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Articles by Mona S. Mahmoud
Total Records ( 3 ) for Mona S. Mahmoud
  M.A. Hassanain , Mona S. Mahmoud and Nawal A. Hassanain
  Serum samples collected from a total number of twenty parasitologically confirmed cases of human fasciolosis were used to evaluate the diagnostic sensitivity and specificity of snail derived antigens; non infected snail (SA), infected snail (ISA), redia (RA), cercaria (CA) and encysted metacercaria (EMCA) of F. gigantica using the enzyme linked immunosorbent assay (ELISA) and enzyme linked immunotransfer blot (EITB). ELISA results showed that the highest level of sensitivity (100%) was with the CA as compared with ISA, RA and EMCA which displayed lower sensitivity levels of 30, 60 and 90%, respectively. All fasciolosis patients were seronegative with SA. Sodium dodecyle sulfate polyacrylamide gel electrophoresis and EITB (with rabbit antiserum raised against F. gigantica somatic antigen) of the snail derived antigens were carried out to characterize their protein profiles and detect cross-reactive polypeptide bands between them. The immunoblot profile of SA displayed cross-reactive bands at 61 and 30 kDa with ISA, RA, CA and EMCA; CA at 61, 34 and 30 kDa with ISA; 96, 61, 34, 30 and 23 kDa with RA and 61, 34, 23 and 19 kDa with EMCA. Cross reactive antigens may be important as possible candidates for vaccine and diagnosis of fasciolosis. Immunoblot of sera from fasciolosis patients using fractionated cercarial antigen on nitrocellulose strips showed that all sera recognized common reactive band at 32.5 kDa molecular weight from cercarial antigen. We suggest that the 32.5 kDa component of Fasciola cercarial antigen may be the most sensitive and specific for the diagnosis of human fasciolosis.
  Nawal A. Hassanain and Mona S. Mahmoud
  Human toxocariasis is one of the most common zoonotic helminthiases. Serological diagnosis remains the main tool for the diagnosis of toxocariasis. Serum samples collected from 57 confirmed cases of human toxocariasis and 26 healthy subjects (negative controls) were tested for anti-Toxocara antibodies by the enzyme-linked immunosorbent assay (ELISA) and enzyme linked immunotransfer blot (EITB) using adult Toxocara canis antigens; somatic antigen (TcSA) and its purified fractions (P-F1, P-F2 and P-F3) and excretory-secretory antigen (TcESA) to evaluate their sensitivity and specificity for the diagnosis of human toxocariasis. ELISA results showed that TcESA is the antigen of choice for diagnosis of human toxocariasis as it showed the highest specificity and sensitivity (100 and 92.30%, respectively). The immunoblot profile of TcESA, TcSA, P-F1, P-F2 and P-F3 reacted with rabbit hyperimmune serum prepared against TcSA showed common immunoreactive bands at 88.00, 85.00, 80.00, 47.462, 40.687, 35.706, 30.527, 21.983 and 19.285 kDa. The immunoblot patterns of them reacted with positive human sera displayed common bands at 47.462, 40.687, 21.983 and 19.285 kDa. The immunoblot profile of TcESA reacted with positive human (58.739, 47.462, 40.687, 30.527, 29.190, 21.983 and 19.285 kDa) and rabbit hyperimmune (94.00, 88.00, 85.00, 80.00, 47.462, 40.687, 35.706, 30.527, 21.983 and 19.285 kDa) sera showed common bands at 47.462, 40.687, 30.527, 21.983 and 19.285 kDa. So, we may suggest that 58.739 and 29.190 kDa polypeptides of the adult T. canis secretory-excretory antigen are specific for Toxocara infection in human and merit further evaluation as candidates for use in the diagnosis of human toxocariasis.
  Mona S. Mahmoud , Sanaa Abou-EL Dobal and Kawther Soliman
  The aim of the present study was to determine and compare the antibody (Ab) response against Fasciola gigantica in experimentally infected rabbits treated with mirazid or carnosine by ELISA. Six groups of five rabbits each, were examined (negative control, negative treated with carnosine, negative treated with mirazid, infected positive control, infected treated with carnosine and infected treated with mirazid). Each insulted rabbit was infected with 20 Fasciola metacercariae. Anti-Fasciola IgG was weekly evaluated by ELISA, in each animal serum, along the period of experiment (14 weeks). After scarifying the all animals, each liver slices were examined for the determination of flukes burden. Results showed highly significant difference between the numbers of Fasciola worms in liver of infected treated and control positive rabbits. A reduction of 54.5% in worm burden was detected in the carnosine treated group. While, the results of mirazid treated group presented complete eradication of flukes. IgG response in experimentally infected and treated groups, either with carnosine or mirazid, was significantly higher than that of control infected group. Carnosine infected group showed the highest Ab response of all groups. However carnosine action was not as potent as mirazid although it raised the general defence of the animal (Ab) plus moderate eradication effect on the worm. Thus, a combination of both carnosine and mirazid might only be recommended in patients presenting other causes of diminished immunity in addition to Fasciola infection.
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