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Articles by Muhammad Rafiq
Total Records ( 3 ) for Muhammad Rafiq
  Mansoor Sana , Asghar Ali , M. Asghar Malik , M.Farrukh Saleem and Muhammad Rafiq
  The field experiment to evaluate the yield potential of seven cultivars of canola (Brassica napus L.) namely Rainbow, Westar, Con11, Dunkeld, Oscar, Shiralee and Defender was carried out at the Agronomic Research Area, University of Agriculture, Faisalabad, during the year 2001-2002. The cultivar Con 11 produced the maximum seed and oil yields due to maximum number of siliquae per plant and seeds per siliqua whereas the cultivar Defender performed poorly and remained at the bottom.
  Mir Saleem Khattak , Fazli Wahab , Javed Iqbal , Muhammad Rafiq and Muhammad Amin
  The study was carried out to propagate olive (Olea europaea L.) cultivars from hardwood cuttings with IBA (Indole butyric acid) at 2000, 4000 and 6000 ppm. IBA at 4000 ppm significantly promoted maximum sprouting (90%), highest survival (76.70%) and produced lengthy shoot (5.8 cm) for cultivar Domate and maximum number of roots 6.9 per cutting in the cultivar at 2000 ppm IBA. Root length in the cultivar was increased at 2000 ppm of IBA. The cultivar N.D. Belice was the 2nd best to give good response to various levels of growth regulator. The cuttings of the remaining cultivars showed little response to IBA.
  Khurram Bashir , Muhammad Rafiq , Tahira Fatima , Tayyab Husnain and Sheikh Riazuddin
  In present studies, level of toxicity of hygromycin was optimized for local inbred line of maize (Zea mays L.). The immature embryos were isolated and cultured on N6 media modified with 1.0 mg L-1 2,4-D, 25 mM L-Proline and 100 mg L-1 Casein Hydrolysate and containing different concentrations of hygromycin. These immature embryos were observed for callus formation on working concentrations of 0, 20, 40, 60, 80, 100 and 120 mg L-1 hygromycin. The results showed that 97.3 38.5 and 32.5% calli survived on media containing 0, 20 and 40 mg L-1 hygromycin. Hygromycin concentration of 60 mg L-1 and above was effective for BR-6 as no callus formation was observed at this concentration or the immature embryos were completely dead. It was also observed that although 32.8% calli survived on media containing 40 mg L-1 hygromycin, these calli were slower in growth, smaller in size and pale in color as compared to calli containing (hph) gene on same media. On the basis of these results 40 mg L-1 hygromycin seems suitable for identification of putative transformants.
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