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Articles by Muhammad Farhan
Total Records ( 3 ) for Muhammad Farhan
  Muhammad Farhan , A.U. Khan , Abdul Wahid , Maqsood Ahmad and Farooq Ahmad
  Excessive use of pesticides are disturbing major components of ecosystems. Therefore their removal using biodegradation technique is the need of time. In present study, 35 microbial strains were isolated from industrial drain which carries effluents from chlorpyrifos manufacturing plant. These strains significantly differ in their ability against chlorpyrifos resistance and degradation. Out of these strains WW5 was found most resistant and effective in chlorpyrifos degradation. On the basis of morphological, biochemical and physiological characteristics, strain WW5 was identified as Pseudomonas sp. Biodegradation potential of WW5 strain was studied under different culture conditions like concentration of chlorpyrifos, carbon sources, pH and inoculum densities. In the presence of glucose chlorpyrifos was co-metabolized. High pH (8) and high inoculum density (108 CFU/mL) show most efficient results in biodegradation. Presence of other nutrients enhanced chlorpyrifos degradation probably due to high growth on easily metabolizable compounds which in turn favors biodegradation. The strain WW5 showed 94% degradation of chlorpyrifos (400 mg/L) within 18 days of incubation. This strain can be used for bioremediation and ecological restoration of sites, contaminated with chlorpyrifos.
  Maqsood Ahmad , Amin U. Khan , Abdul Wahid , Zahid Ali Butt , Muhammad Farhan and Farooq Ahmad
  Water samples were collected from 18 sampling sites including the three hospitals their Municipal Wastewater (MWW) drains receiving the hospital sewage and from the river Ravi. The occurrence and fate of ofloxacin (OFLX) and resistance of Vibrio cholerae due to the presence of OFLX was studied. It was found that 16 out of 18 sites were contaminated by the antibiotic. Highest concentration was observed in hospital wastewater (HWW) ranged from 7.31 to 39.13 μg/L and amount of OFLX in the municipal wastewater before mixing the hospital sewage was 0.26 to 0.43 μg/L and after mixing it raised up to 0.54 to 1.29 μg/L. At the entering point of the MWW drains to the river, concentration 0.44 and 0.31 μg/L were observed in the two drains carrying the HWW to the river, respectively. Upstream concentration from the point of entering of the first and second drains was 0.14 and 0.22 μg/L while the downstream concentration was 0.18 and 0.37 μg/L, respectively. The antibiotic was not detected from both the samples collected from the entering point of the river to the city and before entering of the first MWW drain, whereas the after mixing the first drain the concentration 0.043 μg/L was observed. The bacteria isolated from HWW, MWW drains and the river Ravi were 83% 66% and 83% resistant to OFLX, respectively.
  Farooq Ahmad , Saqlain Abbas , Zahid Ali Butt , Adnan Skhawat Ali , Rashid Mahmood , Maqsood Ahmad , Muhammad Farhan , Abdul Wahid , Muhammad Nawaz and Aqsa Iftikhar
  In this study bacterial flora of meat in chicken Shawarma (meat) were investigated from five different regions of Lahore. Samples were taken from internal and external part of Shwarma. The contamination was present in both external and internal part of meat. But external part was found to be little more contaminated as compared to internal part. Analysis of microbes includes E. coli, Salmonella, Aerobes and Coliforms. Microbs were found in order of Aerobes > E. coli > Salmonella there is not too much variation of contamination in different regions but there is variation among the number of bacteria. In every part Aerobes were in greater number as compared to E. coli and Salmonella. Shawarma analyzed from the Site III was more contaminated as compared to other sites. The external part of the product showed more microbial load as compared to internal part.
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