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Articles by Muhammad Azhar Bashir
Total Records ( 2 ) for Muhammad Azhar Bashir
  Muhammad Azhar Bashir , Hamid Rashid and Muhammad Akbar Anjum
  Nodal segments, 1.5-3.0 cm long with 1 or 2 nodes taken from in vitro shoot cultures of six jojoba strains, were cultured on MS medium supplemented with 2.5 or 5.0 mg L-1 BA or 2.5 mg L-1 BA + 2.5 mg L-1 of one of NAA, IAA or IBA. Different growth parameters regarding shoot multiplication were recorded. BA at the rate of 2.5 mg L-1 caused the earliest sprouting of buds and produced the longest primary shoot, maximum number of nodes per primary shoot. However, BA at the rate of 5.0 mg L-1 produced the maximum number of shoots per explant. While the combination consisting of 2.5 mg L-1 BA + 2.5 mg L-1 IBA delayed the bud sprouting and produced the minimum number of shoots, the shortest primary shoot and the minimum number of nodes per primary shoot. Among the strains PKJ-3 led the other strains in performance, while PKJ-2 trailed in all parameters. The number of shoots and the length of primary shoot were significantly affected by the interactions between strains and growth regulators combinations.
  Muhammad Azhar Bashir , Muhammad Akbar Anjum and Hamid Rashid
  The study was conducted to initiate roots from in vitro derived shoots of six promising strains of jojoba i.e., PKJ-1 to PKJ-6. MS medium was supplemented with three auxins i.e., IBA, IAA and NAA each at the rate of 1.25, 2.5 and 5.0 mg L-1 in the one experiment and at the rate of 0.5, 1.0 and 1.5 mg L-1 in the other experiment. Different growth parameters pertaining to root formation were recorded. In the 1st experiment, the lowest concentration (1.25 mg L-1) of each auxin gave satisfactory results, the other two higher concentrations of auxins (2.5 and 5.0 mg L-1) caused callus induction. Efficacy of auxins was improved in the 2nd experiment as each auxin and its concentrations affected significantly the root characteristics of each strain. IBA and 0.5 mg L-1 were the most effective auxin and concentration, respectively. Among the strains, PKJ-3 performed the best of all, as it took the minimum time to root, produced maximum number of roots and attained longer primary root and higher percentage of rooted shoots in both experiments.
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