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Articles by Nazila Pourkhalili
Total Records ( 3 ) for Nazila Pourkhalili
  Mojdeh Mohammadi , Shokoufeh Atashpour , Nazila Pourkhalili , Amir Nili-Ahmadabadi , Maryam Baeeri , Azadeh Mohammadirad , Shokoufeh Hassani , Shekoufeh Nikfar and Mohammad Abdollahi
  Increased oxidative stress plays a role in the pathogenesis of cellular death and β-cell failure. The aim of this study was to evaluate the action of different types of phosphodiesterase (PDE) inhibitors including milrinone (PDE-3), rolipram (PDE-4) and sildenafil (PDE-5) on viability, production of Reactive Oxygen Species (ROS) and secretion of insulin from isolated rat pancreatic islets. Pancreatic islets were carefully isolated and incubated in RPMI 1640 for 24 h. After overnight incubation, islets were picked up and divided into ten in each groups. Then, milrinone, rolipram and sildenafil at doses of 0.1, 1, 10 and 100 FM were added to islet groups and incubated for further 24 h. Then static insulin secretion at 2.8 and 16.7 mM concentrations of glucose, was tested. Then the viability of cells, level of ROS and insulin were examined. The results of static experiments showed that secretion of insulin increased significantly in response to glucose at both basic (2.8 mM) and stimulation (16.7 mM) levels by the lower doses of tested PDE inhibitors. The level of ROS at the lower doses of milrinone decreased. The viability of islets at the lower doses of all of PDE inhibitors were increased; however, viability at the higher doses of sildenafil and rolipram reduced significantly. Milrinone was the most effective PDE inhibitors on the function of isolated pancreatic islets. PDE inhibitors show the most significant anti-oxidative effects at lower doses. Concerning improvement of isolated islets function, PDE-3 inhibitor is the best among tested compounds. PDE inhibitors may help management of diabetes and facilitate conditions of islet transplantation.
  Mona Navaei-Nigjeh , Mahban Rahimifard , Nazila Pourkhalili , Amir Nili-Ahmadabadi , Mohsen Pakzad , Maryam Baeeri and Mohammad Abdollahi
  Oxidative stress is involved in complications of diabetes. This study investigated the hypothesis that the cerium oxide nanoparticle/sodium selenite combination can synergistically improve oxidative stress indexes in vital organs (kidney, heart, brain and lung) of diabetic rats. Diabetes was induced in overnight-fasted male Wistar rats via a single dose of streptozotocin (STZ, 60 mg kg-1). The effective doses of cerium oxide nanoparticle (60 mg/kg/day) and sodium selenite (5 μmol/kg/day) alone or in combination were administered for 14 days to diabetic rats. Rats with blood glucose of more than (300 mg dL-1) were selected and divided into six groups including vehicle control, STZ control, cerium oxide nanoparticles, sodium selenite, combination of cerium oxide nanoparticles with sodium selenite and metal form of cerium oxide. At the end of 2 weeks, organ tissues including brain, heart, lung and kidney of animals were removed and then oxidative stress markers including cellular Lipid Peroxidation (LPO), Total Antioxidant Power (TAP), Total Thiol Molecules (TTM) and Reactive Oxygen Molecules (ROM) were evaluated. Combination of cerium oxide nanoparticles and sodium selenite significantly reduced ROM and LPO levels in all the organs. The results of TTM showed an increase in all tissues expect the lung. TAP increased in combination group in all studied tissues expect the lung. The beneficial effect of cerium oxide nanoparticles/sodium selenite in diabetic rats is mediated through control of oxidative stress mechanisms. These effects were more noticeable in kidney, brain and heart.
  Mahban Rahimifard , Mona Navaii-Nigjeh , Amir Nilli-Ahmadabadi , Nazila Pourkhalili , Maryam Baeeri , Azadeh Mohammadirad and Mohammad Abdollahi
  Glycyrrhizic Acid (GA) a major component of licorice, has been reported to have potent antioxidant effects and used widely throughout the world. In the present study, the effects of GA on the function, viability and level of Reactive Oxygen Species (ROS) in isolated rat pancreatic islets were evaluated. After Laparotomy, pancreas was removed and islets were isolated and incubated in RPMI 1640 for 24 h and then islets were separated. GA at logarithmic doses (1, 10, 100 and 1000 μM) were added to islets and incubated for 24 h and then static insulin secretion was tested. Also, viability of cells and their ROS level were determined using Mitochondrial Toxicity Test (MTT) and fluorometric assay. Then islets were stained by dithizone and observed under microscope. The results of MTT test indicated that rang of 1-100 μM of GA is safe. In the dose of 1000 μM, GA increased ROS and reduced viability of islets. GA at 1 μM significantly increased secretion of insulin via isolated islets in the presence of stimulation level of glucose (16.7 mM). Results of dithizone staining showed a reduction in live cells at high dose of GA. The LC50 study was done to determine the toxicity of GA on rat pancreatic islets and a 24 h LC50 of 15 mM was found. GA showed remarkable anti oxidative effects at low doses and improved islet’s viability and insulin secretion in stimulation level of glucose. Interestingly, high dose of GA induced oxidative stress and reduced function of islets. The results of the present study indicate that GA is a good candidate to be examined in islet transplantation procedures to maintain islets viable and functional.
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