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Articles by Mohey A. Hassanain
Total Records ( 5 ) for Mohey A. Hassanain
  Hasan A. Elfadaly , Mohey A. Hassanain , Raafat M. Shaapan , Nawal A. Hassanain and Ashraaf M. Barakat
  Toxoplasma gondii is an opportunistic zoonotic protozoan, distinguish superior brain parasite load in immune-suppressed patients. Corticosteroids are popular anti-inflammatory with immune-suppressive long course, it possible opportunist higher T. gondii brain parasite load and reverts encephalitis in latent infected personals. The present study concerns this concept and preferred for recognize different levels of T. gondii brain parasite load and immunoglobulin titers in both corticosteroids treated and untreated latent infected mice groups. A total number of 70 Swiss-Webster mice (12-18 g) were divided into four groups, the first and second ones are 30 each (infected-untreated and infected-treated group), the third and fourth 5 each (uninfected-untreated and uninfected-treated control). Administration of glucocorticoid (hydrocortisone sodium succinate) at a dose of 50 mg kg–1 (I.M) injection 3 times a week with oral administration of dexamethasone sodium phosphate in dose of 2.5 mg kg–1 day–1 per mouse in drinking water for sequence 2 months. The 103 bradyzoites from mice brain of cystogenic ME-49 strain was used for inducing latent infected mice groups at 30 days before corticosteroids therapy. Serum and brain tissue samples were collected for serological assay and parasite load estimation from sacrificed mice. The results showed significance elevation of average percent of brain parasite load and IgM/IgG titers. All values exceeds higher and parallel to the progression of corticosteroids term in infected treated group than the infected-untreated one. In conclusion, long-term corticosteroids therapy possible opportunist higher T. gondii brain parasite load and induce encephalitis in latent infected murine model, imitate this serious condition in T. gondii infected patients who received corticosteroids therapy.
  Elsayed M. Bayoumy , Sanaa K.A. Abou-El- dobal and Mohey A. Hassanain
  A new approach is chosen to visualize ecosystem health by using parasite bioindicators in Arabian Gulf off Dammam cost. Three hundred and sixty fish specimens belonging to three species of fish were examined parasitological for external and internal metazoan parasites. The seasonal prevalence of the detected parasites was differing from season to another. The highest rate occurred in summer and spring while, the lowest rate was in autumn and winter, respectively. The obtained data showed that Monogeneans prevalence showed highly significant positive correlations with Crustaceans, external parasites, Digeneans. Monogeneans and external parasites prevalence showed highly significant positive correlations with Zn and Se. While, external parasites and Digeneans showed significant positive correlations with Se only. On the other hand, Monogeneans there is antagonist action with Cr and Fe and Ni. Crustaceans showed highly significant positive correlations with Zn and Se. On the other hand, Crustaceans showed highly significant negative correlations with Cr, Fe and Ni. While, digeneans showed a significant negative correlation with Cr. On the same manner, external parasites showed highly significant negative correlations with Cr and Fe.
  Hassan A. Elfadaly , Mohey A. Hassanain , Raafat M. Shaapan , Nawal A. Hassanain and Ashraaf M. Barakat
  Background: The wastages nourished small ruminants and poultry are still free fed on street wastages and possibly exposed to T. gondii oocysts through feces of outdoor shedder cats and they are regarded as high prevalent sources for human toxoplasmosis via their meat containing viable T. gondii tissue cysts. Materials and Methods: A total No. of 859 samples of both blood and their matching tissue were collected from wastages nourished 455 sheep, 237 goats, 124 chickens and 43 ducks respectively from Giza governorate, Egypt. All animals were assayed serologically using Latex Agglutination Test (LAT) as a screen test and the results were confirmed by ELISA. Tissue samples which were identical to seropositive sera were digested and microscopically examined and exposed to DNA confirmation. The microscopic definite bradyzoites containing sera were bio-assayed through intra-peritoneal passage in mice as viability test to determine both LD50 and LD100 for each species isolate. Histopathological examination was done on symptomatic morbid and dead mice. Results: Corresponding to small ruminants and poultry, results of seropositive percentages were 47.5 and 29.3%, total microscopic 30.1 and 32.7%, DNA detection 74.8 and 71.4% and the total percentages of mice viability test 39.4 and 31.3%. In Addition, the total percentages of LD50 were 30.3 and 31.3%, while the LD100 were 9.1 and 0% in small ruminants and poultry respectively. The histopathological examination of inoculated mice signified cyst forming T. gondii in acute and chronic lesions within vital organs. Conclusion: The wastages nourished small ruminants and poultry is of zoonotic impact and significance and must be directed for incriminate this animal feeding pattern and for avoiding consumption under cooked meat of animals or birds.
  Mohey A. Hassanain , Wafaa T. Abbas and Taghreed B. Ibrahim
  Heavy metal pollution represents a serious problem facing many of the aquatic organisms. Lead is one of the most toxic elements found in many industrial effluents which are metabolized inside bodies and can cause skeletal deformities by impairing developmental processes and bone formation. This study was undertaken to determine the LC50 of Pb-acetate and detect the effect of 1/10 LC50 exposure on fingerlings of Nile Tilapia (Oreochromis niloticus). Modern and accurate diagnostic methods were used; Scanning Electron Microscopy (SEM) of gills, plain X-ray and Energy-dispersive X-ray Spectroscopy (EDX) of the spinal column to explain the elemental analysis. The results indicated that the 96 h LC50 of lead acetate was 146.8 mg L-1. SEM showed a distinct degeneration in gill filaments and pavement cells with increasing of mucus secretion after lead exposure. X-ray revealed slight dorsal spinal curvature. EDX analysis of the spinal column of treated fish showed decrease in calcium and phosphorus weight percents, on the other hand Pb appeared in treated fish but not in control ones. Finally this study can spot a light on the dangerous effects of lead toxicity in developing of fish and impairment of their skeletal ossification which may lead to skeletal deformities.
  Nahed H. Ghoneim , Mohey A. Hassanain , Dalia A. Hamza , Raafat M. Shaapan and Sara H. Draz
  Background and Objective: Cryptosporidium species are important zoonotic protozoan parasites that infect the gastrointestinal tract of most vertebrate animals and man. Cryptosporidiosis infection is responsible for numerous outbreaks of diarrheal disease worldwide. This study was planned for prevalence and molecular detection of Cryptosporidium spp., in calves and hospitalized children from different Egyptian governorates (Cairo, Giza and Al-Bahira). Materials and Methods: A total of 253 fecal samples from cattle and buffalo calves <2 months, 2-6 months and <6 months of age and 115 stool samples from children <2 years, 2-6 years and 6-12 years old were screened by modified Ziehl-Neelsen (MZN) staining technique for the detection of Cryptosporidium oocysts followed by molecular characterization using complemented DNA Polymerase Chain Reaction (cPCR). Results: An overall Cryptosporidium spp., infection rates of 30.4 and 33.9% were detected among calves and children, respectively. The highest prevalence (32.7 and 44.4%) was demonstrated in younger calves (<2 months) and children (<2 years), respectively. On the other hand, a lower prevalence (20.0 and 27.0%) was detected in older calves (>6 months) and children (6-12 years), respectively. The prevalence in relation to fecal consistency was higher in diarrheic (39.8 and 41.1%) than in non-diarrheic samples (20.8 and 23.4%) from calves and children, respectively. The PCR analysis of 7 and 6 MZN stain positive calves and children fecal samples, respectively, revealed the expected positive bands at 835 bp for all 6 tested children fecal samples and for only 3 calve fecal samples, while the other 4 were negative PCR for Cryptosporidium spp. Conclusion: The prevalence of Cryptosporidium spp. had a relationship with age and the high infection rate in calves and children can act as a great source of cryptosporidiosis. The obtained data from this study indicates an important public health problem and a potential risk of zoonotic transmission from animal to human beings in Egypt.
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