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Articles by N. Rajendra Goud
Total Records ( 2 ) for N. Rajendra Goud
  K.C. Raju , Ujjwal Neogi , Ruchi Saumya and N. Rajendra Goud
  Keratinases (E.C. are of particular interest because of their action on insoluble keratin substrates and generally on a broad range of protein substrates. The objective of this study is to isolation, screening, purification and determination of the enzymatic activity of extracellular keratinase from dermatophyte Microsporum gypseum. The study clearly indicates the presence of the enzyme keratinases in the dermatophyte Microsporum gypseum. One milliliter of the purified sample contain 80 μg of protein, 1.09 μmole mL-1 60 min enzyme activity and 13.6 μ mole mg-1 60 min specific activity with respect to the unpurified one. The purified and unpurified state of the enzyme was judged by SDS/PAGE. Purified enzyme showed a single band of molecular weight of 33 kDa. Characterization studies showed optimum activity at pH 8 and at 35°C. The enzyme kinetics increased with increased concentration of MgCl2 and decreasedwith increased concentration of ZnCl2. Maximum biomass and keratinase activity were observed from pH 7.0 to 9.0, which agrees with those described for most feather-degrading Bacillus. In this study, the optimum conditions for keratinase synthesis by the Microsporum gypseum were determined, which will be an essential step for the production of adequate amounts for application in research field and other areas.
  G. Kesava Naidu , S.M. Gaddad , C.T. Shivannavar , N. Rajendra Goud , Ujjwal Neogi and Ruchi Saumya
  The main objectives of this research was to isolate Shiga Toxin Producing Escherichia coli (STEC) from diarrheal samples, stool samples from cattle, beef, mutton samples, water and sewage samples collected from different places in Gulbarga region of Karnataka, India and determination of the antibiotic susceptibility patterns of STEC. The highest number of STEC was found in the Sewage sample (14.84%) where as urine sample did not contain any STEC. Among the 2109 sample 65 were confirmed as STEC. The highest (73%) incidence of resistance was recorded against Ampicillin, closely followed by that against streptomycin (70.77%) and cephalexin (69.23%).While only two antibiotics, chloramphenicol (21.54%) and gentamicin (12.3%) recorded comparatively lower incidence of resistance. The multiple antibiotic resistances were most common. More than 98% of the isolates were resistant to two or more antibiotics. Resistances to three (11 isolates), six (10 isolates) and five (9 isolates) antibiotics were most common. Alarming to note that, few isolates (4.61%) are resistant to all the 12 antibiotics tested and 21.5% of the isolates are resistant to ten or more antibiotics.
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