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| Articles
by
N. Khatun |
Total Records (
5 ) for
N. Khatun |
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R.S. Sultana
,
M.A. Bari
,
M.H. Rahman
,
M.M. Rahman
,
N.A. Siddique
and
N. Khatun
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Plantlets regeneration were achieved in callus culture of leaf segments from
five days old field grown seedlings of Citrulus lanatus Thumb. Callus
induction and plant regeneration at various frequencies were observed using
different concentrations and combinations of growth regulators. The highest
percentage of callus induction was observed in MS medium supplemented with 2.5
mg L-1 2, 4-D. After transfer of this callus on MS medium supplemented
with 1.0 mg L-1 BA+0.2 mg L-1 NAA induce adventitious
shoots and developed into plant by further subculture in the same medium. NAA
(0.1 mg L-1) was found effective in the production of root. Plantlets
were acclimatized and subsequently transferred to the field. Survival of the
plantlets under ex vitro condition was 80%. |
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M. Asaduzzaman
,
M.A. Bari
,
M.H. Rahman
,
N. Khatun
,
M.A. Islam
and
M. Rahman
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Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.
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N. Khatun
,
M.A. Bari
,
R. Islam
,
S. Huda
,
N.A.Siddique
,
M.H. Rahman
and
M.U. Mollah
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The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G 12,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G 1 BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G 1 BAP+0.1 mgl G 1IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G 1IBA. Regenerated plants were successfully established in soil after acclimatization. |
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N.A. Siddique
,
M.A. Bari
,
N. Khatun
,
M. Rahman
,
M.H. Rahman
and
S. Huda
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Callus cultures were initiated from axillary shoots on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D, NAA, and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg l G 1 NAA and 2.0 mg l G 1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (86.00%) from axillary shoot derived callus on MS medium containing 1.0 mg l G 1 NAA and 2.5 mg l G 1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil. |
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N.A. Siddique
,
M.A. Bari
,
A.T.M. Naderuzzaman
,
N. Khatun
and
M.H. Rahman
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This paper deals with over one hundred plants of ethnobotanical importance, used by the village doctors especially village kaviraj of Barind Tract for medicinal purposes.This work aims at the preservation and perpetuation of this knowledge of the local plants possessing medicinal properties for the benefit and further fruitful investigation on modern scientific lines and also identification of endangered medicinal plants by questionnaire survey.The data presented in this paper has been collected by personal contact with the local people and herbal practitioners, obtaining the information with great difficulty because of their recticence in divulging the secret of the identity of plants of great traditional reputation. |
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