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Articles by M.K. Singh
Total Records ( 3 ) for M.K. Singh
  Manoj Jinu , R.K. Agarwal , B. Sailo , M.A. Wani , Ashok Kumar , K. Dhama and M.K. Singh
  The aim of the study was to compare Polymerase Chain Reaction (PCR) and conventional method for detection of Salmonella from field poultry samples (n = 510, poultry blood and faeces 255 each). The prevalence rate of Salmonella in chicken was found to be 5.09% using conventional method and 5.88% by PCR assay. Serotyping of 26 Salmonella isolates revealed 57.69% Salmonella Typhimurium, 19.23% rough type, 15.38% Salmonella Enteritidis and 7.69% untypable. Among Salmonella Typhimurium isolates, 73.33% were from poultry blood and 26.66% from faeces samples. All isolates belonging to Typhimurium and Enteritidis serotypes were confirmed by PCR targeting of Salmonella Typhimurium (typh) and Salmonella Enteritidis (ent) specific genes. However, 4 isolates found to be rough type also turned out to be positive for ent gene. The PCR employed for detection of Salmonella was found 100% sensitive for poultry blood but its sensitivity was very less (77.77%) for faeces samples as compared with culture method. However, PCR was 100% specific with regard to faeces samples. The specificity from blood samples was 97.89% by PCR. The positive predictive values of PCR from blood and faecal samples were 77.27 and 100% with a concordance of 98.03 and 99.21%, respectively. The negative predictive values from blood and faecal samples were 100 and 99.19%. The study demonstrated usefulness of genus specific PCR for detection of Salmonella in poultry clinical samples. Owing to its robustness and rapidity it can be used for wide epidemiological studies. Serotype specific PCR detection of Typhimurium and Enteritidis serotypes has added advantage in identifying them even where there is loss of O antigen.
  R. Kumar , R.M. Reddy , P.S. Sinha , J. Tirkey , M.K. Singh and B.C. Prasad
  The continuous exploitation of tropical tasar food-plant, Terminalia tomentosa (W and A) rose on wastelands under the rain-fed conditions by rearing its economic insect pest; Antheraea mylitta (D) needs soil management for sustainable yields. To asses the feasibility, legumes like Sun hemp (Crotalaria juncea), Daincha (Sesbania aculeata), Green gram (Vigna radiatus) and Black gram (Vigna mungo) were raised during monsoon (June) season in the inter space of T. tomentosa economic plantation for biomulching. The Sun hemp has yielded highest biomass of 39.0 q ha -1, followed by Daincha, Green gram and Black gram with 26.6, 25.3 and 17.7 q ha -1, respectively after 45 to 50 days of sowing, has been mulched for soil fortification. The water holding capacity, electrical conductivity and organic carbon have been improved with reduced pore space and bulk density of the soil and enhanced leaf yield of tasar food-plant after legume biomulching. The superior Effective Rate of Rearing (ERR), higher cocoon and shell weights, better silk ratio and longer silk filament length of tasar silkworm in biomulch treatments have contributed to increase silk yield. Among the biomulches, Sun hemp has shown improved water holding capacity (56.9%) and moisture (68.5 and 37.9%) at 30 and 45 cm depth with electrical conductivity (0.30 mhos cm -1), bulk density (1.12 g cm -3), pore space (50.4%), organic carbon (0.61%) of soil, leaf yield (24.8 MT ha -1) of food plant and ERR (80.1%), cocoon weight (12.25 g), shell weight (1.90 g), silk ratio (14.03%) and silk filament length (724 m) specify its suitability in making tropical tasarculture sustainable under rain-fed conditions.
  M.K. Singh , J.P. Rocca , C. Rochon , M.E. Facciuto , P.A. Sheiner and M.I. Rodriguez-Davalos

Background: Abdominal wall closure after liver transplantation is not always feasible and may result in increased intra-abdominal pressure along with associated complications. Various temporary closure techniques as well as open wound management have been used to address this complex problem. The aim of this series was to describe an approach to definitive wound closure of the open abdomen in liver transplant patients.

Methods: We performed a retrospective review of all liver transplant patients at our institution from September 2005 to November 2007. The management of the open abdomen in 10 liver transplant patients was reviewed, and a novel approach described to manage these defects.

Results: Ten patients with open wounds were closed during the study period using human acellular dermal matrix (HADM). There were 7 men and 3 women of median age 55 years. Average size of HADM was 235 cm2. The median follow-up is 10 months with no incidence of evisceration or hernia. In 1 patient, the graft failed along the lateral side due to infection; it dislodged during vacuum-assisted closure dressing change in another patient at 5 months after closure. Fascial closure was not possible due to organ edema (n = 3), a large liver (n = 4) or wound infection with dehiscence (n = 3).

Conclusions: HADM can be used for primary wound closure in both clean and contaminated wounds as an alternative to an open abdomen post-liver transplantation.
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