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Articles by O.A. Abdalla
Total Records ( 5 ) for O.A. Abdalla
  S.M.T. Mohammed , M. Humidan , M. Boras and O.A. Abdalla
  The aim of this study was to determine the effect of growing tomato on different root stocks on its growth and yield. In this study, tube grafting method was adopted since it has been widely used with high percentage of success. Tomato (Lycopersicon esculentum Mill.) cultivar Cecilia F1 was grafted using tube grafting method on three rootstocks, Beaufort, He-man and local Syrian tomato which were grown under glasshouse and fertilized according to the routine fertilization program with macro and micronutrients in ASTRA farms, Tabuk, north-west of Saudi Arabia. Plants produced from grafting Cecilia F1 scion on Beaufort rootstock were the tallest (37.56 cm) and had the greatest number of leaves (7.22) and stem diameter (4.92 cm). Six weeks after of grafting seedling leaves contents of Ca, Na, Mg, Fe and K increased and while the roots contents were not affected. Chlorophyll a and carotenoids significantly increased. The productivity of grafted tomato planted in glasshouse increased significantly and had reached up to 21%. Grafting also increased Total Dissolved Solids (TDS) and decreased the amount of lycopene in all grafts but β-carotene increased in Cecilia on Beaufort (5.46 mg kg-1) and decreased in both Cecilia on He-man and Cecilia on local Syrian tomato fruits. It is concluded from this study that grafting significantly affected tomato growth and yield depending on the different rootstocks utilized.
  M.A. AL-Saleh , I.M. Al-Shahwan , O.A. Abdalla and M.A. Amer
  The goal behind this study was the identification, biological and molecular characterization of a virus infecting garden rocket (Eruca sativa Mill) and producing severe symptoms on it in Riyadh region, Saudi Arabia. Mosaic, stunting and deformation were observed on field grown garden rocket plants. Mechanical inoculation of twelve plant species with sap prepared from infected garden rocket plants resulted in infection of four of them. These were E. sativa Mill, Raphanus sativus L., Brassica rapa L. and Chenopodium ammaranticolor Cost and Reyn L. Symptoms on the first three plant species were systemic mosaic, stunting and deformation, whereas symptoms on C. ammaranticolor were local lesions. Brevicoryne brassicae L., transmitted the virus to E. sativa in a non-persistent manner. Electronmicroscopic examination of carbon-coated grids prepared by the leaf dip method revealed flexuous virus particles typical of potyviruses. Enzyme linked immunosorbent assay indicated that samples collected from symptomatic E. sativa plants in both Riyadh and Qassim regions were infected with turnip mosaic potyvirus (TuMV). Positive results were also obtained using a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method to detect and identify TuMV from nucleic acid extracts of the symptomatic garden rocket plants collected from that area, using a specific oligonucleotide primer for detection of TuMV-CP. Nucleic Acid Spot Hybridization Assay (NASH) using DIG labeled cDNA probe showed high levels of hybridization signal, whereas no hybridization was observed with uninfected tissues. The nucleotide sequence of the CP gene of the Saudi Arabian isolate of TuMV detected in garden rocket and tentatively denoted (TuMV-SA-Ro) was determined to be composed of 862 nucleotides in length.
  M.A. AL-Saleh , I.M. Al-Shahwan , O.A. Abdalla and M.A. Amer
  S.M.T. Mohammed , M. Humidan , M. Boras and O.A. Abdalla
  The objective of this study was to determine the impact of interaction between rootstocks and scions of watermelon and tomato on the chemical contents of their leaves and roots. The rootstock of local Syrian tomato produced the highest amount of total lipids, total fatty acids percentage and total unsaponated percentage of total lipids in root (0.69, 92.39, 3.34%) and leaf (0.73, 91.54, 4.02%) compared to Beaufort rootstock and He-man rootstock when grafted with Cecilia scion. With regard to watermelon grafts Samara on C. pepo excelled the two other watermelon grafts namely, Samara on Tetsukauto and Samara on Lagenaria siceria in its overall root contents of total fatty acids percentage of total lipids and total unsaponated percentage of total lipids. Samara on C. pepo excelled the other two watermelon grafts with regards to its significant leaves (0.1326, 18.73%) and root contents (0.1214, 15.33%) of phospholipids percentage and total unsaponoted phospholipids, respectively. Grafting increased indole-3-acetic acid (IAA) in seedlings of both tomato and watermelon. Cecilia on Beaufort (32.43 nm g-1) and Cecilia on Syria (36.71 nm g-1) had significantly greater contents of Indole-3-acetic acid in their roots whereas, only Samara on C. pepo watermelon grafts (98.27 nm g-1) had significant greater contents of IAA in its roots. Clear effects of grafting watermelon and tomato on their lipids and IAA contents were concluded in this study.
  M.A. AL-Saleh , I.M. AL-Shahwan , M.A. Amer and O.A. Abdalla
  This study aimed at identifying the causal agent inducing virus-like symptoms on radish and lettuce plants in Riyadh Region, Saudi Arabia. Mottling, chlorosis and mosaic symptoms were observed on lettuce (Lactuca sativa Linn.) and radish (Raphanus sativus Linn.) plants in two areas in Riyadh region. Mosaic symptoms were observed on L. sativa, R. sativus, Eruca sativa Mill and Brassica rapa Linn., whereas local lesions were observed on Chenopodium ammaranticolor Coste and Reyn., which were mechanically inoculated with sap from infected radish and lettuce plants. Electron microscopy revealed filamentous flexuous particles typical of potyviruses. The aphid Brevicoryne brassicae transmitted the virus to lettuce and radish in a non-persistent manner. Turnip mosaic virus (TuMV) was the only detected virus by double antibody sandwich-enzyme linked immunosorbent assay among five viruses suspected of such disease symptoms. Amplification of total RNA extracted from infected lettuce and radish plants yielded 1-1.2 kbp using three degenerate primers which were designed for potyvirus group detection and 985 bp complementary DNA (cDNA) fragments using a specific oligonucleotide primer for TuMV detection. Nucleic acid sequence analysis of TuMV-L-SA revealed a range of 85.3 to 90.9% sequence identity with other TuMV isolates obtained from the GenBank. However, the TuMV-Ra-SA revealed a range of 87.7 to 94.1% sequence identity. The sequence similarity between TuMV-L-SA and TuMV-Ra-SA investigated in the current study was 89%. To our knowledge, this is the first report of TuMV on lettuce and radish in Saudi Arabia.
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