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Articles by Mohammed A. Alsaif
Total Records ( 4 ) for Mohammed A. Alsaif
  Mohammed A. Alsaif
  The objective of the present study was to investigate the metabolic response to prolonged systemic injury in Wistar rats supplemented with Nigella sativa L. (N. sativa) oil and/or saturated fat. Forty-eight animals were divided in to three dietary groups; Rat-Chow (RC), N. sativa oil diet (NSOD) (Combination of 4% N. sativa oil and 16% butter oil) and saturated fat diet (SFD) (20% butter oil) and diets were supplemented for six weeks. At end of the dietary treatments, each group was divided in two groups as control and trauma. The systemic injury induced with three subcutaneous injections of turpentine (2 mL kg-1) in the dorso-lumber region by keeping 48 h intervals between the injections. TNF-α, IL-6, AST, ALT, ACP, ALP, glucose, triglycerides, cholesterol, albumin and total protein concentrations were estimated in serum by using commercially available kits. Nucleic acid (RNA), GSH and MDA levels were estimated in tissues. Mean body and visceral fat weights were significantly increased in SFD fed animals while it found remain unchanged in NSOD fed rats. Serum enzymes, interleukins and lipids levels were significantly elevated in trauma groups, the least increase was found in NSOD fed rats compared SFD fed injured rats. RNA, total proteins and GSH levels decreased and MDA levels increased significantly in SFD fed traumatized rats compared to NSOD fed animals. The results revealed with histopathological evaluation found protective effect of N. sativa oil against the trauma-induced metabolic changes. In conclusion, N. sativa oil is more beneficial for accidental or post-surgical patients. Further studies are required to find out its effects over chronic HFD exposure along with chemically-induce pro-oxidation process.
  Mohammed A. Alsaif
  The aim of the present study is to determine if a combination of rutin (vitamin-P) and vitamin C has any advantage on antioxidant status in streptozotocin (STZ)-induced diabetes in rats. Oral administration on rutin (100 mg kg-1), vitamin C (200 mg day-1) and their combination (50 and 100 mg kg-1) for 5 weeks on the levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), glucose, insulin, Total Cholesterol (TC) and triglycerides (TG) in normal and STZ-induced diabetic rats were evaluated. Reduced glutathione (GSH), Malondialdehyde (MDA) and superoxide dismutase (SOD) concentrations were estimated in liver. Histopathological changes were screened in liver. Body weight significantly (p<0.001) drops although liver and kidney weights were increased in diabetic rats. Hepatic enzymes (AST, ALT and ALP), cytokines (TNF-α and IL-6) and lipids (TC and TG) were significantly elevated in diabetic rats. Treatments with rutin and vitamin C significantly lowered the elevated values in diabetic rats while it found higher in combined treated group. Liver MDA increased, GSH and SOD levels decreased significantly (p<0.001) in diabetic rats. The treatment with rutin and vitamin C lowered MDA and increased the antioxidant levels to near control values. The results verify the presence of oxidative stress in diabetes and suggest beneficial effects of rutin and vitamin C combinations in combating the oxidative stress in this disease.
  Mohammed A. Alsaif
  The aim of this study was to investigate the effect of N. sativa oil on impaired glucose tolerance and insulin insensitivity induced by high-fat diet and trauma. Three dietary groups were used in this study; Rat-Chow (RC), N. sativa oil diet (Combination 4% N. sativa oil and 16% butter oil) (NSOD) and 20% Butter Oil Diet (BOD). Each group was subdivided in two groups; control and trauma. Diets were supplemented for five consecutive weeks body weight increase per week was calculated. At end of the dietary treatments, single dose (2 mL kg-1 body weight) of turpentine was injected in the dorso-lumber region. Intravenous glucose tolerance test (iv GTT) was performed, insulinogenic index and insulin sensitivity was measured. The results showed butter oil diet significantly increased the body weights and visceral fats compared other two groups, respectively. Fasting glucose levels did not change in trauma induced rats while insulin levels increased significantly and it found highest in butter oil diet fed animals. Impaired glucose tolerance was found sever in BOD fed traumatized rats. N. sativa oil diet protected impaired glucose tolerance and insulin insensitivity induced either via saturated fatty acids or injury. In conclusion, N. sativa oil may be used in post surgery diabetic patients to prevent the long going adverse effects from surgical trauma.
  Mohammed A. Alsaif
  The aim of this study was to examine the possible antinephrotoxic activity of rutin (vitamin P) and vitamin C in kidney of streptozotocin (STZ)-induced diabetic rats. Oral administration on rutin (100 mg/kg), vitamin C (200 mg/day) and their combination (50 and 100 mg/kg) for 5 weeks on body and kidney weights and the levels of serum glucose, insulin and creatinine in normal and STZ-induced diabetic rats were evaluated. Reactive Oxygen Species (ROS), Malondialdehyde (MDA), reduced glutathione (GSH), oxidized glutathione (GSSG) concentrations and Glutathione Peroxide (GPx), Superoxide Dismutase (SOD) and Catalase (CAT) activities were estimated in kidney. Body weights decreased and kidney weights increased significantly (p<0.001) in diabetic rats and those changes were more significantly reduced in the combined treatment group. Abnormal levels of glucose and insulin in diabetic rats were more normalized in the coadministered group. ROS and MDA concentrations were significantly (p<0.001) increased in diabetic rats. The treatments with rutin, vitamin C and their combination to the diabetic rats significantly decreased the elevated ROS and MDA levels in kidney compared to diabetic control rats. The ratio of GSH/GSSG was significantly reduced in diabetic rats and these changes were rectified significantly (p<0.001) by the combined vitamins treatments. GPx and CAT were decreased while SOD was increased in diabetic rats, however, these activities were bring back to normal in combined treated animals. In conclusion, long-term treatment with these vitamins particularly in combination as diabetic maintenance therapy may consequently control or prevent the development of diabetic complications especially diabetic nephropathy.
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