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Articles by Madhuri Behari
Total Records ( 2 ) for Madhuri Behari
  Suhail Rasool , B.L. Jailkhani , M. Irshad , Madhuri Behari , Shahnaz Suhail and H. Shabirul
  High affinity β-bungarotoxin binding protein was purified from skeletal muscle of human cadaver by affinity chromatography using β-bungarotoxin as a ligand. The SDS-PAGE of purified protein revealed two major protein bands with molecular weight of 86 and 68 kD. The purified protein has a kD of 2.3±0.15 nmoles and binding sites of 34±2.3 f mole/mg tissue. Immunoreactivity profile of purified presynaptic receptor (β-bungarotoxin binding protein) from muscle with myasthenic sera was negatively affected by treatment with sodium-metaperiodate, glucosidase and trypsin, where as no effect was seen on treatment with lipase. This provides evidence that purified presynaptic receptor (β-bungarotoxin binding protein) is a glycoprotein.
  Suhail Rasool , Madhuri Behari , M. Irshad , Vinay Goyal and B.L. Jailkhani
  Using α-bungarotoxin (α -Bgtx) and β -bungarotoxin (β -Bgtx) to capture their corresponding proteins from preparation of crude human muscle receptor (triton extract), antibodies against these corresponding proteins were detected in Myasthenia gravis patients. α-Bgtx binds to AChR and β -Bgtx binds to presynaptic membrane receptor (PsmR) of triton extract. The captured proteins were used as antigen in indirect ELISA to detect the IgG antibodies against AChR and PsmR in sera from 146 Indian patients with MG and in 30 controls. Out of 146, 130 were generalised myasthenic patients (GM), 16 ocular Myasthenic patients (OMG). In addition to AChR antibodies, Myasthenia Gravis (MG) patients were also showing antibodies against PsmR. The frequency of anti-AChR antibodies was 71% and anti-PsmR antibodies was 65%. The OD values of GMG for AChR and PsmR were (0.76 ± 0.34) and (0.75 ±0.37), for OMG patients (0.32 ± 0.1) and (0.72 ± 0.42) and for healthy controls (0.16 ±0.06) and (0.18 ±0.04), respectively. These results showed that MG is not only due to the damage of the postsynaptic membrane, but it could concurrently be a consequence of antibody mediated damage of the presynaptic membrane, resulting particularly the damage of its β-Bgtx binding sites and could play an important role in the pathogenesis of MG. Therefore, it is also necessary to detect antibodies against PsmR in addition to AChR antibody.
 
 
 
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