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Articles by M.M. Mukhtar
Total Records ( 7 ) for M.M. Mukhtar
  M. Haroun , I. Hajer , M.M. Mukhtar , S.A.M. Kheir and B.E. Ali
  In vitro cultivation of rinderpest virus is a routine procedure to isolate the virus in B95a cell lines. Monocyte modified human cell lines (MoMo) were not known to be used commonly for cultivation of Rinderpest Virus (RPV). In this study the ability of both cell lines to support replication of RPV was tested. B95a and MoMo cell lines sustained replication of RPV-Saudi 1/81 virulent strain. Classical RPV Cytopathic Effect (CPE) was observed in both cell lines starting at day 5-6 of co- cultivation with Peripheral Mononuclear Cells (PBMC) of the infected calves. RPV P-protein antigen was detected in both infected cell lines on day 4 of inoculation.
  Layla Mohamed and M.M. Mukhtar
  The viability, Purity and count of monocytes isolated from peripheral blood of camels were assessed after the isolation of these cells on 9, 10 and 12% concentrations of Ficoll/Hypaque. The results obtained showed that 97% viable cells were obtained using the three concentrations of the Ficoll/Hypaque. For purity, 12% Ficoll concentration separated cells were contaminated with others blood cells, while 9 and 10% separated cells were pure. When cells count was applied for the pure isolated cells, the cells isolated on 10% Ficoll/Hypaque concentration showed the best highest count compared to 9% with p = 0.003. Cells isolated using 10% Ficoll/Hypaque well proliferated when stimulated with Phytohaemaggglutnin (PHA) mitogen.
  Abbas Mohamed Ahmed , A.M. Zakia , M.M. Mukhtar and A.M. El-Hussein
  Skin sections of sheep inoculated with live spores anthrax vaccine revealed edema, intense effusion of mononuclear cells and proliferation fibroblast in the dermis and subcutaneous tissues, whereas sections of skin of those inoculated with capripox vaccine showed hyperplasia and hydrophopic degeneration of epidermal epithelium and instance infilteration of cellular exudates in dermis and subcutaneous tissues. However, the lesions presented by the combined vaccine included the fore mentioned lesions.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nourand and M.A. Fadol
  An anthrax and sheep pox combined vaccine was made in a lyophilized form by mixing concentrated live spores of Bacillus anthracis (Sterne strain) of known count with sheep pox virus (0240 vaccine strain) of a known titer. It induced protection against anthrax in guinea pigs and sheep pox diseases in sheep after challenge with virulent Bacillus anthracis and sheep pox virus respectively. The vaccine was found stable after 15 month of preservation at -20C.
  Haroun, M. , I.E. Hajer , M.M. Mukhtar and T. Barrett
  Humoral and Cell-Mediated Immune (CMI) responses of four Angus calves following vaccination and challenge with rinderpest viruses were investigated. Both of two calves vaccinated with the rinderpest bovine ‘O` Kabete strain (RBOK) seroconverted with a mean peak percentage inhibition (PI) of 80.5 56 days post vaccination. Mean peak PI of 82 was demonstrated by sera of the vaccinated calves when challenged with the virulent rinderpest virus (RPV)-Saudi 1/81 strain 63 days post vaccination. Lymphoproliferative responses (LPR) of the peripheral blood mononuclear cells (PBMC) measured by cellular DNA {3H}thymidine uptake revealed mean peak stimulation index (SI) of 10 at day 35 of vaccination. Mean peak SI of 13.2 was attained 2 days post challenge. PBMC of the infected control calves did not respond to stimulation. The two vaccinated calves did not succumb to challenge. Based on the pattern of the responses and reaction to challenge, protection against infection seemed to be regulated by the cell-mediated immune system rather than the humoral pathway alone.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nour and M.A. Fadol
  The combined anthrax and sheep pox vaccine was prepared in a lyophilized form containing the recommended doses of both sheep and goat strain 0240 pox and anthrax live spore vaccine. Immunization of sheep with this vaccine revealed a considerable immune response when detected with immunocapture Enzyme Linked Immunosorbent Assay (ELISA), indirect ELISA and with (3-(4, 5-dimethylthiazolyl-2) -2, 5-diphenyltetrazolium bromide) (MTT) lymphocytes proliferation assay. The difference in titers of antibodies raised against capripox virus antigen was significant, it was also significant for antibodies raised against crude Protective Antigen (cPA) and killed spore antigen. Phytohemagglutinin (PHA) revealed high Stimulation Index (SI) before vaccination but low one after vaccination while the SPPV antigen showed slight SI before vaccination and high SI after vaccination indicating cellular immune response to this antigen. Low SI of PHA after vaccination might be due antimitogenic effect of SPV antigens.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ElHussein , Tageldin A.M. Nour and M.A. Fadol
  The immune response of Sudanese sheep vaccinated with capripox vaccine was detected by IFA, Ic-ELISA test and MTT lymphocytes proliferation assays. The difference between antibodies titers before and after vaccination was found significant when tested by IFA test and the difference between the mean OD values was also significant when Ic-ELISA was used. PHA was found more effective in stimulating peripheral blood lymphocytes before vaccination compared with the virus antigen. After vaccination, the mean stimulation index of the of the virus antigen was higher
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