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Articles by Naheed Mojgani
Total Records ( 2 ) for Naheed Mojgani
  Naheed Mojgani and Cyrus Amirnia
  L. casei RN 78 isolated from a dairy sample was shown to produce a proteinaceous substance inhibitory towards a number of gram positive bacteria including an important food-borne pathogen L. monocytogenes. The inhibitory substance produced by this strain appeared not related to organic acids and hydrogen peroxide and was found resistant to catalase, lipase and lysozyme. The proteinaceous antagonistic nature of the agent appeared to be related to a bacteriocin and was termed Lactocin RN 78. The stability of the crude Lactocin RN 78 in the presence of different physical and chemical factors was evaluated which indicated its stability at wide temperature and pH range. The spectrum of inhibition of this bacteriocin was further enhanced to the growth of E. coli, Ps. aeruginosa, and S. typhi in the presence of 1% chelating agents like SDS and EDTA. Lactocin RN 78 appeared stable in the presence of high concentration of NaCl and resisted 40% of this salt during 48 h of incubation at room temperature. The kinetics of Lactocin RN 78 production by L. acidophilus RN 78 as a function of cellular condition was evaluated, which indicated a direct relationship between the growth rate (cfu mL-1) and the amount of bacteriocin produced. The synthesis and inhibitory activity started in the early logarithmic phase and continued to the end of exponential phase. The growth became static after entering the stationary phase within 10 h of incubation at 35°C and the antibacterial titer remained constant thereafter. The influence of variable physical and chemical agents on the growth metabolism and Lactocin RN 78 synthesis together was investigated. The optimal temperature and pH favoring maximum growth and production in the producer strain was 35°C and 6.0, respectively. The producer strain was able to grow and produce Lactocin RN 78 in the presence of 3% NaCl and 0.3% bile salts. The growth medium most effective was MRS broth medium supplemented with a carbon source.
  Naheed Mojgani and Mehdi Perveen Ashtiani
  A broad-spectrum bacteriocin produced by L. casei RN 86 was a stable and relatively small molecule (5.5 KDa) being bactericidal againsta range of mastitis-causing bacterial pathogens. The antimicrobial activity of the peptide against the sensitive cells was 10 fold increased (AU mL-1) after partial purification by 20% ammonium sulphate precipitation. The partially purified peptide indicated to be bactericidal against S. aureus and S. agalactiae cells and killed approximately 106 to 109 of the actively growing sensitive cells within an hour of incubation. It caused rapid cessation of the growth of the sensitive strains within few minutes of contact, while almost 99.9% of the cells were killed within an hour of incubation with 10240 AU mL-1 of its concentrations. Owing to the wide spectrum activity, efforts were made to investigatethe in vitro efficacy of this antibacterial compound in combination with a teat seal formulation, against mastitis pathogens. A new prototype formulation containing the bacteriocin RN 86 with or without minimum concentrations of antibiotics and tween 80, which were the active ingredients of a teat seal used for the treatment of bovine mastitis. The results showed synergistic effect of all three components in combination and were the most effective in controlling the growth of Staphylococcus aureus and Streptococcus agalactiae during in vitro experiments.
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