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Articles by N. Yogananth
Total Records ( 4 ) for N. Yogananth
  N. Yogananth , R. Bhakyaraj , M. Syed Ali and R. Muthezhilan
  Background and Objective: Kaempferol is the important polyphenolic flavonoids that is used to the remedy for cancers, cardiovascular disease, neuron disorder, cholesterol and serve as antioxidant and anti-inflammatory. Materials and Methods: The present investigation was the comparative analysis of kaempferol from field grown plant parts and in vitro callus and also the enhancement of kaempferol by the influence of yeast elicitor were determined. In the present study, at the concentration of 2.0 mg L1 2,4–D+0.5 mg L1 KIN gave the maximum callus growth for both stem and leaf explants (3.47±0.50e and 3.56±0.44e, respectively). Results: The maximum content of kaempferol in three type of samples was observed in this order; yeast elicitor treated samples > in vitro raised callus samples > field grown samples. The highest amount of kaempferol was fount in 1 mL yeast elicitor treated with stem callus sample (0.986 mg g1 dw). Conclusion: Therefore, the present finding it was proved that the highest accumulation of kaempferol in elicitor treated in vitro callus is provides resource for preparation of drugs against various diseases by the pharmaceutical industries.
  N. Yogananth , R. Bhakyaraj , A. Chanthuru and S. Palanivel
  A micropropagation protocol for the important medicinal plant species Dregea volubilis was developed using nodal explants by culturing on Murashige-Skoog (MS) media fortified with different concentration of KIN and BAP. The highest number of multiple shoots (9.36±0.67e) was noticed at the KIN level of 3.0 mg L-1 followed by 8.26±0.62e shoots in KIN 4.0 mg L-1. The in vitro raised micro shoots produced highest percentage of rooting (78%) in MS media augmented with 1.5 mg L-1 IBA. The hardened plantlets were acclimatized in the green house conditions and then reintroduced in the Herbal garden attached to the Centre in which 75% of the plants survived.
  R. Muthezhilan , N. Yogananth , S. Vidhya and S. Jayalakshmi
  The present study deals with the distribution of fungal species in Cuddalore dye industrial waste (Lat 11°42`N; Long 79°46`E) and their dye degrading activity. Totally 13 species under 17 genera were isolated and screened for their decolorization activity against methylene blue, gentian violet, crystal violet, cotton blue, Sudan black, malachite green, methyl red and corbol fushion in mineral salt medium and Czepex-Dox broth. In agar medium, decolorization began with the formation of zone of clearance around the colonies. Aspergillus ochraceus, A. terreus, A. niger, Penicillium citrinum and Fusarium moniliforme decolorized maximum number of dyes to a great extent. Mucor racemosus, Cladosporium cladosporioides, Penicillium oxalicum and Trichoderma viride did not decolorize any of the dyes tested. In liquid medium, decolorizing activity was measured spectrophotometrically. Aspergillus ochraceus, A. terreus, A. niger, Penicillium citrinum and Fusarium moniliforme registered maximum color reduction, where as Mucor racemosus, Cladosporium cladosporioides, Penicillium oxalicum and Trichoderma viride expressed very low amount of color reduction. Biomass and the extent of dye removal are directly propositional. Among the 13 species of fungal isolates, Aspergillus ochraceus, A. terreus, A. niger, Fusarium moniliforme and Penicillium citrinum seems to be potential candidates for dye degradation. These strains can be used for the bioremediation of environs polluted with dye effluents.
  N. Yogananth , M. Akila , M. Syed Ali , M. Jayaprakashvel and R. Muthezhilan
  Background and Objective: Now a days, the need for remarkable disease resistance, growth of aquatic organisms and feed competence has brought about the employ of probiotics in aquaculture field. The objective of the present study was to isolate probiotic bacterial strains from fish intestines and screen them by in vitro testing of their antagonism to pathogens and bioencapsulation efficacy. Materials and Methods: Six bacteria were isolated from marine fish gut and identified by biochemical test. All six isolates were assessed in vitro for its inhibitory activity against common fish pathogens of Aeromonas hydrophila and Vibrio harvey by well diffusion assay. Based on the antimicrobial activity, the Enterococcus, Streptococcus and Bacillus were selected for assaying probiotic properties of acid tolerance, bile salt concentration and auto aggregation assay. The 24 h old Artemia salina nauplii were selected for probiont encapsulation study. Results: The isolates Enterococcus, Streptococcus and Bacillus were confirmed as probionts by above mentioned three methods. The research of encapsulation studies exposed that the gut loading and evacuation time of Artemia nauplii differed according to the oils and probiotics used for the trials. Probiotics take less time for gut loading and retention when compared to oils. Conclusion: The findings of this study indicated that the indigenous fish gut bacteria provided benefit to the culture fishes in terms of pathogen protection and increasing growth potential and thus fulfilled the major requirements of being effective probiotics
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