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Articles by M.R. Mehrabi
Total Records ( 8 ) for M.R. Mehrabi
  A. Akbarzadeh , D. Noruzian , SH. Jamshidi , A. Farhangi , M.R. Mehrabi , B. Lame Rad , M. Mofidian and A. Allahverdi
  Induction of experimental diabetes mellitus is indeed, the first step in the process of purification of pancreatic Langerhans islet cells of normal rats for transplantation under the testis subcutaneous of experimentally induced diabetic rats. For induction of experimental diabetes in male adult rats weighting 250-300 g (75-90 days), 60 mg kg-1 of streptozotocin was injected intravenously. Biopsy of pancreas tissue of diabetic and normal rats showed that the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In the process of purification of islet cells, after collagenase digestion of pancreases, islets were isolated and dissociated; employing enzymes like DNase and trypsine, so the islet cells were changed into single cells and these cells were assayed by flow cytometry. Flow cytometry of these cells indicated that there were 91% of beta cells in cell suspension. Donor tissue in each step of this research was prepared from 38 adult wistar male rats. Transplantation was done in rats after 2-4 weeks induction of diabetes. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression. This technique which is called immunoisolation is based on the principle that transplanted tissue is protected for the host immune system by an artificial or natural membrane. The diabetic, treated and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured. Analysis of variance showed a high significant difference between them.
  A. Akbarzadeh , D. Norouzian , A. Farhangi , M.R. Mehrabi , M. Bakhtiari , P. Afshar , A. Allahverdi , M.A. Mofidian and A. Shayan
  For the economic purpose of clinical trial and study of safety and immunogenicity of therapeutic morphine vaccine, 102 out of 200 outpatient volunteer addicts, whom were interested in abstinence, were injected with morphine vaccine, by randomized double blind method and under placebo control. The volunteers were divided into 3 cohorts, each consists of 30 subjects. The cohorts 1, 2 and 3 were injected with 12.5, 100 and 600 μg mL-1 of morphine vaccine, respectively. In each cohort, four additional subjects were injected with placebo. All the volunteers were bled prior to each injection and they got intra deltoid injections at 0-30-60 days and were monitored for safety and antibody production, for 12 months. All of 102 volunteers completed the course of three injections and all of them returned for the final scheduled visit at day 90th. The rise of antibody against morphine in all three vaccinated cohorts was controlled along the 5, 7, 9, 11 and 12 months. The vaccine was well tolerated with dose related increases in antibody levels and had no serious drug-related adverse events. Only 5 persons at the highest dose experienced brief post injection twitching. Anti-morphine antibody was detected by ELISA method after the first injection of 100, 600 μg mL-1 and second injection of 12.5 μg mL-1 doses and reached to its peak in 3 months and did not decline to baseline after one year. Thus, vaccine was well tolerated with dose related increases in antibody levels and a high proportion of outpatient volunteer addicts were recovered.
  A. Goudarzi , M.R. Mehrabi and K. Goudarzi
  The aim of this study was to evaluate the effects of iron deficiency on intelligence of 11-17 years students. This study conducted on the 540 students (11-17 years) that educated at guidance and high school of Boroujerd city. Laboratory investigations were included serum iron, TIBC (total iron binding capacity) and ferretin. Riven matrix was used in order to determine intelligence quotient. Data were analyzed using SPSS 13 and χ 2 and t-tests. Results showed that 78 (14.4%) students had iron deficiency and 14 (25.9%) had iron deficiency anemia. There was no significant difference between different sexes for iron deficiency distribution (p>0.05), while iron deficiency anemia was significantly higher in girls as compared with boys (p>0.05). Mean quotient was 115 ±12.1 in iron deficiency students, while it was 113.7 ±13.9 in patients without iron deficiency. There was also no significant difference between normal and iron deficient students for intelligence quotient (p>0.05).
  S. Cheraghi , A. Akbarzade , A. Farhangi , M. Chiani , Z. Saffari , S. Ghassemi , H. Rastegari and M.R. Mehrabi
  The aim of this study is over-expression of Meso-diaminopimelate decarboxylase enzyme (EC and enhancement of L-lysine production rate. The C. glutamicum LysA gene which encodes a Meso-diaminopimelate decarboxylase was cloned in E. coli. The cloned gene was sequenced; it encodes a 445 amino acids protein with molecular weight of 47 kD. Expression of the LysA gene in E. coli resulted in an increase in Meso-diaminopimelate decarboxylase activity, correlated with the presence in sodium dodecyl sulfate-polyacrylamid gels of a clear protein band that corresponds to this enzyme. The induction of cloned gene by IPTG has been shown to have an inhibitory effect on cell growth due to over-expression of the cloned gene. A two fold increase in lysine production rate was observed after introduction of the cloned gene into E. coli.
  M. Chiani , A. Akbarzadeh , A. Farhangi , M. Mazinani , Z. Saffari , K. Emadzadeh and M.R. Mehrabi
  The aim of this study was optimization of culture medium in direction of increasing the production rate of desferrioxamine B. Streptomycetes are the most widely studied and well known genus of the actinomycete family. Streptomycetes usually inhabit soil and are important decomposers. The genus Streptomyces are Gram-positive and GC rich bacteria that are important for production of many antibiotics and secondary metabolites. These metabolites are important in industrial and medical fields. Deferoxamines (also known as desferrioxamine B, desferoxamine B, DFO-B, DFOA, DFB or desferal) are low-molecular-weight, iron-chelating compounds (siderophores) produced and secreted by many actinomycetes, including species of Streptomyces, Nocardia and Micromonospora. Streptomyces pilosus synthesizes the siderofore desferrioxamine B. Desferrioxamine B is used clinically to treat disorders related to iron overload and pathological iron deposition in human. Our results revealed that the use of soybean as a base medium plus additives such as Na2HPO4.12H2O, NaH2PO4, MgSO4.7H2O, ZnSO4.7H2O, FeSO4.7H2O, CaCl2.2H2O, NaCl, MnSO4, NH4Cl, KH2PO4, K2HPO4, some of the amino acids and vitamins increased the production of desferrioxamine B about 8 times in comparison with the control.
  A. Farhangi , A. Akbarzadeh , M.R. Mehrabi , M. Chiani , Z. Saffari , S. Ghassemi , M. Kheiri and R. Bashar
  One of the sensitive and standard tests to control the safety of a vaccine is the inoculation of such vaccine to the air pocket of Lohmann specific pathogen free eggs. The aim of this study is to control the safety of morphine vaccine. This study reveals the safety of morphine vaccine by employing Lohmann specific pathogen free embryo eggs. The changeable parameters in this test were: weight of eggs, safety of eggs embryo, vaccine concentration, normal saline and temperature of the incubator. To study, the safety of morphine vaccine, we used 30 eggs (after controlling the safety of eggs and their embryos) which were divided into two groups of control (15 eggs) and test (15 eggs). After weighing the eggs, the eggs under experiment were inoculated with morphine vaccine and the control group was inoculated with physiological solution. Both groups were incubated and weight of the eggs and chickens were determined accordingly. The eggs of each group were controlled by their weights showing healthy, normal growth and evolution. The comparison between the weights of control and experimental groups did not show any significant changes. Exactly growth and evolution of each group were found equally to be balancing for three weeks after injection. Finally all eggs were observed to be safe, alive and in evolutionary form. By comparing the growth and evolution amongst each egg in the group under experiment, after injection, the eggs did not show any adverse reaction after inoculation with therapeutic human morphine vaccine.
  M. Chiani , A. Akbarzadeh , A. Farhangi and M.R. Mehrabi
  The aim of this study was evaluation of corn steep liquor as an alternative or substitution medium for production of desferrioxamine B in streptomyces pilosus. Desferrioxamine B is the major siderophore of Streptomyces pilosus. The genus Streptomyces are Gram positive and GC (Guanine/Cytosine) rich bacteria that are important for production of many antibiotics and secondary metabolites. These metabolites get more attention in industrial and medical fields. Desferrioxamine B is used clinically to treat disorders related to iron overload and pathological iron deposition in human. Corn Steep Liquor (CSL) is a by-product of corn wet-milling. It is an excellent source of organic nitrogen and important constituent of some culture media. Nowadays CSL was mainly used for feeding of livestock. In this study we substitute the conventional media with CSL and surveyed its effect on production of desferrioxamine B. The CSL is cheaper than other media and its availability is so easy. In this research, for the first time we used a cheap material for production of a valuable product. Our results showed that the use of CSL solely, increased the production of Desferal more than three times in comparison with conventional media such as soybean.
  D. Norouzian , A. Farhangi , S. Tolooei , Z. Saffari , M.R. Mehrabi , M. Chiani , S. Ghassemi , M. Farahnak and A. Akbarzadeh
  Bacterial Celluloses (BC) are gaining importance in research and commerce due to numerous factors affecting the bacterial cellulose characteristics and application in different industries. The aim of the present study was to produce bacterial cellulose in different media using different cultivation vessels. Bacterial cellulose was produced by static cultivation of Glucanacetobacter xylinum ATCC 10245 in different culture media such as Brain Heart Agar, Luria Bertani Agar /Broth, Brain Heart Infusion, Hestrin-Schramm and medium no. 125. Cultivation of bacterium was conducted in various culture vessels with different surface area. The cellulose membrane was treated and purified with a 0.1 M NaOH solution at 90°C for 30 min and dried by a freeze- drier at -40°C to obtain BC. The prepared bacterial cellulose was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD). The amount of produced BC was related directly to the surface area of culture vessels.
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