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Articles by M.M. Amin
Total Records ( 3 ) for M.M. Amin
  M.B. Rahman , M.M. Rahman , M. Rahman , S.M.L. Kabir , K.H.M.N.H. Nazir and M.M. Amin
  Thirty day-old chicks of Cobb-100 breed with the history of vaccination of parent stock against Newcastle disease (ND) were equally divided into two groups such as group A and B. At 7 days of age of birds, group A was vaccinated with experimentally prepared V4HR-ND vaccine via eye drop @ 106.0EID50/bird to determine the efficacy of this vaccine while group B was kept as unvaccinated control. The birds were used for the study during the period from October 2003 to December 2003. Each bird of group A was boostered with same vaccine @ same dose at 24 days of age. Both the groups (A and B) were challenged after two weeks of post-booster vaccination with 0.25ml inoculum containing 105.0EID50 of virulent field isolates of ND virus intramuscularly. The results of challenge experiment revealed that six birds (40%) of group A succumbed within 3 to 4 days of post-challenge, whereas 15 (100%) unvaccinated control birds of group B showed clinical illness and ultimately died within 3 to 5 days. Thus, the experimental V4HR-ND vaccine conferred 60% protection of vaccinated birds against challenge infection. The mean values of Haemagglutination inhibition (HI) antibody titres of birds in group A were found to have significantly (P<0.01) increased at two weeks of post-booster vaccination. These results indicated that experimental V4HR-ND vaccine induced sufficient humoral immune response which gives satisfactory level of protection against ND.
  M.T. Hossain , M.A. Islam , M.M. Amin and M.A. Islam
  The comparative efficacy between the conventional vaccine (DLS-DPV) and experimentally prepared duck plague vaccine (BAU-DPV) was evaluated in seventy-five 35-day-old Zinding breed ducklings during the period from October/02 to March/03. The ducklings were equally divided into five groups (A, B, C, D and E). Ducklings of group A and B were primarily vaccinated with 0.5 ml and 1.0 ml of DLS-DPV respectively and those of group C and D were primarily vaccinated with 0.5 ml and 1.0 ml of BAU-DPV. The ducklings of group E were kept as unvaccinated control. Five months after primary vaccination all the ducks of vaccinated groups were boosted with 1.0 ml of DLS-DPV and BAU-DPV and 21 days after booster vaccination all the ducks of vaccinated and control groups were challenged with 1ml of 10 4 EID50 of virulent field isolate of duck plague virus (DPV). The level of immunity developed in different vaccinated groups of ducks was measured by passive hemagglutination (PHA) test. The mean PHA titre of birds of group A, B, C and D after primary vaccination were 38.4 ± 6.4, 28.8 ± 3.2, 51.2 ± 7.84 and 38.4 ± 6.4 and after booster vaccination were 153.6 ± 25.6, 76.8 ± 12.8, 358.4 ± 62.71 and 115.2 ± 12.8 respectively. Results of PHA test indicated that experimentally prepared duck plague (BAU-DPV) vaccine revealed higher immune response compared to that of the conventional (DLS-DPV) vaccine and results of the challenge experiment indicated that the mean PHA titre over 100 after booster vaccination revealed 100% protection.
  M.K. Hossain , M.M. Amin , M.S.R. Khan , M.R.R. Sarker , Y.Akhter and S.M.H. Rashid
  The aim of the study is to detect the causes of vaccination failure in chickens against IBD in Bangladesh. One selected local field IBDV isolate, one commercially available live attenuated IBDV vaccine Bur-706 (RP) and one killed IBDV vaccine Gumboriffa (RP) were used in this study. A total of 100 chicks (Star brow) of 0,7,14,21 and 28 days age group (taking 20 in each group) were used for study the serologic response with a selected local field IBDV isolate. Similarly two other vaccines were vaccinated in each 100 chicks of similar age groups. After collection of sera weekly for upto six weeks from all groups of birds they were subjected to SNT, PHA test and AGPT against a specific IBDV antigen. Through this investigation it is found that the local field IBDV isolate demonstrate higher serologic response than that of commercially available live or killed IBDV vaccines. So, it may be concluded that the commercially available imported vaccines can not induce sufficient immune response to protect the chickens in Bangladesh. It may be due to degradation of the vaccine quality during transportation or in a new environmental condition or due to antigenic dissimilarities among the local field virus and the imported vaccines.
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