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Articles by N. Deepthi
Total Records ( 3 ) for N. Deepthi
  N. Deepthi , K.N. Madhusudhan , A.C. Uday Shankar , H. Bhuvanendra Kumar , H.S. Prakash and H.S. Shetty
  The present study was taken up to evaluate the effect of leaf extracts and acetone-precipitated protein of medicinal plants on seed-borne Tobacco Mosaic Virus (TMV) and Tomato Mosaic Virus (ToMV) infection. The antiviral activity was tested on indicator plant Nicotiana glutinosa. Acetone precipitated proteins and solvent extracts of six medicinal plants were tested for their effect on tobamovirus infection. The aqueous leaf extracts of Guava, Phyllanthus and Thuja were effective in reducing the infection by ToMV. The acetone-precipitated fractions of Tridax, Thuja, Guava and Tulsi were effective in reducing the infection by TMV. The solvent extract of Guava was effective in reducing the ToMV infection. Guava extract was subjected to TLC and the fractions were tested for their antiviral activity. Fraction with RF value of 0.014 proved to be effective in reducing the ToMV infection. The solvent extract of Thuja was effective in reducing TMV infection. TLC fraction of Thuja extract with the RF value of 0.12 reduced the TMV infection. Along with this, effect of aqueous leaf extracts on seed quality parameters of tomato and bell pepper was studied.
  N. Deepthi and H.K. Manonmani
  Persistent organic pollutants such as Dichloro diphenyl trichloroethane (DDT) and related compounds are of particular environmental concern because of their toxicity, high persistence, resistance to degradation and liability to bioaccumulation. In our Laboratory, we have developed a defined microbial consortium capable of degrading DDT. The microbial consortium consisted of ten bacterial isolates of which seven were species of Pseudomonas and other three were species of Flavobacterium, Vibrio and Burkholderia. Out of twelve co-substrates used to study the enhancement in DDT-degradation, yeast extract was found to be the best showing 74% degradation at 0.0174 μg mL-1 day-1 followed by glucose and beef extract (55.98%) at 0.0109 and 0.0111 μg mL-1 day-1, respectively. Glycerol and tryptone soya broth showed inhibitory effects with 14.92 and 10.52% degradation with 0.0023 and 0.0015 μg mL-1 day-1, respectively. Growth was best with glycerol followed by peptone. Growth of the consortium was not found to have profound influence on the degradation of DDT.
  N. Deepthi , N.K. Rastogi and H.K. Manonmani
  In our laboratory, a defined microbial consortium was developed and used for the degradation of DDT. The DDT degradation by the consortium was studied for different time intervals. The degradation was maximum in 72 h. DDT-degradation parameters were optimized using Response Surface Methodology (RSM) using this consortium. A Central Composite Rotatable Design (CCRD)with three variables chosen for the study were inoculum concentration (50-1500 μg protein mL-1 i.e., the cells of consortium), temperature (25-35°C) and pH (4-8) each at 5 levels -1.682, -1, 0, 1 and 1.682. Incubation time of 72 h was maintained as constant. Degradation of different concentrations of DDT (5, 10, 20, 30 and 35 ppm) was studied by using this consortium. The optimized conditions were inoculum concentration 1500 μg protein mL-1 and temperature 25°C but pH varied for different concentrations of DDT. Validation of the model was done and experimental values were found to be in agreement with the predicted ones. These conditions can be applied in the degradation of DDT in industrial effluents and other contaminated water bodies.
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