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Articles by Moulay Sadiki
Total Records ( 4 ) for Moulay Sadiki
  Soumya Elabed , Alae Elabed , Moulay Sadiki , Omar Elfarricha and Saad Ibnsouda
  Background and Objective: The surface physicochemical characteristics play a crucial role in adhesion and biofilm formation. Adhesion process is central to many environmental, industrial and medical applications. Medicinal plants extracts are commonly used in these applications and can potentially influence the bacterium/surface interaction. Two bacterial strains, Staphylococcus aureus CIP54354 and Bacillus subtilis ILP142B and two medicinal plants aqueous extracts types Salvia officinalis and Myrtus communis were examined upon bacterial cell surface physicochemical properties. Methodology: The effect of medicinal plants extracts on bacterial cell surface physicochemical properties was examined using a combination of contact angle measurements, Lifshitz-Van Der Waals (LW) and acid-base (AB) surface free energies calculations. Results: The study demonstrated that plants aqueous extracts treatment could modify cell surface tension parameters including Lifshitz-Van Der Waals (γLW), electron-donor (γ–) and electron-acceptor (γ+) and thereby the bacterial cell hydrophobicity, depending on the aqueous extracts type and concentration and the bacterial surface characteristics. Conclusion: A possible application of these findings in the pharmaceutical industry for the production of compounds supporting antibiotics for treating oral diseases seems to be worth exploring.
  Mounyr Balouiri , Samira Bouhdid , El Houssaine Harki , Moulay Sadiki , Wessal Ouedrhiri , Soumya El Abed and Saad Koraichi Ibnsouda
  Candida albicans is the most common fungal pathogen of humans; this infectious agent can adhere and colonize several surfaces to establish many dangerous infections. The effect of methanol extract from Bacillus sp. isolated from Calotropis procera Ait. rhizosphere on the physicochemical characteristics of Candida albicans cell surface was investigated. The Lifshitz-van der Waals (γLW), acid-base (surface tension components ΔGiwi, electron donor (γ) and electron acceptor (γ+) parameters of the yeast cell surface were assessed using contact angle measurement. Results showed higher antifungal activity of methanol extract against tested yeast. Regarding contact angle measurements, cell surface of control or untreated C. albicans showed a hydrophilic character (ΔGiwi = 19.96 mJ m–2), a strong electron donor character (γ = 48.4 mJ m–2) and a weak electron acceptor character (γ+ = 5.8 mJ m–2). After limited exposure to the antifungal extract, the treated cell surface has become more hydrophilic quantitatively. Moreover, the results showed an increase of the electron donor character and a decrease of the electron acceptor character. However, non-significant modifications on the physicochemical characteristics of cell surface between exposures for 1 and 2 h to the extract were found. The present investigation may provide information that could be used to alter or modify the adherence of C. albicans to biotic and abiotic surfaces.
  Moulay Sadiki , Soumya El Abed , Hassan Barkai , Mounyr Balouiri , Fatima Zahra El Bergadi and Saad Ibnsouda Koraichi
  In this study, the effect of different Thymus vulgaris extract fractions on Cell Surface Hydrophobicity (CSH) and acid-base properties of three fungal strains (Penicillium commune (PDLd10), Penicillium commune (PDLd’’) and Thielavia hyalocarpa (PDLb3)) using contact angle measurement method was investigated. The main results demonstrated that all fractions tested are able to influence fungal cell physicochemical properties. Indeed, the methanolic and ethyl acetate fractions were effective in reducing CSH and made the cells more hydrophilic and highly electron donor (p<0.05) compared to the untreated ones. Whereas, the hexane-ethyl acetate treatment was found made them more hydrophobic and weakly donor electron compared to the control (p<0.05). It is also noted that the modification degree of microbial cell surface properties was fraction-dependent.
  Fadoua Bennouna , Yassir Lekbach , Moulay Sadiki , Soumya El Abed , Saad Ibnsouda Koraichi and Mohammed Lachkar
  Background and Objective: The use of traditional methods as synthetic chemical products to protect cedar wood raises concerns because of the potential negative impact of these products on the public health and environment. The aim of this study was to investigate the chemical composition of three essential oils: Mentha pulegium, Rosmarinus officinalis and Cananga odorata and their antimicrobial effect against six fungi and two bacteria causing degradation of cedar wood. Materials and Methods: Determination of the chemical composition of essential oils was conducted using gas chromatography-mass spectrometry (GC-MS) (Trace GC Ultra). The minimal inhibitory concentrations and minimal bactericidal/fungicidal concentrations were also determined using the broth microdilution assays. Results: The GC/MS analysis of oils studied showed that menthone and pulegone were the major components of Mentha pulegium essential oil, camphene and α-humulene were the major ones in Rosmarinus officinalis, whereas linalool and geranyl acetate were the major components for Cananga odorata. Results also showed potent antifungal activity against all fungi tested, with MICs ranging from 0.0625-0.25% for Mentha pulegium, 0.25-1% for Rosmarinus officinalis and from 0.5-1% for Cananga odorata. An important antibacterial activity was shown against the two bacteria tested with MICs ranging from 0.125-2%. Conclusion: This study suggested that these essential oils may be used as an alternative of traditional methods used for wood protection.
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