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Articles by Muhammad Ferhan
Total Records ( 2 ) for Muhammad Ferhan
  Muhammad Ferhan , Zahoor Ahmed , S. Riazuddin , M.I. Rajoka and A. M. Khalid
  Phenol is one of the most common pollutants present in the environment. It enters into the soil and water through different pathways like cooking, plastic manufacturing, oil purification, pharmaceutical and timber industries. Good solubility of phenol in water and its high contents in industrial effluents testify to a high probability of phenol acting as a water pollutant. The industrial waste were collected from (AWT) Army Welfare Trust, Bhi Phareu at different locations and were analyzed spectrophotometrically for phenol concentration by aqueous method. The highest concentration of phenol was observed in the samples. In order to establish bioremedial process of phenolic compounds, bacteria were screened for their ability to utilize phenolic compounds. Pseudomonas putida strain CEMB 10124 degraded and utilized phenolic compounds as carbon and energy source. This strain could degrade 0.8 g l -1 phenol with in 40 h. The growth of bacteria was monitored spectrophotometrically at 600 nm. The disappearance of phenol was scanned between 220-320 nm. The decrease in phenol`s peak at 269 nm depicted the decrease of phenol in the growth medium and it was further analyzed by GC-ECD.
  Muhammad Ferhan
  Experimental bioreactors operated as closed recirculation systems were inoculated with aerobic bacterial cultures that utilized tryptone–yeast extract as carbon and energy sources. These were inoculated with the bacterial culture, which degraded trichloroethylene (TCE) and was observed after 5 days of incubation. Each bioreactor consisted of an expanded bed column through which the liquid phase was recirculated. TCE degradation was also observed with the metabolism of aromatic hydrocarbons established for indigenous microbial population in soil and ground water, in which TCE removal has been shown to be stimulated by the addition of phenol. So co-metabolism occurred when a non-specific enzyme or co-factor was used to transform the growth supporting carbon source, also capable of degrading non-growth supporting compounds. Gas chromatography was use to monitor TCE and their metabolites which compare to run their standards and to check their retention time (tr) values. The retention time (tr) values of phenol, catechol, TCA, TCE were 7.22, 8.82, 8.55 and 2.25.
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