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Articles by M.F. Alam
Total Records ( 3 ) for M.F. Alam
  M. Anisuzzaman , S. Jarin , K. Naher , M.M. Akhtar , M.J. Alam , M. Khalekuzzaman , I. Alam and M.F. Alam
  A viable protocol has been developed for indirect shoot organogenesis of okra. To establish a stable and high-frequency plant regeneration system, leaf disc and hypocotyl explants were tested with different combinations of α-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA),iIndole-3-butyric acid (IBA), thidiazuron (TDZ) and 6-benzylaminopurine (BAP). Morphogenic callus induction was observed in highest frequency from hypocotyl explant by culturing in MS medium supplemented with 2.0 mg L-1 NAA plus 0.5 mg L-1 TDZ. The highest percentage of shoot regeneration and highest mean number of shoot per callus mass was obtained with 2.0 mg L-1 BAP plus 0.1 mg L-1 IBA. Root formation was observed from callus induced in medium containing 1.5 mg L-1 NAA. Morphogenic difference due to explant type is clear for the studied in vitro traits. About 80% of regenerated plantlets were survived and showed new leaves development under ex vitro condition. This protocol would be useful to create somaclonal variation and to utilize transgenic approach for varietal improvement of okra.
  A.M. Shohael , M.A.L. Akanda , S. Parvez , S. Mahfuja , M.F. Alam , R. Islam and N. Joarder
  The present study was carried out to develop protocol in which plants could be regenerated with easy and in high numbers from tissue cultures of maize. The tissues of immature embryos were tested in three inbred lines (CML-161, CML-323, CML-327) MS and N6 were used. In N6 medium, the range of embryogenic calli formation was varied from 56.33-72.0%. The results indicated that the highest amount 72.0% was obtained when N6 was supplemented with L-proline 2.3 gm l-1, Casein Hydrolysate 200 mg l-1 and 2,4-D 1.0 mg l-1. Where as in MS medium the embryogenic calli formation was varied from 39.0-68.66%. In this case the highest amount (68.66) was obtained where MS was supplemented with L-Asparagine 150 mg l 1, Thiamin 50 mg l-1 and 2,4-D 1 mg l-1. Plants were regenerated successfully from embryogenic callus in hormone free MS medium. The results showed that MS (52.83-61.0%) medium was found better than N6 (35.66-42.49%). The mode of somatic embryogenesis was studied using histological technique.
  M. Anisuzzaman , S.A. Sharmin , S.C. Mondal , R. Sultana , M. Khalekuzzaman , I. Alam and M.F. Alam
  In vitro microrhizome was successfully produced in Curcuma zedoaria-a valuable but critically threatened medicinal plant. Ten to twelve weeks old in vitro multiplied shoots were cultured in MS medium supplemented with different level of BAP and NAA. Effect of different carbon sources has also been evaluated. Microrhizome formation started within 7-9 weeks of culture. The best result was obtained in 4.0 mg L-1 BAP along with 6% sucrose in terms of frequency and number of rhizome. Around 4 microrhizomes with an average weight of 2.5 g were harvested after 11-12 weeks. Microrhizomes were readily germinated in the growth regulator free MS medium. With the best of our knowledge this is the first report on in vitro microrhizome production in Curcuma zedoaria.
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