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Articles by M.N. Siti Azizah
Total Records ( 3 ) for M.N. Siti Azizah
  Salman A. Al-Shami , M.N. Siti Azizah , Che Salmah Md Rawi and Abu Hassan Ahmad
  Problem statement: Chironomidae have been recorded in rice fields throughout the world including in many countries such as India, Australia and the USA. Although some studies provide the key to genera level and note the difficulty of identifying the larvae to species level. Chironomid researches have been hindered because of difficulties in specimen preparation, identification, morphology and literature. Systematics, phylogenetics and taxonomic studies of insects developed quickly with emergence of molecular techniques. These techniques provide an effective tool toward more accurate identification of ambiguous chironomid species. Approach: Samples of chironomids larvae were collected from rice plots at Bukit Merah Agricultural Experimental Station (BMAES), Penang, Malaysia. A 710 bp fragment of mitochondrial gene Cytochrome Oxidase subunit I (COI) was amplified and sequenced. Results: Five species of Chironomidae; three species of subfamily Chironominae, Chironomus kiiensis, Polypedilum trigonus, Tanytarsus formosanus, two species of subfamily Tanypodinae, Clinotanypus sp and Tanypus punctipennis were morphologically identified. The phylogenetic relationship among these species was been investigated. High sequence divergence was observed between two individuals of the presumed C. kiiensis and it is suggested that more than one species may be present. However the intraspecific sequence divergence was lower between the other species of Tanypodinae subfamily. Interestingly, Tanytarsus formosanus showed close phylogenetic relationship to Tanypodinae species and this presumably reflect co-evolutionary traits of different subfamilies. Conclusion: The sequence of the mtDNA cytochrome oxidase subunit I gene has proven useful to investigate the phylogenetic relationship among the ambiguous species of chironomids.
  Z.A. Muchlisin and M.N. Siti Azizah
  Hassan Ghadirnejad , M.N. Siti Azizah , Aliakbar Salehi , Kamran Aghili , Katialisa Kamaruddin , A.F.J. Jamsari and Lim Hong Chiun
  The universal barcoding gene of Cytochrome Oxidase I (COI) was utilised for molecular characterisation of five sturgeon species found in the Iranian waters of the Caspian Sea. These were Acipenser gueldenstaedtii (Russian sturgeon), A. persicus (Persian sturgeon), A. stellatus (Stellate sturgeon), A. nudiventris (Spiny sturgeon) and Huso huso (Great sturgeon). An additional 10 sequences from the Genbank were included in the analysis, A. stellatus (AJ585050), H. huso (AY442351) A. baerii (FJ205560-FJ205563), A. fulvescens (EU524397), A. transmontanus (EU523891), A. medirostris (EU523885) and A. oxynrinchus (EU524401). The data was also analysed for phylogenetic insights among the studied species. The results confirmed the utility of this gene for molecular identification and revealed monophyly of three of the species; H. huso, A. nudiventris and A. stellatus. However, the status of A. gueldenstaedtii and A. persicus was unresolved with Genbank sequences of A. baerii (FJ205560-FJ205563) also clustering in the A. gueldenstaedtii-persicus complex as had been reported by previous researchers investigating other genes. Thus, the results provide further evidence of the polyphylic` nature of A. gueldenstaedtii. The data is also in agreement with several earlier reports that H. huso may not be a separate genus from Acipenser within the family Acipenserinae. The low internode bootstrap values at branch nodes did not allow for any phylogenetic inference among the Acipenser species to be deduced.
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