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Articles by M.V. Castro-Faria
Total Records ( 2 ) for M.V. Castro-Faria
  L. Lima-Filho , S.R.F. Moreno , R.S. Freitas , S.D. Santos-Filho , M.V. Castro-Faria , G.M.T. Lima , L.C.M. Aleixo and M. Bernardo-Filho
  Phytic acid is a wide-ranging regulator of many important cellular functions such as intracellular regulations of surface receptions channels and it is known to have antioxidant and chelating properties. Stannous fluoride (SnF2) is widely used by the human beings. It has been known for many years that fluoride is an inhibitor of enzymes. In order to analyze whether the sensitive, simple and low cost potentiometric method is capable to inhibit the jack bean urease activity, finally, we evaluated this system specifically to verify the jack bean urease inhibition by phytic acid and stannous fluoride. Inhibition curves showed that stannous fluoride (0.1, 0.5 and 1.0 mM) promoted a 20.34 ? 0.62, 53.13 ? 2.77 and 72.86 ? 4.32 % inhibition of the enzyme, respectively. Phytic acid (10 and 20 mM) did not alter significantly (p<0.05) the enzyme from the inhibition induced by the SnF2. Although, the formation of metal-complexes is a property of phytic acid, our results suggest that the chelating properties of phytic acid, probably, could be responsible by the observed effects. Furthermore, this method can be worthwhile to evaluate the action of some other chemical agents used by the human beings.
  G.L. Lima-Filho , U.C. Araujo , G.M.T. Lima , L.C.M. Aleixo , S.R.F. Moreno , S.D. Santos-Filho , R.S. Freitas , M.V. Castro-Faria and M. Bernardo-Filho
  Copper (Cu2+), a component of the mitochondrial electron transport chain which also functions in iron absorption, mobilization and in maintenance of brain neurotransmitter levels, is well known to generate cytotoxic reactive oxygen species (ROS). The aim of this study was to test a sensitive, simple and low cost enzymatic method based on the urease inhibition by very low concentrations of copper and to use this technique to study the protective effect of phytic acid against copper ions. In this way, an enzymatic method based on the ability of urease to be inhibited by very low concentrations of copper ion was utilized. Inhibition curves showed that copper (0.79 and 1.57 μ M) promoted a 43.82 ± 2.72 and 71.84 ± 6.29% inhibition of the enzyme activity, respectively. Enzyme inhibition by the same copper concentrations in the presence of phytic acid (10 mM) were markedly lower. In conclusion, phytic acid has a copper chellating capacity that may be useful in preventing copper interaction with biomolecules and, accordingly, in lowering the generation of ROS.
 
 
 
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