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The aim of the study was to evaluate the protein similarity
degree among 15 C. albicans isolates with different virulence obtained
from healthy and infected human and animals. Yeast cells were grown in
YPG medium and collected by centrifugation. After cell wall disruption,
the cell wall and cytoplasmic proteins were submitted to PAGE and SDS-PAGE
techniques. Similarity degree was determined using the Dice similarity
coefficient (SD). Candida albicans isolates showed high
similarity together with average 86.2% (78.1<SD<100).
Regarding to the virulence and host aspects, the means SD of
high virulent and low virulent isolates as well as human and animal isolates
were 85.8 and 85.6% in PAGE, respectively. Considering SDS-PAGE, electrophoregrams
of C. albicans cytoplasmic proteins of low and high virulent isolates
as well as human and animal isolates showed the similarity about 90%.
The mean SD of cell wall was 91.9% between low virulent and
high virulent isolates and 92.2% between human and animal isolates. Such
results suggest a highly protein similarity degree among the most isolates
with low and high virulence as well as from human and animal hosts. The
whole-cell protein profile obtained by these techniques could provide
additional criteria for the serologic and immunologic studies of C.
albicans.
