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Articles by Otilia BOBIS
Total Records ( 10 ) for Otilia BOBIS
  Liviu Al. MARGHITAS , Olimpia MORAR , Otilia BOBIS , Victorita BONTA , Daniel DEZMIREAN and Melinda TOFALVI
  The article presents the researches carried out with fresh royal jelly in different larvae stage. At the beginning, the initial material was analyzed on the content of nutritious substances. This type of work is extremely valuable for practical application in food science, therapy and nutritive supplements. In scientific word royal jelly has been accepted and used as nutritive substance complex, benefic for human’s health. The primary objective of this study was to evaluate the physicochemical parameters of different samples of royal jelly in different larvae stage. The principal constituents of royal jelly are water, protein, sugars, lipids and mineral salts. Although they occur with notable variations, the composition of royal jelly remains relatively constant when comparing different colonies, bee races and time of harvesting.
  Otilia BOBIS , Liviu Al. MARGHITAS , Daniel DEZMIREAN , Olimpia MORAR , Victorita BONTA and Flore CHIRILA
  The aim of this study was to determine the composition and microbiological quality of commercially honeybee-collected pollen, bee bread and royal jelly and to define the following physicochemical and microbiological characteristics: water content/dry weight, protein content, fat content, sugar spectrum and some microbiological parameters. The main sugars identified by high performance liquid chromatography were fructose, glucose, and sucrose, with small amounts of disaccharides. Among the microbiological parameters, a relatively high number of moulds and total aerobic counts were found in fresh pollen, but under the maximum admitted level.
  Liviu Al. MARGHITAS , Victorita BONTA , Daniel DEZMIREAN and Otilia BOBIS
  The aim of the present work was the development, validation and implementation in routine analysis of a technique that guarantees low detection limit for chloramphenicol in honey using liquid chromatography (LC)-mass spectrometry (MS) with photodiode array (PDA) detection. Sample preparation was carried out by liquid-liquid extraction of a honey solution in phosphoric buffer (PBS, pH=7.8). The determinations were performed using a Zorbax Eclipse XDB-C18 column and gradient mobile phase, using an electrospray ionization source (ESI) in negative ion mode. Recoveries were calculated at three concentration levels and higher values than 85% were obtained, with relative standard deviations less than 7.7%. The applicability of the present method was tested on 12 honey samples purchased from different beekeepers from Transylvania.
  Daniel DEZMIREAN , Liviu Al. MARGHITAS , Otilia BOBIS , Laura STAN , Victoriţa BONTA , Ovidiu MAGHEAR , Georgeta DINITA and Olimpia MORAR
  Romanian Calluna vulgaris honey that came from Cluj and Salaj County, the most important Calluna areas in Romania was evaluated for authenticity by pollen-analysis, several physical-chemical analyses, including sugar profile and mineral content. Physical-chemical and mineral content analyses show clearly that the samples taken for analysis were pure Calluna vulgaris honeys. This type of honey was found to have the highest potassium content from all the honeys in Romania that were analyzed in our laboratory. Statistical data were processed in order to develop a biochemical profile of this type of honey.
  Rodica MARGAOAN , Liviu Al. MARGHITAS , Daniel DEZMIREAN , Cristina M. MIHAI and Otilia BOBIS
  Pollen represents the male reproductive cells collected by honeybees from flower stamens. The nutritional and medicinal value of bee pollen is known for centuries. With orange to red pigments, it provides nutrition through its considerable amounts of proteins, sterols, vitamins, fatty acids, phenol compounds and other physiologically active compounds. As a healthy bee product, pollen is considered to be the first-rate reservoir of antioxidants. Worldwide interest in bee pollen has recently developed as more information on their chemical composition and physiological effects has become highlighted. Bee collected pollen is regarded as valuable special food, having different health enhancing effects, also used in apitherapy. Its value is given by the content in amino acids, lipids, carbohydrates, mineral salts, vitamins, polyphenols, carotenoids, which depends on the botanical source available to bees.
  Otilia BOBIS , Liviu Al. MARGHITAS , Daniel S. DEZMIREAN , Flore CHIRILA and Robin F.A. MORITZ
  Different types of Romanian honeys were evaluated for their antioxidant and antibacterial activity. Total polyphenols, total flavonoids and two colorimetric methods for radical scavenging activity and the total antioxidant power showed different amounts of biologically active compounds in tested honey samples. Antibacterial activity was tested against Escherichia coli and Paenibacillus larvae, the first a model organism for bacteria and other a pathogenic bacterium causing American foul brood disease in Apis melliefra. Heather and honeydew honey had the highest content of total polyphenols (127.35 and 123.28 mgGAE/100 g and), while sunflower honey together with honeydew honey show the highest content of total flavonoids (64.25 and 65.98 mgQE/100 g honey). The most effective in antibacterial activity for P.larvae was found in sunflower honey whereas multifloral honey showed highest activity against E.coli.
  Lavinia I. BARNUŢIU , Liviu Al. MARGHITAS , Daniel S. DEZMIREAN , Otilia BOBIS , Cristina M. MIHAI and CrenguNa PAVEL
  This paper contents a summary about antimicrobial compounds from royal jelly. Royal Jelly is a nutritive secretion produced by the hypopharyngeal and mandibular glands of worker bees; it is the main nutritional font of the queen honeybee and contains vitamins, carbohydrates, minerals and proteins. It possesses a number of pharmacological activities and is widely used in commercial medical products, health foods, and cosmetics in many countries. Peptides isolated from natural fonts are the object of several studies aimed at finding new molecules possessing antibacterial activity.
  Daniel S. DEZMIREAN , Liviu Al. MARGHITAS , Otilia BOBIS , Helge SCHLUNS , Cristian AURORI , Cristian COROIAN , Ellen SCHLUNS , Emilia FURDUI , Bogdan GHERMAN , Lavinia BARNUTIU , Cristina MIHAI , Ovidiu MAGHEAR , Laura STAN and Robin MORITZ
  The main objective of the project RoBeeTech is to create a high competence level scientific center of bee biotechnology in Romania. The research directions of RoBeeTech are similar to actual research interests of international teams in the field of bee pathology, bee products quality or the genetic variability analysis of bees through modern biotechnologies. The three main research directions involve the two research labs from USAMV Cluj-Napoca -APHIS (Laboratory for Quality Control of Bee Products) and DIA (Laboratory for Bee Disease Diagnosis). The objectives of RoBeeTech are focused on identification of genetic variability of bee colonies from Romania, the study of bee-parasite-pesticide interaction and new control and treatment techniques based on biologically active effect of bee products and secondary metabolites from plants. Until now bee samples from over 80% of the country were collected, two progress reports and two repayment documents were already delivered and 4 articles were published at international and national conferences.
  Adela MOISE , Liviu Al. MARGHITAS , Daniel S. DEZMIREAN , Otilia BOBIS and Ovidiu MAGHEAR
  A theoretical study about the heather honey was done in order to establish its main characteristics. This research represents a part of a large study regarding the plant Calluna vulgaris and the honey obtained by bees harvesting its nectar. Many bibliographical papers were studied in order to apply in laboratory the appropriate methods for quality control and markers determination of heather honey. The study focused especially on the papers regarding the physical-chemical parameters, the melissopalynological exam, the mineral content and the chemical markers of heather honey.
  Crenguta I. I. PAVEL , Liviu Al. MARGHITAS , Daniel S. DEZMIREAN , Otilia BOBIS , Lavinia BARNUTIU and Agripina SAPCALIU
  Royal jelly is a secretion product of the cephalic glands of nurse bees that is very sensible to the storage conditions. The aim of this study is to review the changes that occur in royal jelly composition during storage and possible freshness parameters that have been studied. Evaluation of coloration, viscosity, water-soluble protein fraction, enzyme glucose oxidase, furosine, 10-HDA was proposed to assess the RJ freshness. Actually, royal jelly is one of the most studied bee products, but further studies are necessary in order to establish an international standard of the product and its freshness parameters.
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