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Articles by M.H. Bejo
Total Records ( 5 ) for M.H. Bejo
  T.V. Thu , Loh Teck Chwen , H.L. Foo , Y. Halimatun and M.H. Bejo
  Problem statement: Hypercholesterol and fatty acids in plasma are the main causes for cardiovascular disease. Reduction of risk factors from diet that associated with cardiovascular disease has much attention in animals as well as in human. The objective of this study was to investigate the effects of feeding liquid metabolite combinations produced by five L. plantarum strains on the fatty acids and cholesterol concentration in plasma of postweaning piglets. Approach: A total of 120 postweaning piglets aged 26 day olds (Large White x Landrace x Duroc) were randomly assigned into one of five treatments. (i) basal diet with free antibiotic (-ve control); (ii) basal diet with 0.03% of chlortetracycline antibiotic (+ve control); (iii) basal diet with 0.3% metabolite of TL1, RG11 and RI11 (Com 1); (iv) basal diet with 0.3% metabolite of TL1, RG14 and RS5 (Com 2); (v) basal diet with 0.3% metabolite of RG11, RG14 and RI11 (Com 3). The experiment was conducted for 5 weeks. Fatty acids were analysed by Gas Chromatography (GC) and cholesterol was detected using commercial diagnostic kit. Results: The piglets fed with metabolite combinations were found to reduce plasma cholesterol and Saturated Fatty Acids (SFA) concentrations, particularly in Com 2 group which was significantly lower (p<0.05) than the -ve control group. In contrast, the Unsaturated Fatty Acids (USFA) were significantly higher (p<0.05) in Com 2 than -ve control group. The ratio of USFA and SFA was significantly higher (1.14%) in Com 2 as compared to -ve control group. However, there was significantly lower (p<0.05) in Com 3 as compared to control groups for the ratio of omega-6 and omega-3 in plasma of piglets. Conclusion: Metabolite combinations produced by L. plantarum strains have potential effects in influencing the lipid contents and reducing the cholesterol profile of the pig’s plasma.
  E. Maroufyan , A. Kasim , S.R. Hashemi , T.C. Loh and M.H. Bejo
  The aim of this study was to find the effect of methionine and threonine supplementations higher than the NRC recommendation on growth performance and white blood cell differentiation of broiler chickens challenged with infectious bursal disease. A total of 450 day-old male broiler chicks were assigned to nine groups. Chickens were fed by three graded levels of DL- methionine [NRC (M1), 2 times NRC (M2) and 3 times NRC (M3)] and three graded levels of L-threonine [NRC (T1), 2 times NRC (T2) and 3 times NRC (T3)] from day 1-42 of age. On day 28, all birds were challenged with a commercial live-IBDV vaccine. Body Weight Gain (BWG) and Feed Intake (FI) and Feed Conversion Ratio (FCR) were significantly influenced by the dietary treatments before challenge and either methionine or threonine at the highest levels significantly decreased BWG, FI and FCR in broiler chickens. Birds were fed with M3T3 had the lowest body weight gain after challenge. Supplementation of two times methionine and threonine (M2T2) to the diet decreased peripheral blood heterophils and increased lymphocytes and H/L ratio on day 28. On day 42, complete white blood cell tended to increase with increasing level of methionine supplementation. Threonine did not affect peripheral blood differential leukocyte count of broiler chickens. In conclusion, our data suggest that the methionine and threonine requirement of male broiler chicks is higher for growth performance than was suggested by the last NRC committee and methionine and threonine higher than NRC requirements in tropical condition can ameliorate the negative effects of heat stress.
  M. Emadi , K. Kaveh , M.H. Bejo , A. Ideris , F. Jahanshiri , M. Ivan and R.A. Alimon
  An experiment was conducted to determine effects of dietary Arginine (ARG) on growth performance and blood serum parameters in broiler chickens. A corn-soybean meal based diet containing different levels of ARG (0, 0.67, 1.37, 2.07 and 2.77) for the starter (0, 0.53, 1.1, 1.68 and 2.25) for the grower and (0, 0.52, 1.04, 1.56 and 2.08) for the finisher was used. In a completely randomized design with five treatments of five replicates each and 10 chickens per replicate, 250 Cobb 500 male broiler chickens from 0-49 days of age were used. Growth performance (body weight gain, feed intake and feed: gain ratio) and blood serum (albumin, total protein, glucose, cholesterol, triglyceride, urea, uric acid, aspartate amino-transferase, alanine amino-transferase, alkalin phosphatase, lactic dehydrogenase and creatine kinase) parameters were measured at 27 and 49 days of age. Increase of dietary ARG increased (p<0.05) body weight gain, feed intake, albumin, creatine kinase, glucose, urea and uric acid and decreased (p>0.05) aspartate amino-transferase and cholesterol. It was concluded that dietary ARG might have positive effects on health status of the broiler chickens.
  M. Emadi , F. Jahanshiri , F. Azizi Jalalian , K. Kaveh , M.H. Bejo , A. Ideris , A.A. Assumaidaee and R.A. Alimon
  Infectious Bursal Disease (IBD) continues to pose potential threat to poultry industry all over the world. The disease can spell disaster not only through its infection but also by break of immunity in chickens vaccinated for other diseases. On the other hand, arginine (Arg), a ubiquitous, semi-essential amino acid has emerged as an imunostimulant from variety of human and animal studies. In the present study, we demonstrate the stimulatory effects of Arg on systemic immune response in chickens challenged by orally administration of intermediate plus strain of IBD virus at 28 days of age. A corn-soybean meal based diet containing different levels of Arg (0, 0.67, 1.37, 2.07 and 2.77) for the starter, (0, 0.53, 1.1, 1.68 and 2.25) for the grower and (0, 0.52, 1.04, 1.56 and 2.08) for the finisher was used. In a completely randomized design with five treatments of five replicates each and 10 chickens per replicate, 250 Cobb500 male broiler chickens from 0-49 days of age were used. To measure the innate, cellular and humoral immunity indicators (interferon-α, interferon-γ, immunoglobulin G) at 27, 35, 42 and 49 days of age, serum samples from each replicate of treatments were collected and subjected to ELISA. The result showed that Arg supplementation in the chickens basal diets significantly increased the serum levels of interferon-α, interferon-γ, immunoglobulin G at 35, 42 and 49 days of age (p<0.05). The different levels of Arg at 27 days of age did not significantly affect interferon-α, interferon-γ, whereas Arg at 27 days of age significantly increased immunoglobulin G (p<0.05). These results revealed that arginine stimulates systematic immune response against intermediate plus strain of IBDV.
  M.R. Rosyidah , T.C. Loh , H.L. Foo , X.F. Cheng and M.H. Bejo
  A study was conducted to study the effects of feeding metabolites which produced from L. plantarum and acidifier in the diets of broilers chickens on growth performance, microflora count, digesta and faecal pH, immunoglobulin status and volatile fatty acids. A total of 288 male Cobb randomly assigned to five dietary groups for 42 days, basal diet feed (negative control), basal diet feed+neomycin and oxytetracyline (positive control), basal diet feed+0.1% acidifiers (A), basal diet feed+0.5% metabolite (M), basal diet feed+0.1% acidifiers (A) and 0.5% metabolite (M). Higher final body weight and weight gain, lower daily feed intake and feed conversion ratio were found in metabolites and combination of metabolite and acidifier groups while greater lactic acid bacteria count, low faecal and digesta pH and increase volatile fatty acids were found in 3 treated groups. No significant difference was found for immunoglobulin level.
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