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Articles by N. Bahador
Total Records ( 4 ) for N. Bahador
  M. Baserisalehi and N. Bahador
  Susceptibility of thermophilic Campylobacter isolates from environmental samples to antibiotics was studied to investigate relation between occurrence of plasmid and antibiotic resistant character in Campylobacter jejuni. Antimicrobial susceptibility of environmental isolates of Camp. jejuni, Camp. coli and Camp. lari to antibiotics was assessed by disc diffusion method afterward, three strains of Camp. jejuni F44, P41 and W21 isolates were subjected to plasmid isolation and curing. The results on antibiotic susceptibility of Campylobacter isolates by disc diffusion method indicated that, all the isolates of Campylobacter were sensitive to ciprofloxacin and resistant to cefotaxime, cephalexin and ampicillin. In addition, more than 50% of the Camp. jejuni isolates were resistance to chloramphenicol and erythromycin. Plasmids were detected from 60, 50 and 80% of the Camp. jejuni, Camp. coli and Camp. lari isolates, respectively. The cured strain of Camp. jejuni F44 was sensitive to chloramphenicol and resistant to erythromycin. Hence, the most of Campylobacter isolates tested harbored plasmid and probably chloramphenicol resistant marker is plasmid mediated while, erythromycin resistant marker is chromosomally mediated. Therefore, probably gene resistant markers present in the plasmids can be transmitted among campylobacters in the environment and reach the human population by direct contact and via food products of animal origin.
  M. Baserisalehi and N. Bahador
  Nowadays, bioactive compounds produced by bacteria consider a source of new remedy to eliminate antibiotic-resistant bacteria. Therefore, the main purpose of this study was isolation and identification of soil origin Gram-negative bacteria and their evaluation for production of bioactive compounds. In general, three bioactive compounds producing bacteria were isolated from hundred and twenty five soil samples. These strains identified as Pseudomonas aeruginosa, Pseudomonas putida and Comamonas testosteroni based on phenotypic and 16 SrRNA Gene sequencing methods. The bioactive compounds produced by isolated strains were partially purified and their activities evaluated at different pHs and temperatures. Then, their antimicrobial spectrums assessed against different pathogenic microorganisms. The results obtained indicated that the best temperature and pH for activity of the bioactive compounds were 40-50°C and 6.5-7.5, respectively. In addition, bioactive compounds produced by P. aeruginosa and P. putida showed an antimicrobial property against Staphylococcus aureus, Bacillus cereus, Corynebacterium sp. Listeria monocytogens, Klebsiella pneumonia, Shigella dysentriae and Proteus mirabilis; however, E. coli was resistant to them. Although all the bacteria were resistant to the bioactive compound produced by C. testosteroni, Aspergillus niger and Alternaria sp. were sensitive. Overall bioactive compounds produced by Pseudomonas sp. showed activity against some pathogenic bacteria as well as fungi and might be considered an alternative therapeutics not only in pharmaceutical but also as a preservative in food industries.
  M. Baserisalehi , N. Bahador and B.P. Kapadnis
  A total of 455 domestic animals (cow, horse and camel) and poultry from south of Iran were surveyed for fecal carriage of Campylobacter spp. Out of all collected fecal samples, the highest isolation rate of Campylobacter was recorded among poultry (35%), followed by horse (27%) and cow (21%) while, lowest isolation rate was recorded among camel. Of the 85 Campylobacter strains isolated, 76 were classified as catalase positive Campylobacter. Out of them, high frequency of occurrence was belonged to Campy. jejuni. Furthermore, catalase positive Campylobacter spp. were isolated from all the sources of investigation, other than camel. The results obtained from biotyping of the isolates indicated Camp. lari biotype I followed by Camp. jejuni and Camp. coli biotypes I existed in high frequency; while Camp. jejuni biotype II and untypable Campylobacter existed in low frequency. Overall, domestic animals and poultry other than camels are vehicle of Campylobacter in the area of investigation therefore, the people who living in this area may be infected via feces of domestic animals and poultry.
  M. Baserisalehi , N. Bahador and B.P. Kapadnis
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