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Articles by Nisar Ahmad
Total Records ( 3 ) for Nisar Ahmad
  Nisar Ahmad , Iqbal Munir , Imtiaz A. Khan , Waqar Ali , Wisal Muhammad , Rakhshanda Habib , Raham Sher Khan and Zahoor A. Swati
  The most challenging hurdle facing Pakistan is the production of Brassica germplasm with a wider genetic base and using them properly in rapeseed genetic improvement. Genetic diversity was evaluated in 20 rapeseed lines (10 entries each of B. napus and B. campestris) using RAPD as molecular markers. Four Randomly Amplified Polymorphic DNA primers were used to estimate the genetic distances among the genotypes in all the possible combinations. The genetic diversity study revealed different levels of polymorphism for RAPD primers GLA07, GLB07, GLD18 and GLE07 that resulted in amplification of 3.2, 1.5, 3.0 and 3.5 scorable bands (loci) per genotype of Brassica napus and 2.5, 1.3, 2.6 and 3.7 scorable bands (loci) per genotype of B. campestris. Among Brassica napus genotypes, maximum genetic distance (79%) was observed between Torch+Maluka, Torch+Baro and Torch+Global, while, maximum genetic distance (91%) was observed between T-16 and P1-367601 genotypes of Brassica campestris. Individual genetic distance observed among the B. napus and B. campestris lines ranged from 21.50 to 59.41% and 53.75 to 60.09%, respectively. The dissimilarity coefficient matrix of these lines based on the data of four RAPD markers using UPGMA (Unweighted Pair Group of Arithmetic Means) method was also used to construct a dendrogram. The dendrogram analysis indicated that lines Torch and 366822 of B. napus, while 2163 and P1-392029 of B. campestris were genetically apart from other lines. These results provide valuable information for fingerprinting that can be used in a synergistic way to create wider genetic base and augment the breeding program of Brassica in Pakistan.
  Muhammad Yaseen , Nisar Ahmad , Abdul Razzaq and Nasir Mahmood
  A laboratory experiment was conducted to study the effect of incubation period on the phosphorus adsorption by the Pindorian, Satghrah, Shandara, Wazirabad, Balkassar, Hafizabad and Missa soil series of Pakistan. Five levels of P (50, 100, 200, 400 and 800 μg P/g of soil) were added to these samples and incubated for 1, 7 and 15 days. Results showed that the amount of adsorbed P increased by increasing the incubation period from 1 to 15 days. The highest adsorption was found after 15 days incubation period and lowest P was adsorbed after incubation period of one day in all the soils and each level of P application.
  Zahida Tasawar , Zeshan Siraj , Nisar Ahmad and Mushtaq H. Lashari
  The present study has been carried out to determine the effects of Nigella saitva on the lipid profile in cardiac patients visited at Ch. Pervaiz Elahi Institute of Cardiology, Multan. The age of the subjects was 26-69 years. The eighty subjects were divided into two groups (interventional and non-interventional) through random stratification (n = 40/group) by weight. The interventional group given Nigella sativa and statin, non-interventional group given statin daily. Both groups were advised to take the recomended doses regularly for a period of six months and the patients were on usual care. Fasting blood samples were taken before and after two and six month’s treatment. In interventional group, cholesterol decreased by (-14.58%), LDL (-23.00%), VLDL (-15.16%) and triglycerides (-15.16%) significantly (p<0.05) after the treatment, whereas there was significant increase (p<0.05) in HDL cholesterol (3.18%) after six months. In non-interventional group the cholesterol decreased by (+1.17%), LDL (-4.13%), VLDL (-3.10%) and triglycerides (-2.12%) non significantly (p>0.05) after the treatment, whereas there was significant increase (p<0.05) in HDL (+5.87) after six months. In conclusion, the Nigella sativa is effective to change the lipid profile significantly in a way which is beneficial to heart.
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