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Articles by N. Thajuddin
Total Records ( 18 ) for N. Thajuddin
  R. Yang , N. Mohan , C. Muthukumar , N. Thajuddin and M. Gunasekaran
  The influence of cultural conditions that affect GPX production in Candida albicans grown in Lee's medium was investigated. Optimum temperature and pH for GPX activity were 25°C and 7.2, respectively. Substrate specificity for C. albicans. Glutathion peroxidase was in the order of cumene hydroperoxide>t-butyl hydroperoxide> hydrogen peroxide>benzoyl peroxide. Aeration as well as large head space volume enhanced the growth of C. albicans and GPX production. Arabinose and ammonium sulphate significantly increased the GPX synthesis. Among nitrogen sources, polypeptone enhanced both the growth and GPX synthesis. Various cellular activities are regulated by the level of GSH. Therefore, the level of GPX might be used as one of the criteria in developing new drugs against Candida albicans.
  M. Leo Antony , D. Mubarak Ali , E. Baldev , D. Pandiaraj , R. Praveen Kumar and N. Thajuddin
  Fungi present in indoor environment always create serious health risks for the individual dwellers. The present study was focused on the enumeration of fungal contaminants in the newly constructed lab. Totally 8 different fungi were isolated from the indoor environment by using sedimentation culture plate method. The isolated species were identified conventionally, as well as using the sequence homology of internal transcribed spacer regions. This ribosomal non-coding unit sequence based analysis is the most popular locus for species identification and to study the phylogenetic variation. The fungal species isolated and described are already found to be reported as pathogens present in indoor air. Based on the sequences obtained, phylogenetic tree was constructed using both maximum likelihood and distance matrix depicts that the distance method using ClustalW2 offers better resolution and relates the related genera. The study reveals the methodology in fungal taxonomy and also for environmental monitoring of fungi.
  D. Vijayan , K. Manivannan , S. Santhoshkumar , D. Pandiaraj , M. MohamedImran , N. Thajuddin , K. Kala and M.H. Muhammad Ilyas
  The biodiversity study was executed to reveal the microalgal population in the Gundur Lake located in the southern part of Tiruchirappalli in Tamil Nadu, India. The samples were collected from the six chosen sites in the lake as algal mats and plankton samples during March 2014. In total, 81 species were reported which includes 35 species belongs to Chlorophyta, 9 species belongs to Bacillariophyta and 37 species belongs to Cyanophyta. Eight microalgal genera such as Pediastrum, Cosmarium rhophalodia, Microcystis, Chroococcus, Oscillatoria, Scytonema and Gloeotrichia were found predominant in the lake. Physicochemical parameters of the water samples were also analysed to evaluate the algal diversity. The suitable trophic condition and eutrophic nature of the lake favoured the algal dominance and bloom formation.
  A. Nagasathya and N. Thajuddin
  The composition, distribution and biodiversity of cyanobacteria were investigated in relation to salinities of several saltpans of Southeastern coasts of India from Vedharnyam (10 °22` N; 79 °51` E) to Mandapam (9 °17` N; 79 °7` E). Sixty one species belonging to 21 genera and six families were identified. The salinity was ranged from 48 to 185 ppt. Almost all species were found along the range from 48 to 89 ppt., while there was a relative reduction in species composition and biodiversity with salinity of 150 and 185 ppt. The most widespread cyanobacteria were Spirulina subsalsa, S. labyrinthiformis, Oscillatoria willei, O. salina, O. subbrevis, O. laete-virens, Phormidium tenue and Microcoleus chthonoplastes. Aphanothece microscopica, Arthrospira platensis, Dermocarpa leibleiniae, Oscillatoria terebriformis, Oscillatoria acuminata and Synechocystis salina were limited in their distribution to lower salinities. Nostoc sp. was the only heterocystous form recorded in the hypersaline environment.
  D. MubarakAli and N. Thajuddin
  Extension education is an important step for the sustainable development of human welfare based on the socioeconomic and other important circumstances. This education must adapt to technology transfer procedure in terms of lab-to-land conversion without affecting the wild or common procedures. Many factors which affects the dynamic extension education in order to transfer technology in India are found to be poor literacy, communication, economic status, availability and adoptability and private and government sectors. This review papers addresses the factors which affecting extension education agents in technology transfer in India with special reference to lab-to-land (agriculture) phenomena. It has been provided important effective measures and ideas for better technology transfer procedure and sustainable development of human welfare.
  A. Ilavarasi , D. Mubarakali , R. Praveenkumar , E. Baldev and N. Thajuddin
  In recent decades, microalgae have acquired attention from pharmaceuticals to biofuels. The growth and total chorophyll content of three economically important microalgae (Chlorella sp. NTAI01, Monoraphidium sp. NTAI02 and Scenedesmus sp. NTAI03) isolated from fresh water body in five selected culture media on different days of incubation was studied for biomass production. Biomass feedstock has reviewed great interest to be used as an alternative and renewable source of energy. All the three organisms showed varied growth pattern and total chlorophyll content in different culture media. However the growth and total chlorophyll content of Chlorella sp. NTAI01 and Monoraphidium sp. NTAI02 was optimum in Half strength Chu 10 medium. In case of Scenedesmus sp. NTAI03 the growth and total chlorophyll content was found to be significant in Bold’s Basal medium. The Acidified Bold’s Basal medium and BG-11 medium fairly supports the growth of all the three microalgae whereas the Modified Hoagland’s medium does not support the microalgal growth. The optimized growth medium will be used for biomass production for biofuel application.
  K. Kannan , D. Vijayan , D. MubarakAli , R. Praveenkumar , A. Parveez Ahamed and N. Thajuddin
  Azolla-Anabaena symbiosis is one of the vital roles in the field of agriculture and development. Both the associates have mutualistic relationship and work together for carbon and nitrogen fixation to soil. The present study is to investigate the new entity in the relationship between the Azolla and Cyanobacteria. Endophytic and Epiphytic cyanobacterial associates were isolated from different Azolla sp. viz., Azolla pinnata, Azolla caroliana and Azolla filliculoides. The isolates were further characterized by conventional methods and molecular techniques. A novel entity, Westiellopsis was found in the association with Azolla filiculoides comparatively with previously reported. Furthermore, Anabaena variabilis precluded from Azolla filiculoides was produce red color pigmentation. The C-Phycoerythrin content of the isolated cyanobacterial symbiont was measured by absorption and fluorescence emission spectroscopy. The nutritional optimization was done showed two fold increased in pigment production than the control.
  D. Pandiaraj , D. Mubarak Ali , R. Praveen Kumar , S. Ravikumar and N. Thajuddin
  Most of the marine cyanobacteria especially to the order Chroococcales, Oscillatoriales, Nostocales occur ordinarily as planktonic forms. Their taxonomic assignment was based on morphological and cytological characteristics. The genetic variation in the species and strain level morphological and cytological features is not reliable and molecular characterization of cyanobacteria is necessary for better identification. The samples were collected from Thondi and Kattumavadi in Palk Bay region of Tamil Nadu, India. Biodiversity of cyanobacteria were documented, purified and maintained. The two strains, Phormidium chlorinum NTMP01 and Jaaginema psedogeminatum NTMP02 were selected for further molecular characterization based on 16S rDNA sequence in the strain. Evolutionary relationship and secondary structure was constructed with the sequence. The sequences were submitted to GenBank with accession numbers GU812856 and GU812857.
  A. Nagasathya and N. Thajuddin
  The antioxidant property of cyanobacterial isolates was analyzed by physical (bodyweight change and swimming time) and biochemical parameters (superoxidedismutase activity and total reduced glutathione activity) by using swiss mice at Animal House, J.J. College of Arts and Science, Pudukkottai, Tamil Nadu, India. The efficiency of cyanobacterial isolates was determined by comparing with the antioxidant property of SpirulinaPhormidium tenue (KMD 33) possess significant antioxidant property when compared to other cyanobacterial isolates and Spirulina (commercial grade).
  Sabeeha Sultana , Gowri Srinivasa and N. Thajuddin
  An automated system is developed by making use of the concepts present of image processing and pattern recognition, for accurate detection, identification and automatically differentiating the vegetative cells and heterocyst in Nostoc sp. The motto of this study is to identify the object of interest by using few methods such as edge detection, thresholding to remove unrelated objects from the image. Circular hough transform will detect the object with given radius by collecting maximum voting in a given image.
  A. Nagasathya and N. Thajuddin
  The present study is the first detailed investigation of the diversity and distribution of diatoms in relation to the hydrography in the Saltpans of Southern coasts of India starting from Vedharanyam to Mandapam. Totally 52 taxa, both centric and pinnate diatoms were identified in the present investigation. When compared to Palk Strait and Palk Bay regions less diversity of diatoms was observed in the Bay of Bengal region. Highest diversity of diatoms was observed in the 50 ppt. But only 4 taxa namely Achnanthes hauckiana, Cyclotella striata, Pseudonitzschia seriata and Thallassionema eccentrica were observed at higher salinity (150 ppt).
  A. Ilavarasi , D. Pandiaraj , D. MubarakAli , M.H. Mohammed Ilyas and N. Thajuddin
  Microalgal species are known to have pigments in their cellular constitute at the maximum and are valuable bioactive products. In the present study focused was on the evaluation of efficient extraction methods for photosynthetic pigments from microalgal species. They are, Chlorella sp., Acrochaete sp., Phormidium chlorinum, Jaaginema pseudogeminatum and Chroococcus sp. There are four different extraction methods were adopted for active recovery and are economically feasible such as direct extraction, mechanical grinding, heating and preheated solvent method. It was found that mechanical grinding method has extract two fold increased amount than the other methods. Additionally, this methods is inexpensive, less laborious and active extraction. It is suggested that this method could be used for the extraction of photosynthetic pigments from microalgae for pharmaceutical to biotechnological purpose.
  D. MubarakAli , M.I. Mohammed Ershath and N. Thajuddin
  An exploration of the microalgal biodiversity from different epiphytes and substrates of pool water in temple at Tiruchirappalli District was studied. Totally ten epiphytic forms were selected for this investigation. In that, totally 44 species of 30 genera belonging to 3 families of the Chlorophyceae, Cyanophyceae (heterocystous and non-heterocystous) and Bacillariophyceae were identified and recorded. The dominant species in this environment were Cyanobacteria (Chroococcus sp. and Oscillatoria sp., Phormidium sp.), Green algae (Tetradron sp. and Scenedesmus sp.) and Diatom (Fragilaria sp. and Navicula sp.) were documented. The molecular taxonomy of cyanobacteria were also analyzed, in this regards, DNA was extracted; 16S rDNA gene was amplified and sequenced. The evolutionary relationship was found in the epiphytic microalgae by Neighbour-Joining method by construction of phylogenetic tree.
  A. Suresh , R. Praveen Kumar , D. Dhanasekaran and N. Thajuddin
  The systematic study was conducted on the microalgal flora of Western Ghats and other parts of Eastern Ghats revealed a rich wetland algal resource for biotechnological exploration. The present study reveals with the diversity of microalgal flora in the region of Kodaikanal (10°14' N, 77°28' E), Gudalur (9°19'N 77°12'E), Agasthiyar falls (9°58'N, 78°10'E) and Kolli hills (10°12'N, 77°56'E) located in Western and Eastern Ghats of Tamilnadu, India collected in May 2011. In total, 97 species of micro algae belonging to three taxonomic groups were identified, of which 41 species belonging to Cyanophyceae, 38 species from Chlorophyceae and 18 species from Bacillariophyceae. The predominant species in Cyanophyceae were Aphanothece microscopica, Chroococcus minutus, Coelospharium dubium, Hydrococcus rivularism, Oscillatoria princeps, Nostoc muscorum, Nostoc puncteforme, Nostoc commune, Gleotricha gausii, Calothrix braunii, Rivellaria sp., Tolypothrix tenuis, Scytonema schmidtii, whereas in Chlorophyceae, Chlorella sp., Scenedesmus sp., Pediastrum duplex, Cosmarium consperum, Euastrum elagans, Micrasterias americana and in Bacillariophyceae, Navicula hallophyla, Rhophaldia gebrella, Fragellaria intermedia, Pinnularia virdis, Nitzchia palliate. Physicochemical nature of water samples were analyzed and correlated with the total microalgal diversity. Based on the correlation coefficient data, the micro algae showed positive relationship with dissolved oxygen, salinity, nutrients and negative relationship with temperature and turbidity. The species diversity index (H'), Species Richness (SR) and species evenness (J') were calculated and analyzed for microalgal population dynamic variation in the Western and Eastern Ghats.
  D. Dhanasekaran , P. Sivamani , A. Panneerselvam , N. Thajuddin , G. Rajakumar and S. Selvamani
  Twenty six antibiotic producing Streptomyces were isolated from soils of Cuddalore Tamil Nadu, India and were evaluated for their ability to inhibit plant pathogenic Rhizoctonia solani in vitro. It was found that the isolates having pathogenic inhibitory ability and also controls Rhizoctonia solani damping off in tomato in interested field soils. The Streptomyces isolates tested significantly reduced damping off severity in tomato. A variation in their effect on plant disease severity, percentage dead plants and plant biomass in the presence of the pathogen, was observed among the isolates.
  D. Praveen Kumar , R. Thenmozhi , P.D. Anupama , A. Nagasathya , N. Thajuddin and A. Paneerselvam
  The aim of the investigation was to obtain a potential indigenous biocontrol agent against Fusarium oxysporum f.sp. lycopersici on tomato from different agroclimatic zones in India. Wide collection of Trichoderma and Bacillus was conducted from tomato rhizospheric soil in IIVR farm (Varanasi), IIHR farm (Bangalore), IARI farm (Rajendra Nagar) and farm of APHU (T.P. Gudem) by using Trichoderma Specific Media (TSM) and Nutrient Agar (NA). As a result, total 65 strains were cultured and maintained in which 28 were Bacillus and 37 were Trichoderma, respectively. On the basis of in vitro bioassays viz., dual culture and poisoned food technique it was concluded that 3 most prominent Trichoderma isolates viz., DPNST-4, -8 and -29 and four most prominent of DPNSB-2, -11, -18 and -28 from Bacillus were selected.
  A. Nagasathya and N. Thajuddin
  Biological treatment of wastewaters is a growing field of active research mainly by using microalgae including cyanobacteria. Hypersaline cyanobacterium Phormidium tenue KMD33 isolated from the saltpans of Kattumavadi and was maintained in Mn medium with 100 ppt of salinity. P. tenue KMD33 isolate was inoculated in the 250 mL conical flask containing 200 mL of each effluent samples collected from different locations of Paper Mill industry and were maintained in culture room, Department of Microbiology, J.J. College of Arts and Science, Pudukkottai, Tamil Nadu, India under white fluorescent lamp Among the physicochemical parameters studied initially and after 20 days of incubation period with P. tenue KMD33, considerable reduction in the levels of chloride, salinity, alkalinity, calcium, magnesium, nitrite, nitrate, inorganic phosphate, COD, BOD and sulphate and the increasing levels of pH, dissolved oxygen and electrical conductivity were observed. A significant change in the color reduction (from 60-90%) was recorded after 20 days of incubation period. This preliminary result showed that the hypersaline cyanobacterium P. tenue KMD33 is an efficient candidate for the removal of colour from the effluent.
  E. Baldev , D. MubarakAli , R. Shriraman , D. Pandiaraj , N.S. Alharbi and N. Thajuddin
  The present study focused on the extraction of cyanobacterial exopolysaccharides and their utilization as a bioflocculant. A cyanobacterium, Phormidium sp., was chosen based on the production of Extracellular Polymeric Substances (EPS). Later it was identified as Phormidium persicinum by 16S rDNA gene sequence and sequences were deposited in GenBank with accession number KC 859032. EPS was extracted from this strain using standard precipitation method. It was found that EPS production was maximum at late log phase of cyanobacterial growth (20 days). HPLC analysis revealed that presence of sucrose as a major component in the extracted EPS. Interestingly, the extracted EPS was found to be a good bioflocculant even at very low concentration (10 mg L-1). Cyanobacterial based EPS showed potential bioflocculation which can be directly utilized for the water purification and refining processes.
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