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Articles by M.Z. Hossain
Total Records ( 2 ) for M.Z. Hossain
  M.N. Haque , U.K. Rima , M.Z. Hossain , M.S. Islam , S.M.Z.H. Chowdhury , M.M. Hossain and M.A.H.N.A. Khan
  Objectives: Avian tuberculosis (ATB) is caused by Mycobacterium a. avium, potentially zoonotic and requires adapting molecular techniques to detect pathogenic serovars and prevent zoonosis. Methodology: Layer chicken (N = 2000) of organized poultry farms of Mymensingh district showed clinical signs of progressive emaciation and reduced weight gain constituted the study materials. This study used necropsy, histopathology and Ziehl Neelsen staining to identify specific pathology of ATB in chicken. This study adapted a Polymerase Chain Reaction (PCR) technique to detect ATB in layer chicken due to highly pathogenic variant (serovars 1, 2 and 3) of Mycobacterium a. avium. Results: Investigation of sick birds at necropsy showed granulomas in liver, spleen and intestine and suspected as a case of ATB. Using histopathology, multi-focal accumulation of macrophages, epitheloid cell and lymphocytes were seen in liver, spleen, kidney, heart and intestine. Acid fast bacterium was detected in tissue sections of spleen, liver and intestine using Ziehl Neelsen staining but unable to differentiate infectivity due to pathogenic, low pathogenic and saprophytic variants of Mycobacterium. Visceral organs were, therefore, collected for PCR detection of specific cause of ATB. A specific PCR protocol was adapted targetting 16S rRNA gene (192 bp) and successfully detected pathogenic variant of ATB (M. a. avium) in clinically infected and carrier chickens. Conclusion: The PCR technique showed the potentiality to diagnosis pathogenic variant of ATB in a few hours with high degree of sensitivity and specificity. Pathogenic variant (serovars 1, 2 and 3) of ATB is highly contagious and potentially zoonotic. The PCR technique can be used to screen elderly layer chickens, diagnose ATV at early onset and dispose the infected flock to prevent future zoonosis.
  M.Z. Hossain , S.M.A. Hossain , M.P. Anwar , M.R.A. Sarker and A.A. Mamun
  An experiment was conducted at the Agronomy Field Laboratory of Bangladesh Agricultural University, Mymensingh from July to December, 2001 to study the performance of BRRI Dhan 32 in SRI and conventional methods and their technology mixes. The experiment was laid out in a split-plot design with three replications. The treatments used in the experiment consisted of (A) two planting methods viz., i) SRI planting method and ii) conventional planting method, (B) two types root placement e.g., i) J shape and ii) L shape and (C) three sources of fertilization viz., i) chemical fertilizer (N-60, P2O5-40, K2O-40, S-10, Zn-5 kg ha-1, respectively) ii) organic fertilizer (cowdung, 10 t ha-1) and iii) 50% chemical fertilizer + 50% organic fertilizer. Grain and straw yields were the highest (5.6 and 5.98 t ha-1, respectively) in SRI planting method. The highest grain yield of SRI planting method was mostly the outcome of higher total number of tillers hill-1, highest panicle length and highest number of grains panicle. Conventional planting method produced the lowest grain and straw yields (3.65 and 4.29 t ha-1, respectively). Among root placement methods L shape root placement found to be better and produced higher grain yield (4.97 t ha-1). Fertilization with 50% chemical fertilizer + 50% organic fertilizer performed the best regarding both grain and straw yields. The highest grain yield (5.04 t ha-1) and straw yield (5.67 t ha-1) were produced when 50% chemical + 50% organic were applied while the lowest ones were recorded in chemical fertilizer.

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