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Articles by M.R. Nassiry
Total Records ( 4 ) for M.R. Nassiry
  M.R. Nassiry , A. Javanmard and Reza Tohidi
  Problem statement: A wide range of studies for the assessment of genetic diversity in livestock breed were conducted using genetic distance. For high-accuracy and unbiased estimation sampling methods, criteria of choosing type of DNA markers, distance measurement strategies, cluster analysis will be important for any genetic diversity projects. Approach: Main objective of this short review is focusing on application statistical procedures and methods in analysis of genetic diversity data in animals. Results: There is no simple strategy to address for best and effectively genetic diversity results by the way regarding to some important factors can make reliable results for next analysis. Conclusion: There is still a distinct need for developing comprehensive and user-friendly statistical packages that facilitate an integrated analysis of different data sets for generating reliable information about genetic relationships, genome diversity, and favorable allele variation. Equally important and perhaps more challenging, is the concerted and planned utilization of genome information in animal breeding programs on the basis of knowledge accrued from studies on genetic diversity.
  S. Ghazanfari , H. Kermanshahi , M.R. Nassiry , A. Golian , A.R.H. Moussavi and A. Salehi
  The aim of this study was to investigate the effects of feed restriction and different energy and protein contents of the diet on performance and growth hormone concentration in broiler chicken. Five hundred and seventy six day old Ross male broiler chicks were used in a 2x2x3 factorial arrangements in a completely randomized design experiment. Feeding programs consisted of ad libitum and Skip-a-Day (SAD) feed restriction, two energy levels (3100 and 2800 kcal ME kg-1) and three protein levels (22.3, 19.3 and 16.3% CP). Feed restriction (SAD) was applied during 22-32 d of age. Corn-soybean meal based diets containing vegetable oil were used. Body weight and feed intake were recorded weekly. At 21, 32 and 49 day of age, one bird from four replicate of each treatment was selected randomly to collect blood sample and then carcass, breast and thigh weight were measured. Blood samples assayed for Growth Hormone (GH) concentration by RIA. Feed restriction decreased feed intake and body weight gain (p<0.001) of birds during 22-32 and feed intake during 32- 49 day of age, while body weight gain was not affected during this period. Also, feed restriction decreased Feed Conversion Ratio (FCR) (p<0.01) and body weight gain (p<0.001) during 22-32 day of age. Feed intake (p<0.001) and body weight gain increased in broilers fed on low-energy diets compared with those fed on high-energy diets during different periods. Increasing levels of protein increased feed intake (p<0.001), body weight gain and improved FCR (p<0.001) as compared with least level of protein. Feed restriction decreased carcass percentage (p<0.001) and increased thigh percentage (p<0.01) at 32 day of age. Carcass percentage (P<0.05) and breast percentage increased in broilers fed on low-energy diets compared with those fed on high-energy diets during different periods. The low protein diet decreased carcass percentage (p<0.01), breast percentage (p<0.001) during different periods and thigh percentage (p<0.05) at 21 day of age. The result of this experiment indicated that the lowest protein level had the highest growth hormone concentration at 49 day of age. The low energy diet increased growth hormone concentration (p<0.05) at 21 day of age.
  G. Elyasi , J. Shodja , M.R. Nassiry , A. Tahmasebi , O. Pirahary and A. Javanmard
  β-lactoglobulin is the major milk whey protein in ruminants and its coding gene located on ovine chromosome 3. This protein produces in mammary glands during pregnancy and lactation stages. Studies have shown that the protein is polymorphic in many breeds of sheep. This is the result of single base pair substitution in β-lactoglobulin gene that also rises to RsaI restriction Fragment Length Polymorphism (RFLP). Blood samples were supplied from 142 animals of Iranian sheep breeds (Ghezel, Afshary, Moghani, Makoii and Arkharmerino). Genomic DNA was extracted from 100 μL blood sample according to Boom method modified by Shaikhayev. Gel monitoring and spectrophotometeric methods were used for determination of DNA quality and quantity. BLg 5 and 3 primers amplified a 452 bp fragment from exon II of ovine β-lactoglobulin gene. Products of amplification were recognized by electrophoresis on 1% agarose gel stained with ethidium bromide. RsaI enzyme was used for restriction of PCR products. Digestion products were separated by electrophoresis on 8% non-denaturant polyacrylamide gel and visualized after staining with ethidium bromide on UV gel documentation. PopGen 32 software (ver. 1.31) was used to estimating the frequency of allele, genotype and Hardy-Weinberg equilibrium. Frequency of A-allele in Ghezel, Afshary, Moghani, Makoii and Arkharmerino sheep was 56, 34, 36, 53 and 48%, respectively. The populations were in Hardy-Weinberg equilibrium except of Afshary breed.
  N. Asadzadeh , A. Javanmard and M.R. Nassiry
  Clean, high molecular weight DNA is pre-requisite for DNA markers. The amount and quality of DNA is a crucial point for all further analysis. A unique advantage of these PCR techniques is the rapid DNA analysis of many animal samples using small quantities of DNA. Thus, a simple and rapid DNA extraction method is needed for studies such as genetic analysis that require large populations. Several methods for minimizing the DNA extraction steps have been reported but they require a large amount of animal tissues. In addition, bleeding and management of sampling and storage of the blood sample in freezers is often difficult due to space constraints. To overcome these problems, some techniques developed a DNA extraction method using the milk or hair root or semen. Researchers compared 4 methods of rapid DNA extraction with isolations of mammalian whole blood samples. DNA extraction methods included boiling, salting out, phenol-chloroform and silica gel procedures. Spectrophotometry and gel monitoring evaluated the DNA yield and purity for the 4 methods. The silica gel and phenol-chloroform methods yielded significantly purity and higher concentration of extracted DNA compared with other DNA extraction methods.
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