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Articles by Mohsen Amini
Total Records ( 8 ) for Mohsen Amini
  Eshrat Gharaei-Fathabad , Mojtaba Tabatabaei Yazdi , Seyed-Naser Ostad , Shadman Shokravi , Zargham Sepehrizadeh , Mohammad Ali Faramarzi and Mohsen Amini
  A total of 100 supernatants and methanol extract samples obtained from three weeks’ incubation of 50 different isolate strains of cyanobacteria with paddy-fields origin were investigated for their cytotoxicity properties. Fifteen supernatants and 10 methanol extract samples exhibited cytotoxic activity against Balb-C cells while one methanol extract sample possessed a cytotoxic effect against five different cell lines including HeLa, Vero, Caco-2, HepG-2 and CHO more than vincristine, 5-fluorouracil and methotrexate when used at their IC50 concentrations. The later potent extract was achieved from the cyanobacterium strain GT-319. It was characterized as Nostoc piscinale Kützing ex Bornet and Flahault 1888 according to the morphological studies. Identification of the species was performed following molecular taxonomy by 16S rRNA sequencing. This is the first report on the cytotoxic activity of N. piscinale GT-319.
  Mohammad Ali Faramarzi , Mojtaba Tabatabaei Yazdi , Mohsen Amini and Abbas Shafiee
  The whole cells of Acremonium strictum transformed prednisolone at its side chain to produce two steroid compounds. 21,21-Dimethoxy-11β-hydroxypregn-1,4-dien-3,20-dione was the main metabolite which its production has not been previously reported using microbiological means. This metabolite together with a hydroxylated derivative, 11β-hydroxyandrost-1,4-dien-3,17-dione, were purified with preparative TLC followed by their identification through 1H, 13C NMR and other spectroscopic data. Best fermentation condition was found to be 5 day incubation at 25 °C and pH value of 6 according to TLC profiles. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 1 mg mL-1 in one batch. Biotransformation was completely inhibited in a concentration above 5 mg mL-1.
  Abdolhossein Miri , Hamid Reza Monsef-Esfahani , Mohsen Amini , Yaghoub Amanzadeh , Abbas Hadjiakhoondi and Reza Hajiaghaee
  Phytochemical and bioactivity studies of Teucrium persicum Boiss. have been investigated on the aerial parts of the plant. About 2 flavonoids, 5-hydroxy 3, 7, 4-trimethoxyflavone and 5-hydroxy 7, 3′, 4′-trimethoxyflavone were purified and identified from chloroform partition by spectroscopy methods. The crude extracts and isolated compounds were screened for their antioxidant activities using DPPH radical-scavenging, FRAP and Reducing power methods. Methanol extract and isolated flavonoids were found to be the most antioxidant active portions.
  Pirali Hamedani Morteza , Fazeli Mohamad Reza , Samadi Nasrin , Nassireslami Ehsan , Rezazadeh Shams Ali and Mohsen Amini
  In this study the chemical stability of methyl and propyl paraben in magnesium hydroxide suspension (pH about 10) was investigated using both real time (32 months incubation at 25±2°C with humidity of 60±5% RH) and also accelerated (3 months incubation at 40±2°C with humidity of 70±5% RH) methods. Preparation with no added preservative was used as control. Concentrations of methyl and propyl paraben decreased to levels lower than the reported MIC values after the first month and fifth months of real time study, respectively. Preservative effectiveness testing against the Pharmacopeal challanging microorganisms in both suspensions with or without parabens conformed the US Pharmacopeia requirements for oral antacids. It is concluded that alkaline pH of the suspension without parabens could preserve the product against Pharmacopeial challenging microorganisms and incorporation of parabens did not add any antimicrobial activity to the test product.
  Hamid Reza Monsef-Esfahani , Mohammad Ali Faramarzi , Venus Mortezaee , Mohsen Amini and Mohammad Reza Rouini
  A High-Performance Liquid Chromatography (HPLC) method was developed for determination of harmine, harmaline, harmol and harmalol in the extract of Peganum harmala seeds. The sample preparation was performed using liquid-liquid extraction. Chromatographic separation was achieved with a Tracer Excel 120 ODSA (150x4.6 mm) column, using a mixture of potassium phosphate buffer (10 mM, pH 7.0): acetonitrile (100:30; v/v) as mobile phase, in an isocratic mode at 1.5 mL min-1. UV detection (λ = 330 nm) was used. The calibration curves were linear (r2>0.998) in the concentration range of 0.5-20 μg mL-1 for all analytes. The lower limit of quantification for all analytes was 0.5 μg mL-1. The within and between day precisions in the measurement of QC samples at three tested concentrations were in the range of 0.6-10.2% for all analytes. The HPLC method is able to measure the harmala alkaloids in the plant extract. The method has suitable reproducibility, sensitivity and resolution for routine and accurate use with UV detection.
  Hamid R. Monsef-Esfahani , Reza Hajiaghaee , Ahmad R. Shahverdi , Mohammad R . Khorramizadeh and Mohsen Amini
  No phytochemical investigation regarding Scrophularia striata Boiss. (Scrophulariaceae) has been performed, although several reports about other Scrophularia species have been published. The inhibitory effects of aerial parts of S. striata on matrix metalloproteinase expression elaborate a new approach to treat variety of malignant and inflammatory disorders. Five known compounds, including cinnamic acid, three flavonoids (quercetine, isorhamnetin-3-O-rutinoside and nepitrin) and one phenyl propanoid glycoside (acteoside 1) were isolated from S. striata Boiss. by chromatographic techniques and the structures of compounds were characterized by spectroscopic methods. This is the first report regarding the isolation of these compounds from S. striata.
  Hamid Reza Monsef-Esfahani , Abdolhossein Miri , Mohsen Amini , Yaghoub Amanzadeh , Abbas Hadjiakhoondi , Reza Hajiaghaee and Yousef Ajani
  Essential oils from the aerial parts of Teucrium persicum Boiss. (Labiatae), collected in 3 stages (pre-flowering, flowering and post-flowering) from plants grown in the Fars province of Iran were obtained by steam distillation. The chemical composition of the oils were analyzed by GC-MS. Analysis of the chemical profile of the isolated oils revealed the presence of >80 compounds, mainly sesquiterpene hydrocarbons, oxygenated monoterpenes and sesquiterpenes. They contained α-terpinyl acetate, α-cadinene, 1,4-cadinadiene, linalool and cadinol. Average yield was 1%. The studied essential oils showed good antioxidant activities as calculated by 3 in vitro assays: DPPH radical scavenging, Ferric Reducing Power Assay (FRAP) and Reducing Power (RP).
  Hossein Khedmat , Mohsen Amini , Amir Masoud Jafari , Farhad Nasiri Afshar , Mohamad Javad Soltanpoor , Farahnaz Fallahian , Morteza Izadi and Mahboobeh Sadat Hosseini
  A cross-sectional study was carried out at the endoscopy unit of Bagqiyatollah University of Tehran. From February 2004 to June 2006, 305 consecutive dyspeptic patients were evaluated. With regard to standard methods, biopsies were taken and cultured. For each isolates Minimum Inhibitory Concentrations (MIC) for Metronidazole (MTZ), Amoxicillin (AMX), Tetracycline (TC) and Ciprofloxacin (CIP) were determined by Epsilometer test. Considering standard resistance breakpoints, our data was analyzed and resistance rates were reported. One hundred forty nine patients, had a positive culture. The resistance rate in H.P. isolates were as follows: MTZ (60.4%), CIP (40.3%), AMX (15.4%) and TC (10.7%). 82.6% of isolates were resistance to at least one drug. In resistance group: 56, 39 and 5% of isolates displayed single, dual and triple drug resistant pattern, respectively. In the different age groups or sex groups there was no difference (p>0.1) in the antibiotics resistance rates except for TC (p = 0.01) which was more common in older groups (17.1% versus 4.1%). In present study we confronted with markedly high rate of resistance to metronidazole and ciprofloxacin, furthermore other antibiotics displayed significant resistance rate and there was some level of overlap between resistances to these drugs. It seems that in Iran HP eradication is more difficult and demand more concern.
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