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by
Ngadikun |
Total Records (
2 ) for
Ngadikun |
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Prasetyastuti
,
Adhi Tanjung Laksono
,
Ricky Taufiqurrohman
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Tantra Dewi Rahardyanti
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Gandhi Anandika Febryanto
,
Yan Mahmud Fau
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Sri Herwiyanti
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Ngadikun
and
Sunarti
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Background and Objective: Carbon tetrachloride (CCl4), a carcinogenic compound commonly used in household cleaning agents and spot removers can cause free radical-mediated liver damage. Recent research shows polyphenols in pomegranate (Punica granatum L.), rich in exogenous antioxidants, may protect the liver from hepatotoxic damage. This study aimed to investigate the effect of pomegranate juice on CCl4-induced liver damage in a rat model. Materials and Methods: A total of 25 rats, aged 8 weeks and weighing an average of 200 g were used. They were divided into 5 groups as follows: (1) Normal, (2) Hepatotoxic rats and (3), Hepatotoxic rats with pomegranate juice 0.4 mL/200 g body weight (BW) (4) Hepatotoxic rats with pomegranate juice 0.8 mL/200 g BW and (5) Hepatotoxic rats with pomegranate juice 1.2 mL/200 g BW, respectively. Hepatotoxicity was done by intraperitoneal injection of 0.5 mL–1 CCl4 kg–1 BW for 5 days. Before and after 2 weeks administration of treatment, blood samples were collected for assessment of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (γ-GT) and malondialdehyde (MDA). At the end of the study, the animals were euthanized and the livers were used for histopathological assessment. Data were analyzed by paired t-test and one-way ANOVA followed by the Games-Howell test. Results: Supplementation with three different doses of pomegranate juices in CCl4-induced rats significantly reduced the serum levels of ALT, AST, ALP, γ-GT and MDA (p<0.05) and pomegranate juice as much as 0.8 and 1.2 mL/200 g BW reduced liver damage in CCl4-induced rats. Conclusion: According to findings of this study supplementation with pomegranate juice can improve liver damage induced by CCl4 in rats. |
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Prasetyastuti
,
Mochammad Willy Pratama Anthony
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Nuha Aulia Rahman
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Ngadikun
and
Sunarti
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Diabetes mellitus is a metabolic disorder that is characterized by hyperglycemia and associated with increased oxidative stress. The polyphenol property in pomegranate juice was reported to have significant antioxidant effect. The present study aims to investigate the hypoglycemic and antioxidative effect of pomegranate juice in streptozotocin-induced diabetic rats. Twenty healthy male rattus norvegicus with age of eight weeks and weighing at average of 200 g were randomly assigned into four groups. Diabetic models were induced by using 60 mg/kg body weight (BW) streptozotocin (STZ) intraperitoneally. The rats were allocated into four groups, group treated with 0.1 mg/kg BW glibenclamide, groups supplemented with 1, 2 and 4 mL/200 g BW pomegranate juice, respectively. Evaluation of hypoglycemic and antioxidative effect were performed by measuring blood glucose level, lipid peroxidation, including malondialdehyde (MDA) and advanced glycation end-product (AGE) before and after treatment. Lipid profile was also measured before and after treatment. Treatment with glibenclamide and supplementation with three different doses of pomegranate juice in diabetic rats were significantly reduced blood glucose level. Supplementation with 2 mL/200 g BW pomegranate juice was significantly reduced MDA concentration, whereas the AGE concentration was not significantly decreased by pomegranate juice supplementation. Lipid profile related to total cholesterol and triglyceride levels were not significantly reduced. Furthermore, high density lipoprotein was not increased significantly after each treatment. Pomegranate juice has hypoglycemic and antioxidative effects in streptozotocin-induced diabetic rats. |
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