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Articles by Md. Golam Kabir
Total Records ( 2 ) for Md. Golam Kabir
  Md. Monirul Islam , Md. Golam Kabir , A.F.M. Tariqul Islam and Md. Abdur Rakib
  Using monoclonal antibodies (MAbs), the coagglutination test detected Vibrio cholerae O1 and O139 serogroups both in the bacterial culture and in the stool specimens. Detection of V. cholerae O1 and O139 from the cultures of different V. cholerae O1 and O139 strains and non-O1 and non-O139 strains showed a specificity of 100%, a sensitivity of 100% and a 100% positive predictive value. Among the 146 stool samples tested by the coagglutination test, 36 were culture positive, but 33 were coagglutination test positive showing a specificity of about 92%. All the culture negative samples were negative in the coagglutination test indicating a specificity of 100%.
  Md. Monirul Islam , Md. Golam Kabir , Pricila Khan Moly , A.F.M. Tariqul Islam and Md. Abdur Rakib
  Studies were carried out on monoclonal antibodies (MAbs) ICL12, ICL26 and ICL31, which had been produced against Vibrio cholerae O1 and O139. These MAbs of IgG isotypes were used for the development of the Immunomagnetic Separation together with Polymerase Chain Reaction (IMS-PCR). Using MAbs ICL12, ICL26 and ICL31, the IMS-PCR procedure detected V. cholerae. in the pure cultures and in the stool samples. The detection limit of this method was 101 cfu mL-1 for V. cholerae. O139 while for the detection of V. cholerae. O1 Ogawa it was 102 cfu mL-1 and for V. cholerae. O1 Inaba it was 103 cfu mL-1. Of the 25 fresh stool samples tested, 15 were culture positive but 18 were IMS-PCR positive. Of the 55 frozen stool samples tested, 46 were culture positive but only 34 were IMS-PCR positive. However, when the 71 stool samples were frozen with Bovine Serum Albumin (BSA) added subsequently tested, 37 were culture positive while 49 were IMS-PCR positive. This showed that when stools were frozen with added BSA, the IMS-PCR procedure was much more specific and sensitive than the conventional culture method.
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