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Articles by M.H. Motazedian
Total Records ( 5 ) for M.H. Motazedian
  Q. Asgari , D. Mehrabani , M. Moazzeni , F. Akrami-Mohajeri , M. Kalantari , M.H. Motazedian and G.R. Hatam
  This study determines the prevalence of ovine toxoplasmosis in Shiraz, Southern Iran. From April 2004 to May 2005, serum samples of 603 sheep were randomly collected in 18 Cities of Fars Province, Southern Iran and tested for toxoplasmosis using Indirect Fluorescent Antibody Technique (IFAT). The prevalence of toxoplasmosis was 26.5%, while the rate of seropositivities in 1/100, 1/200, 1/400 and 1/800 dilutions were 17.7, 2.8, 4.3 and 1.7%, respectively. The highest prevalence was in Abadeh (56.7%) and Nourabad (44.3%) cities and the lowest was determined Arsanjan (4.2%) whereas no infection was determined in Fasa. Considering the high prevalence of toxoplasmosis in sheep in our region, control measures need to be undertaken to prevent transmission of the infection to other animals and man by health and veterinary authorities. Therefore, it seems that standardization of techniques, hygienic standards in sheep breeding especially in cities with more migrating domestic animals and environmental health education for veterinary personnel are required to prevent human infection.
  Q. Asgari , D. Mehrabani , M. Moazeni , F. Akrami Mohajeri , M. Kalantari , M.H. Motazedian , G.R. Hatam and M.A. Davarpanah
  This study evaluates the seroprevalence of bovine toxoplasmosis in Fars province, Southern Iran. From March 2004 to April 2005, 588 bovine serum samples were randomly provided from 15 cattle slaughterhouse of 15 cities and towns in Fars province, Southern Iran that were divided into 5 districts including the central, Northern, Southern, Eastern and Western parts. Indirect Fluorescent Antibody Test (IFAT) was used to determine the antibody titer. The seroprevalence of toxoplasmosis was 20.24% (119 cattle) while the rate of seropositiveness in 1/16, 1/32, 1/64 and 1/128 dilutions were 15.31% (90), 3.74% (22), 0.85% (5) and 0.34% (2), respectively. The highest prevalence was in Eastern (23.33%) and Central parts (22.73%) and the lowest (2.33%) was noticed in Northern region of the province. The highest prevalence (37.5%) was observed in Mamasani. However, the lowest prevalence (zero) was found in Abadeh, Marvdasht, Arsanjan and Firouzabad cities. So, control measures such as environmental health education of veterinary personnel and standardization of techniques and hygiene in bovine breeding seem necessary to prevent transmission of the infection to human.
  D. Mehrabani , M.H. Motazedian , G.R. Hatam , Q. Asgari , S.M. Owji and A. Oryan
  This study was undertaken to identify rodents infected with Leishmania in Fasa town, Fars Province, Southern Iran. From April 2004 to April 2005, a total of 17 rodents were collected in live traps in the area. Microscopical examination of stained tissue smears and the culture of tissue samples, PCR, isoenzyme electrophoresis and morphological review were used for detection of the parasite. The captured rodents were 10 Rathus rathus and 7 Tatera indica. One male T. indica was infected with L. major in Zahedshahr District of Fasa town. In histological and ultrastructural studies, numerous clusters of amastigotes were seen in the foamy macrophages of bone marrow of the femoral bone. We can conclude that T. indica is one of the rodents that may be a potential reservoir host of L. major in the region and femoral bone marrow is morphologically the tissue of choice to confirm the presence of macrophages containing the amastigote form of the parasite.
  Q. Asgari , D. Mehrabani , M.H. Motazedian , M. Kalantari , J. Nouroozi and S.J. Adnani Sadati
  This study was undertaken to evaluate the viability and infectivity of the tachyzoites in different temperatures and times of milk by bioassay methods. Tachyzoites of RH strain were transferred into sterile milk and kept in different temperatures (4, 20 and 37°C) for different time intervals (10 and 30 min) and their viability was evaluated by vital stain (methylene blue) and the infectivity by intra-peritoneal and oral inoculation to different groups of inbred BALB/C mice (2-4 mice in each group obtained from Pasteur Institute, Tehran, Iran). Tachyzoites in saline were intra-peritoneally inoculated to inbred BALB/C mice as positive controls. All animals were kept in Laboratory Animal Center of Shiraz University of Medical Sciences in Shiraz, southern Iran. The touch smear of spleen and liver of dead mice were stained by Giemsa and were parasitologically evaluated. Eighty percent of tachyzoites were stained by methylene blue indicating identical parasite viability. All mice died after intra-preritoneal inoculation of tachyzoites present in sterile milk. Only one out of eight mice which was orally inoculated remained alive. All mice fed by tachyzoites in saline died. Duration of mice vitality was more in oral route than peritoneal inoculation (n1 = 11, Mean1 = 9.55 days and n2 = 17, Mean2 = 6.65 days, p = 0.001). Tachyzoites in drinks and foods undergoing heating up to 37°C for 30 min such as milk can be a possible route of transmission of toxoplasmosis to human. Therefore strict public health measures should be carried out to prevent transmission of disease in high risk areas.
  Q. Asgari , F. Akrami Mohajeri , M. Kalantari , B. Esmaeilzadeh , A. Farzaneh , M. Moazeni , S.R. Ghalebi , F. Saremi , M. Zarifi Kalyani and M.H. Motazedian
  Since the meat of chicken is considered one of the sources of the human infection, this study was undertaken to compare the prevalence of toxoplasmosis in free-ranging with semi-industrial and industrial chickens (Gallus gallus domesticus) in Shiraz, Southern, Iran. 203 serum samples from free-ranging chickens of sub-urban districts, 50 serum samples of semi-industrial chickens which rearing in restricted location and 444 serum samples of industrial chickens from slaughters were collected and tested for toxoplasmosis by IFAT. Overall the rate of Toxoplasma infection in chicken was 10.04%. The prevalence of toxoplasmosis among free-ranging chickens was 27.1% but the rate in semi-industrial and industrial chickens was 12% and 2.02% respectively. The rate of seropositive chickens in titers of 1/16, 1/32, 1/64, 1/128, 1/256 and 1/512 was 5.88 %, 2.44 %, 1 %, 0.57 %, 0 %and 0.14 % respectively. Considering the high prevalence of toxoplasmosis in chickens, in the regions under study, control measures should be taken to prevent transmission of the infection to the animals and humans by Health and Veterinary organizations.
 
 
 
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