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Articles by Mohammad Y. Alfaifi
Total Records ( 3 ) for Mohammad Y. Alfaifi
  Mohammad Y. Alfaifi , SeragEldin I. Elbehairi , Ali A. Shati , Usama A. Fahmy , Nabil A. Alhakamy and Shadab Md
  Background and Objective: Role of dietary phenolic compounds in the modification of pathophysiological conditions in cancer is immense. Thus, ellagic acid (EA), a natural polyphenolic compound has been recognized for its anticancer activity in different preclinical studies. However, clinical application is limited because of its poor aqueous solubility and thereby, inadequate oral bioavailability. The present work was aimed to formulate micellar delivery, an effective nano-delivery tool for EA, using D-α-tocopheryl polyethylene glycol succinate (TPGS) by film-hydration method. Materials and Methods: Film hydration method was introduced to encapsulate EA within the TPGS micellar structure, which was then characterized and evaluated for in vitro release study. EA-TPGS micelle was further exposed to OVACR3 to determine cancer protecting potential of the formulation. Results: The delivery system (EA-TPGS micelles) consists of spherical shape of 113.2±23 nm with 0.260±0.038 PDI and drug-encapsulation efficiency of 88.67%±3.21. In vitro release profile in the phosphate-buffer saline was found to observe sustained pattern with 67.8% cumulative release within 12 h. Further, a dose dependent cytotoxicity of EA-TPGS micelles was observed on OVACR3 cells with an IC50 value of 12.36 μM. EA-TPGS significantly reduced viable cells via arrest of G1 phase of cell cycle and thereby induce apoptosis probably through inhibiting p15 and p21. Decreased fluorescence unit in ROS determination assay also reflected potential antioxidant activity of the EA-TPGS. Conclusion: These findings strengthened that use of EA-TPGS micelles could overcome the limitations in delivery of hydrophobic chemotherapeutics through oral route.
  Mohammad Y. Alfaifi , Ali A. Shati , Mohammed Ali Alshehri , Serag Eldin I. Elbehairi , Usama A. Fahmy and Ohoud Yahya Alshehri
  Background and Objective: Reports revealed atorvastatin (ATR) exerts significant anti proliferative properties against cancer cell lines. Aim of this study was the potentiation of ATR cytotoxic effects against HepG2 cells by using TPGS as a surfactant and loading on PLGA NPs. Materials and Methods: Nanoprecipitation method was used to trap ATR within PLGA nanoparticles, the method uses vitamin E TPGS as an emulsifier. The nanoparticles’ encapsulation efficiency, size, zeta potential (ZP), surface morphology and in vitro drug release were evaluated. Results: The mean size of the particles was 176.2±14.1 nm, they had a ZP of -15.1±4.4 mV and polydispersity index of 0.32 and after 12 h, approximately 96% of raw ATR had dissolved in comparing with 25% of raw ATR. It was found that ATR-NPs was twice as cytotoxic as the raw ATR. In both instances, for HepG2 cells cycle analysis accumulated at the pre-G1 apoptotic phase in response to ATR and ATR-NPs. Indeed, increase of cells at pre-G1 initiated by ATR 4.45 times the normal level, rising to a 24.05-fold increase for ATR-NPS. The ATR annexin V-FITC apoptosis assay showed significant increase in the amount of annexin V-FITC positive apoptotic cells (both early and late apoptosis) in HepG2 cells treated with ATR-NPs. Conclusion: These findings suggested that this formula is a promising therapy for patients with liver cancer.
  Mohammad Y. Alfaifi
  Anything that poses a challenge or a threat to the well-being is a stress. Understanding the genetic material and cellular response of rats threatened with repeated swim stress provides insights that can influence human healthy. The aim of the present study was to assess the genetical damage and cytological changes caused by exposure of the test organism (Rattus rattus) to forced swim stress. For this purpose, animals have been submerged in water path 15 min daily for 2 weeks. Following that we performed a Micronuclei (MN) test using MNNCE (Micronucleated Normocromatic Erythrocytes) and MNPCE (Micronucleated Polychromatic Erythrocytes), NDI (Nuclear Division Index) and cytological parameters using NDCI (Nuclear Division Cytotoxicity Index), necrotic and apoptotic cells in rat’s bone marrow samples. Results showed that there was a slightly but not significant increase in the frequency of micronucleated as well as in cytological parameters in bone marrow cells. In light of these results if the time-influence taken account forced swim stress may be considered unsafe suffering for a long time.
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