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Articles by Manisha Srivastava
Total Records ( 4 ) for Manisha Srivastava
  Ravindra Kumar , Seweta Srivastava , Manisha Srivastava and Asha Sinha
  In present study, the influences of different organic soil amendments on soil fungi was studied. Soil organisms carry a wide range of processor that are important for soil health and partially in both natural and managed agricultural scales. The total number of organisms, the diversity of species and activity of soil biota will fluctuate as soil environment changes. Three types of soil amendments and fertilizers viz., urea, FYM and vermicompost were used to amend the cultivated agricultural soil. The fungi were isolated from soil by using dilution plate technique and soil plate method. The dynamics of soil fungi were observed qualitatively as well as quantitatively. The maximum number of fungi was recorded when soil amended with FYM (40.6x104 g-1), urea (38.8x104 g-1) of dry soil at different concentration 2.0, 1.5 and 2.0%, respectively. In control where soil was not amended with any organic amendment, the number of fungi was 13.0x104 to 16.8x104 g-1, 14.4x104 to 16.8x104 g-1, 13.8x104 to 16.8x104 g-1 in urea, FYM and Vermicompost, respectively. A total 25 fungi were observed during the experimental period. Eighteen were observed when soil amended with urea, twenty-two observed when soil amended with FYM and 20 when soil amended with vermicompost. In control soil only fifteen fungi were recorded. The result showed that the number of fungi was increased in amended soil. Qualitatively, the fungi Rhizopus stolonifer, Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Penicillium citrinum, Alternaria alternata and Curvularia lunata, White Sterile Mycelium and Black Sterile Mycelium were observed.
  Ravindra Kumar , Asha Sinha , Seweta Srivastava and Manisha Srivastava
  The aim of the present study was to determine the decomposition rate of Sesbania aculeata L. by CO2 evolution and to estimate Soluble Crude Protein (SCP) production by dominant decomposing mycoflora of Sesbania aculeata L. Eight dominant decomposing mycobiota were selected for the study. In the substrate induced respiration the significant difference was observed in both sterilized and unsterilized substrate with the test fungi. The maximum CO2 evolution was observed with Aspergillus niger in sterilized (16.04 μg day-1) and unsterilized green manure (18.92 μg day-1). In other experiment conducted for the estimation of soluble crude protein production Penicillium citrinum has produced maximum SCP (26.54%) at 25°C followed by Trichoderma harzianum, Aspergillus niger and Curvularia lunata whereas minimum soluble crude protein production was observed in Penicillium rubrum (8.46%) at 35°C. The maximum per cent biomass reduction observed by Aspergillus niger (28.60%) at 25°C and minimum was found in Penicillium rubrum (3.80%) at 35°C. Among seven different nitrogen sources tested against Penicillium citrinum, the highest producer of SCP, potassium nitrate was found to be the best for maximum SCP production (26.54%) whereas the least suitable nitrogen source for SCP production by Penicillium citrinum was recorded to be sodium nitrate (14.85%).
  U.P. Gupta , Manisha Srivastava and Uttam Gupta
  The present investigation was conducted to see the effect of Soybean Mosaic Virus (SMV) infection on carbohydrate content in nodules of soybean. Observation made on the carbohydrate (reducing sugar, non-reducing sugar and starch) content of nodules in soybean mosaic virus infected soybean indicated the reduction of all the contents in comparison to nodules in the healthy plants. Reducing sugar ranged from 2.0 to 3.39 mg g-1 dry weight in healthy and in diseased it ranged from 1.86 to 3.00 mg g-1 dry weight and in non – reducing sugar ranged from 1.4 to 2.75 mg g-1 dry weight and in healthy and in diseased it ranged from 1.3 to 2.45 mg g-1 dry weight. The results were significant in the case of non-reducing sugar. The reducing and non-reducing sugars were estimated by the colorimetric method. The alcohol extract was clarified and residue left on the filter paper was returned to the extracting flask, dried at 65°C and preserved for starch analysis.
  Susheel Kumar Pandey , A.C. Mathur and Manisha Srivastava
  In present study management of chilli leaf curl disease has been studied. The management of leaf curl disease, by plant products showed that Neem Seed kernal extract (5%) found most effective than Karanj and Tumba seed extract. Management by insecticides, imidacloprid 17.8 SL (0.003%) was most effective than spinosad 48 EC (0.02%), malathion 50 EC (0.05%), acephate 75 SP (0.1%) and methyl-demeton 25EC (0.025%). Management of chilli leaf curl was done by seed extract of plants and insecticides at different concentrations.
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