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Articles by M.R. Asran
Total Records ( 2 ) for M.R. Asran
  M.R. Asran and M.I. Eraky Amal
  Fusarium fungi, including F. graminearum, cause seedling blight, foot rot and head blight diseases in wheat resulting in yield loss. Trichothecene mycotoxins are a group of toxic fungal secondary metabolites. This group of toxins is found associated with Fusarium diseases in wheat in numerous countries worldwide. Five isolates of F. graminearum were examined for their degree of disease severity (virulence) and trichothecene production on Sakha 69 wheat cultivar. In greenhouse experiments, following soil infestation all isolates caused pre and post-emergence death of wheat seedlings. Dry weight of infected seedlings was reduced as compared with uninoculated control seedlings. The F. graminearum isolates resulted in varying degree of disease severities in seedlings. All tested isolates caused seedling blight symptoms. However, they differed in their degree of pathogenicity. The reaction of wheat cultivars against seedling blight varied. While cv. Sakha 69 was the most susceptible (35.22%), cv. Giza 164 exhibited the highest level of resistance (14.61%) and cv. Giza 168 showed a moderate degree of resistance (23.17%). The F. graminearum isolates were examined with regard to possible relation between seedling blight severity and trichothecene production. The trichothecenes contents were detected by using gas chromatography equipped with an Electron Capture Detector (GC-ECD). The amount of trichothecenes produced by the various isolates on autoclaved oat grains ranged from 1393-57081 μg kg-1 ground grain. All isolates produced trichothecene in vitro but differed significantly in their level of production. The highest amounts of total trichothecene were detected in grains inoculated with isolate Fg 4.3 (57081 μg kg-1 ground grain) while grains inoculated with isolate Fg 33 and 18.7 had the lowest amounts of total trichothecenes. There was a close relationship between the degree of disease severity and trichothecene concentration.
  F.M. Ibtesam Badawy , M.A. Nashwa Sallam , A.R. Ibrahim and M.R. Asran
  The effect of lime, thyme, comphore oils against Penicillium digitatum, the causes of green mould disease of orange fruits, was evaluated for their inhibitory effect in vitro and in vivo during storage conditions. In vitro experiments, different concentration of each essential oil at 1, 5 and 10% (v/v) was tested on the growth of P. digitatum. The best concentration at 10% showed the highest inhibition growth of P. digitatum for all tested oils. In vivo experiments, in 2009 and 2010 seasons, treating fruits 15 days before harvest and after harvest or only after harvest (natural and artificial infection of fruits) by essential oils at conc. of 10% (v/v) significantly reduced the disease severity of fruits compared with untreated fruits (control) at 5°C. There was no significant difference found between the two tested methods of applying oils on fruits (natural and artificial infection of fruits). All treatments significantly reduced the undesirable fruits percentage, fruit weight loss percentage during cooling storage for 14 weeks compared with control during the two investigated seasons. Also preceding harvest and post harvest spraying with lime essential oil at conc. of 10% caused significant increase in TSS% during cold storage for 14 weeks compared with control. Prolonged cooling storage at 5°C for nine weeks slightly increased total soluble solids percentage of both controls and decline towards the end of storage period (14 weeks) of both treatments and control. At the end of storage period, there was no significant difference on Total Acidity (TA) between all tested treatments and controls. Vitamin C was gradually decreased as storage prolonged, for both control and all treatments.
 
 
 
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