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Articles by Neha Behar
Total Records ( 2 ) for Neha Behar
  Neha Behar , K.L. Tiwari and S.K. Jadhav
  Curcuma caesia Roxb. belonging to family Zingiberaceae is an important traditional medicinal plant. The rhizome has been widely used as folklore medicine. Chhattisgarh state medicinal plant board has categorized it as an endangered plant. Turmeric/ginger is vegetatively propagated exclusively through underground rhizomes and multiplication rate is very low. Furthermore, susceptibility to diseases, specifically soft rot caused by Pythium species causes heavy losses. In vitro micropropagation technique can be useful for conservation and commercial exploitation of valuable secondary metabolites in medicinal plants. In vitro plant regeneration system depends upon many factors but the type of explants has been identified as one of the major factor. Thus, the present study was conducted using various parts of the plant viz. leaf, root, rhizome sections, mature bud of rhizome and sprouted bud of rhizome in different hormone concentration and combinations in MS medium. Only mature bud and sprouted bud from the rhizome responded while others did not show any sign of morphogenesis. Sprouted and mature bud showed best response in 4 mg L-1 BAP+100 mg L-1 ADS with mean 3.8±0.32 number of shoots and mean 3.28±0.42 cm length, similarly, 1±0.39 mean shoots and mean 1.17±0.48 cm length in mature bud. In vitro produced plants were easily established in soil with almost 100% survival and were morphologically similar to their parent plants. Thus, sprouted buds of the rhizome can be exploited for further micropropagation and conservation studies.
  Neha Behar , K.L. Tiwari and S.K. Jadhav
  The traditional system of medicine involves the use of different plant extracts or the bioactive constituents for treating various ailments. It is important to establish and compare the phytochemical profiles of lesser known medicinal plant (C. caesia) with the well-known species of the genus (C. longa) for phytochemical similarities or dissimilarity for further drug formulations in this unexplored plant. Thus, this study was conducted which involved soxhlet extraction from leaves and rhizome of both the plant using methanol and chloroform solvents, phytochemical testing for identification of secondary metabolites and thin layer chromatography profiling for presence of curcuminoids. In Curcuma longa, the methanolic extract of rhizome yielded 39.8% while in Curcuma caesia, yield was 6.4%. In Curcuma caesia alkaloids and tannins were found in addition to other metabolites while they were absent in Curcuma longa. In TLC profiling, the rhizome of both the Curcuma species showed presence of curcuminoids while in leaves only methanolic extract in C. longa showed distinctable bands for curcuminoids and in C. caesia, leaves showed only presence of curcumin while dis-methoxycurcumin and bis-demethoxycurcumin was found absent. Presence of medicinally important bioactive compounds in Curcuma caesia depicts that it has great potential for becoming a future drug.
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