chronic tonsillitis with or without adenoiditis.

Interventions  Tonsillectomies were performed under general anesthesia by surgeons using cold steel instruments via a standard capsular dissection technique, and adenoids were removed by curettage.

Main Outcome Measures  Acoustic analysis of 6 parameters (fundamental frequency, jitter, shimmer, harmonics: noise ratio, long-term average spectrum, and nasalance) 4 weeks after surgery compared with 1 day before surgery.

Results  Postoperatively, shimmer altered in males, and hypernasality was eliminated in almost all cases. None of the other associations were significant statistically.

Conclusions  Chronic tonsillitis and tonsillar hypertrophy cause alterations in some acoustic measurements, which make the voice dysharmonic and harsh. Tonsillectomy eliminates nasalance and lowers shimmer. Overall, it does not significantly alter dysphonia owing to disease.

  M. Nagabushanam and P. Kumar
  In this study, micro core engines are designed for computation of predict and update outputs based on lifting scheme 9/7 Discrete Wavelet Transform (DWT) to reduce the time delay. The micro core engines are integrated into pipeline architecture for computation of 1D/2D and 3D-DWT. The 3D-DWT architecture is designed to process a 512x512 image with eight groups of frames sequentially with an improved throughput. The first stage computes 1D-DWT along the rows using four parallel processors. The memory interface with FIFO is implemented to reduce the latency between the first stage and the second stage that computes 2D-DWT along the column. The 3D-DWT is introduced to computes wavelet coefficients in the temporal direction and it is designed to operate at 512x4 clock cycles in sequence with 1D and 2D-DWT computations. The FIFO is implemented at the intermediate stages of the design tosynchronize the data movement in pipeline in all three stages. The memories attached to the input and output of every stage are designed to store the parallel processed data. The proposed architecture is suitable for high frequency and low power applications. Using the 9/7 filter for DWT computation reduces hardware complexity, memory accesses and achieves minimum error during reconstruction of images. The proposed architecture systematically combines hardware optimization techniques to develop a flexible DWT architecture that has high performance and is suitable for portable, high speed, low power applications. The 3D-DWT architecture has been implemented on Virtex-5 FPGA with utilizing of 51% of its slice registers with the frequency of operation is 373 MHz and the designed DWT-IDWT can be used as IP Core.
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
  In the context of the GM food regulations crop improvement via transgenic technology is a new stage of introducing novel food which supercedes over the conventional breeding. It was analyzed that worlds hunger, malnutrition problems, environmental pollution and phytoremediation in agriculture are the challenges for scientist as well as governments those can be combated by application of genetic engineering in crops. Genetically modified microbes/plant/animals or GM microbes/plant/animals results from modification in the genetic make-up of microorganisms, plants and animals using recombinant DNA technology to improve the nutritional requirement, disease resistant traits, increased production and medicinal properties. In many instances, these modification processes represent faster, more efficient mechanisms for achieving changes than traditional breeding. However, a wide variety of modifications are possible through genetic manipulation and the potential for the introduction of toxic compounds, unexpected secondary effects and changes in nutritional and toxicological characteristics may give rise to safety concerns about GM crops. Thus, generation of GM food explores new vistas for future food requirement but the assessment of policy regarding environmental risks is also to be concerned.
  V. Rai , U. Yadav , P. Kumar and S. Gupta
  The present study was aimed to analyze methionine synthase reductase (MTRR) A66G polymorphism in Uttar Pradesh (UP) population and PCR-RFLP method was used for the mutation analysis. Total 104 samples were analyzed and AA genotype was found in 10 individuals, AG genotype in 67 individuals and GG in 27 individuals. The frequencies of AA, AG and GG genotypes in the present study were also assessed with the expected distribution (i.e. Hardy-Weinberg equilibrium) by using χ2 test. The genotype frequencies of AA, AG and GG were 0.096, 0.644 and 0.259, respectively. Allelic frequencies of A and G were 0.418 and 0.581. It was reflected from the results of the present study that the percentage of heterozygous genotype (AG) is highest in the target population.
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
  P. Coronel , J. Simunovic , K. P. Sandeep and P. Kumar
  Dielectric properties of pumpable food materials having a potential to be processed using a continuous flow microwave heating system were measured at 915 MHz and in the temperature range of 10-90°C. The products considered in this study were milk and dairy products (ε`: 70.0 to 50.8 and ε``: 14.7 to 41.3), ready to eat puddings (ε`: 69.4 to 52.1 and ε``: 17.2 to 23.8), soy beverages (ε`: 75.4 to 60.8 and ε``: 9.0 to 19.8), and avocado products (ε`: 51.6 to 39.0 and ε``: 17.7 to 67.5). The results showed that the dielectric constant decreased with an increase in temperature and the dielectric loss factor increased with an increase in temperature. Polynomial correlations for the dependence of dielectric properties on temperature were developed. The dielectric properties measured in this study are important parameters for designing a continuous flow microwave heating system for processing pumpable food materials.
  P. Kumar , A. Semalty , S.R. Mir , M. Ali and S. Amin
  The plant Pongamia pinnata (Linn.) Pierre of family Leguminosae sub-family Papilioanaceae was evaluated for its hypoglycemic and hypolipidemic activity in streptozotocin induced diabetic rats. A new difuranoflavonone Compound PP (named Pongamiaflavonol), isolated from methanlolic extract of P. pinnata pods by column chromatography, was also studied for the activity. It was observed that after 14 days of treatment blood glucose level was reduced by 66.34, 54.82, 63.62 and 67.48 % with Std. Glibenclamiade 3 mg kg-1), P. pinnata pods (300 mg kg-1), P. pinnata flowers (300 mg kg-1) and PP (100 mg kg-1), respectively. The lipid profile was also studied and was found to be normalized significantly by both the flowers and pods extracts of P. pinnata and compound PP.
  A. Semalty , M. Semalty , P. Kumar , S.R. Mir , M. Ali and S. Amin
  Pongamia pinnata (family Papilionaceae) has been used for bronchitis, whooping cough, rheumatic joints and quench dipsia in diabetes. This study deals with the isolation of a new hypoglycemic phytoconstituent from P. pinnata pods. The hypoglycemic activity of the isolated phytoconstituent was evaluated in comparison of the methanolic extract of the pods.. Methanolic extract of P. pinnata pods was fractionated by column chromatography and the isolated compounds were identified by spectral analysis. A new compound named Pongamiaflavonylflavonol was isolated from chloroform: methanol (97:3) eluant. This new isolated compound was studied for hypoglycemic activity in streptozotocin induced diabetic rats. Methanolic extract of P. pinnata pods and pongamiaflavonylflavonol showed significant hypoglycemic activity in streptozotocin-induced hyperglycemic rats after oral administration. At the end of 6 h the new compound showed 12.15% reduction in blood glucose level in comparison of extract (11.36%) against the standard (16.93%). It can be concluded that the novel Pongamiaflavonylflavonol isolated from P. pinnata pods may be useful as oral hypoglycemic therapeutic agent. This may serve as a lead compound for development of more potent drugs for clinical use in diabetes.
  P. Kumar , N. P. Shah , A. Singhal , D. S. Chauhan , V. M. Katoch , S. Mittal , S. Kumar , M. K. Singh , S. Datta Gupta and H. K. Prasad
  Endometrial biopsy samples derived from 393 patients with assorted gynecological complaints were investigated for mycobacterial infection. By employment of four different techniques, mycobacterial pathogens were detected irrespective of the nature/type of clinical complaint. Mycobacterium tuberculosis was the predominant pathogen detected among the samples investigated.
  S. Ari , P. Kumar and G. Saha
  The first step towards detection of valvular heart diseases from heart sound signal (phonocardiogram) is segmentation. A segmentation algorithm provides the location of the first and second heart sounds which in turn helps to locate and analyse the murmur. Established phonocardiogram based segmentation methods use an electrocardiographic (ECG) signal as a continuous auxiliary input in a complex instrumentation setup. This paper proposes an automatic segmentation method that does not require any such auxiliary signal. Compared to other approaches without auxiliary signal, this work extensively utilizes biomedical domain features for reduction of time and computational complexities and is more accurate. The performance of the algorithm is evaluated for nine commonly occurring pathological cases and normal heart sound for various sampling frequencies, recording environments and age group of subjects. The proposed algorithm yields an overall accuracy of 97.47% and is compared with two competing techniques. In addition, the robustness of the algorithm is shown against additive white Gaussian noise contamination at various SNR levels.
  A. Sharma , P. Kumar , M.M. Singh and N. Vasishta
  A brief retrospective analysis of thirty small ruminants reared in north western Himalayas, suffering from dystocia were evaluated over a period of two years in Teaching Veterinary Clinical Complex, Palampur, India. The clinical cases of dystocia were categorized into fetal (n = 11) and maternal causes (n = 19). Fetal causes included faulty maldisposition (n = 5), fetal monster (n = 2) and oversized fetus (n = 4). Similarly, maternal causes includes incomplete cervical dilatation (n = 8), uterine torsion (n = 2), narrow pelvis (n = 7) and secondary uterine inertia (n = 2). Thirteen animals were relieved from dystocia by gentle traction and rest seventeen animals were subjected to caesarean section by lower left flank laparohysterotomy. All does except three, had an uneventful recovery. Only 21.62% (n = 8) live kid/lamb were delivered by traction or caesarean section procedures adopted. In our study major causes of dystocia due to fetal and maternal origin were faulty maldisposition (45.45%) and incomplete cervical dilatation (42.10%). The 60% (18/30) of dystocia were present in yearling or primiparous females.
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