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Articles by M.A. Ibrahim
Total Records ( 15 ) for M.A. Ibrahim
  Kh.A. El-Dougdoug , A.A. Megahed , B.A. Othman , S.M. Lashin , M.A. Ibrahim and Idress Hamad Attitalla
  Systemic Acquired Resistance (SAR) could be induced in cucumber plants using different individual of seven microbial isolates against Cucumber mosaic Cucumovirus (CMV). These isolates were Bacillus subtilis, Bacillus polymyxa, Bacillus circulans, Pseudomonas putida, Pseudomonas fluorescens 2 and Pseudomonas fluorescens 8 as bacterial isolates and Trichoderma harzianum as fungal isolate. The occurrence of SAR was found by seed treatment with microbial liquid culture based on virus infectivity and the level of free and endogenous salicylic acid (SA), 14 days from CMV inoculation. Seven biotic inducers reduced CMV infectivity at range 16.6 to 39% and T. harzianum gave the highest percentage of reduction 39%. In addition, the level of total SA has been increased in treated plants, B. subtilis gave the highest level of SA 239.13 μg g-1 fresh weight (fwt) while, B. circulans gave the lowest level 70.1 μg g-1 fwt.
  M.A. Ibrahim , N.D. Al-Hmoud , H. Al-Rousan and B.O. Hayek
  Taking into account the impact of monitoring food adulteration on the quality of food products, the aim of this study was to use polymerase chain reaction technology to detect possible adulteration of durum wheat pasta products in the Jordanian market. Cetyltrimethyl ammonium bromide method was applied for extracting genomic DNA from twenty six randomly collected pasta products, the results suggested the suitability of this method for DNA extraction from pasta products. Specific primers were used to ascertain the presence or absence of Dgas44 sequence in the extracted genomic DNA from pasta products, this sequence is known to be present in the genome of common bread wheat and absent in the genome of durum wheat. The obtained results showed that 65.4% of tested pasta products sold in the Jordanian markets are not made solely from durum wheat as indicated by manufacturers.
  M.A. Ibrahim , K.A. Gihan , S.M. Aboelhadid and M.M. Abdel-Rahim
  Objective: The objective of this study was to determine the prevalence of zoonotic intestinal parasites in pet dogs and their owners in Cairo and Giza Governorates, Egypt. Methodology: A total of 395 fecal samples collected from pet dogs beside 145 stool samples from humans were subjected to macroscopic and microscopic examination using different flotation and sedimentation techniques. Results: The overall prevalence of enteric parasites in the examined dogs was 25.6%. Higher prevalence was shown in police dogs (43.3%) followed by pet shop dogs (30.8%) and finally in household dogs (13.8%). Cryptosporidium was the most frequent parasite detected in the examined dogs (10.1%) and humans (10.3%), whereas Giardia was the lowest one (0.5 and 2.8% respectively). In addition, Entamoeba histolytica/Entamoeba dispar were found at a rate of 5.6% (dogs) and 7.6% (humans). Trichuris vulpis, Toxascaris leonina and Toxocara canis were identified only from the examined dogs at a rate of 3.3, 5.8 and 0.3% respectively. Young age (<6 months), female sex, local breed, undercooked feeding, outdoor housing and irregular de-worming were significantly associated with increased prevalence of the identified parasites in dogs. Regarding humans, the highest prevalence of enteric parasites was found in the age group between 5-10 years old (60%). Gender did not affect the risk of an infection in the study population. Conclusion: In conclusion, parasitological results in this study clearly highlight the significant role of pet dogs as a host for several species of enteric parasites; therefore preventive measures should be taken to avoid the environmental contamination and infection of both man and animals.
  M.A. Ibrahim , A.B. Sallau , A. Salihu and K.C. Onwube
  Phospholipase A2 (EC. was isolated and partially characterized from the erythrocytic stage of Plasmodium berghei (NK-65) obtained from experimentally infected mice with the objective of studying its kinetic properties and its possible role in the pathogenesis of malaria. The parasite collected by sucrose gradient centrifugation was subjected to lysis to obtain the crude phospholipase A2 which was assayed and subjected to some biochemical characterizations . The enzyme had broad pH and temperature ranges with optima of 7.5 and 37°C, respectively. Initial velocity studies for the determination of kinetic parameters with L-α lecithin as substrate revealed a KM and VMAX of 0.68 mg mL-1 and 52.60 μmol min-1, respectively. The P. berghei PLA2 was slightly activated by Ca2+ while Cu2+, Zn2+and Mn2+ were inhibitory to the enzyme. However, no enzyme activity was detected in the presence of Mg2+ and Hg2+. Considering the findings of this work, P. berghei can be said to contain PLA2 which has similar properties with some other parasites PLA2 and could be involved in cellular invasion and/or aneamia development during cerebral malaria.
  N. Saleh , M.A. Ibrahim , E. Archoukieh , A. Makkiya , M. Al-Obaidi and H. Alobydi
  The aim of this study is to ascertain the possible application of Random Amplification of Polymorphic DNA (RAPD) analysis as a genetic test to investigate DNA polymorphisms and detection of genomic markers in various types of leukemia. The results showed unique profiles of amplified DNA fragments produced in genomic DNA of three types of leukemia by an arbitrary primer of decamer oligonucleotides OPA-09. The primer produced four types of amplified DNA fragments (980, 1659, 2187 and 3162 bp). The smallest amplified DNA fragment (980 bp) appeared in 14.3 and 13.3% of tested acute myeloid leukemia and chronic myeloid leukemia patients, respectively; but was absent in genomic DNA of chronic lymphoid leukemia and normal individuals. Whereas the largest amplified fragment (3162 bp) was present in 12.5, 20 and 75% of chronic lymphoid leukemia, chronic myeloid leukemia and normal individuals, respectively and was absent in acute myeloid leukemia. On the other hand, the two amplified fragments (1659 and 2187 bp) were present in normal and leukemia patients. Cluster analysis of amplified DNA fragments grouped the leukemia patients in two main groups. The detected DNA polymorphisms by the arbitrary primer OPA-09 might find application in developing efficient RAPD primer for diagnosis of leukemia.
  N. Al-Hmoud , H. Al-Rousan , B.O. Hayek and M.A. Ibrahim
  The objective of this study was to survey for the genetically modified maize and soybean food products in the Jordanian market. The study was designed to extract genomic DNA of maize and soybean products by cetyltrimethylammonium bromide (CTAB) method and to identify specific genes for maize and soybean, expression control specific genes 35S promoter and NOS terminator by polymerase chain reaction analysis. For confirmation test of the genetically modified maize and soybean food products, nested polymerase chain reaction experiments were performed using internal primers for the detection of the E35S promoter and the hsp70 exon1/intron1 region of maize MON810 and Cp4 EPSPS gene of soybean. Three out of 19 maize food products were identified as carrying amplified DNA fragments of 35S promoter region, the nested PCR test confirmed the presence of MON810 event. One of three soybean food products was identified as carrier DNA fragments of 35S promoter region, Cp4 EPSPS event was not detected.
  A. Makkiya , M.A. Ibrahim , N. Al-Hmoud , H.H. Hasani and H.M. Said
  Acute Myeloid Leukemia (AML) is the most frequent cause of acute leukemia affecting adults, its incidence increases steadily with age and has been correlated with DNA methylation aberration and inactivation of tumor suppressor genes. Recent epigenomic studies showed that DNA methylation abnormalities have been observed in age related acute myeloid leukemia and indicated the importance of DNA methylation analysis for AML diagnosis. An insight of the connection between DNA methylation and AML might help in development of novel molecular diagnostic and genomic therapies. Epigenetic drugs of two families namely the DNA-demethylating agents and inhibitors of histone deacetylase have emerged as the most promising compounds in this area and several pharmaceutical compounds have received approval for the treatment of specific leukaemia and lymphoma subtypes. In addition possible combination between molecular therapeutic approaches including the activation of certain signal transduction pathway(s) like the interleukins family and chromatin-remodeling events is feasible by the application of epigenetic drugs. This approach might be one of the potential promising solutions for the AML treatment. The present study reviewed recent advances in the research related to genomic and epigenomic of acute myeloid leukemia.
  A.B. Aliyu , M.A. Ibrahim , A.M. Musa , T. Bulus and A.O. Oyewale
  Leaf extract and fractions of Vernonia blumeoides were evaluated for total phenolics content (Folin-Ciocalteu method), free radical scavenging activity (1, 1-diphenyl-2-picrylhydrazyl radical assay), total antioxidant capacity (Phosphomolybdate assay) and ferric reducing power. The results of the phenolics content expressed in mg/100 g of Gallic Acid Equivalent (GAE) showed that the n-butanol fraction has significantly (p<0.05) higher phenolics content (410±0.8) than the chloroform fraction and ethanol extract. The radical scavenging activity of the extract and solvent fractions displayed strong concentration dependent activity. But it was also observed that the ethyl acetate fraction showed highest activity in all concentration tested ranging from 70.56 to 99.04%. However, the total antioxidant capacity (mg g-1 ascorbic acid) showed that n-butanol fraction has the highest capacity (60.0 mg g-1). The results also showed that the n-butanol extract has strongest reducing ability (2.105±0.109 nm) at higher concentration which is comparable to that of Gallic acid at all the concentrations tested. Phytochemical screening on the extracts revealed the presence of flavonoids, saponins and tannins. The results suggest that the plant especially the n-butanol and ethyl acetate fractions are very rich in antioxidant compounds worthy of further investigations.
  A.A. Khalaf , M.A. Ibrahim , A.F. Tohamy , A.A. Abd Allah and Amr R. Zaki
  Background: Chlorsan is highly effective bactericide and fungicide, so it is being widely used as insecticides in Egypt and the potential toxicity was raised more attention as it caused an oxidative stress and genotoxic effect in testicular tissue. Vitazinc, one of the most effective antioxidant and may play a role on preventing the toxic effect. Materials and Methods: Fourty mature Albino rats were divided into four groups (10 rats each). Group (1) control rats received orally an equivalent volume of corn oil on body weight. Group (2) rats in this group were orally administrated 1/10 LD50 of chlorsan equal to 1 mg kg–1 b.wt., dissolved in corn oil for 60 days. Group (3) male rat in the groups were orally administrated 1/10 LD50 of chlorsan in dose level of 1 mg kg–1 b.wt., beside 200 mg kg–1 b.wt., vitazinc. Group (4) rats in the group received orally 200 mg kg–1 b.wt., vitazinc only. Results: Chlorsan caused significant increase in lipid perioxidation. While significant inhibition in the activity of catalase (CAT), ChE activity and decrease in the level of reduced glutathione (GSH) were evident. Genotoxicity results revealed significant increase in the amount of m RNA of COX-2 and significant increase in the values of DNA fragmentation. Interestingly, pretreatment with Vitazinc attenuated these adverse effects. Vitazinc, therefore is a potent antioxidant and can protect against chlorsan-induced oxidative damage and genotoxicity by reducing lipid peroxidation and enhancing the antioxidant defense mechanisms. Histopathological examination revealed marked changes in testes of male treated rats. Conclusion: The present study reveals that vitazinc is effective in attenuating the oxidative stress, genotoxicity inflicted by chlorsan toxicit.
  A.A. Megahed , Kh.A. El- Dougdoug , B.A. Othman , S.M. Lashin , M.D. Hassanin , M.A. Ibrahim and A.R. Sofy
  Cucumber mosaic cucumovirus beet Egyptian isolate (CMV-Beet-EG) was detected serologically by DAS-ELISA using polyclonal antibodies in naturally infected sugar beet plants. The virus was isolated by single local lesion method on Chenopodium amaranticolor and then, propagated on sugar beet plants. The virus isolate was confirmed serologically by using dot and tissue printing immunoassay. The coat protein gene of CMV-Beet-EG was successfully amplified using specific primer by RT-PCR and the expected size was 820 bp. Sequence analysis cp gene revealed 97.3 and 96.4% identified similarity with CMV strains sequences published in GeneBank. The highest content of nitrogen bases was for Thymine (T) 164 (26.5%) followed by Cytosine (C) 163 (26.3%), then Adenine (A) 148 (23.9%) and Guanine (G) 144 (23.3%). The ratio of A/T and G/C were 0.902 and 0.883, the percentage of A+T and G+C was found 50.4 and 49.6. The deduced amino acids sequence of CMV-Beet-EG/CP gene was 206 amino acids with a molecular weight of 22.983 kDa and isolelectric point of 9.95. There are total 20 amino acids, the amino acid Serine (S) has the highest content and ferquency of all amino acids 25 and 12.1% while the lowest content and frequency of amino acid was 1 and 0.5% to the each of amino acid Histidine (H) and Trpyptophan (W). The CMV-Beet-EG/CP gene sequence was recorded in GeneBank with Accession No. JX826591.
  A.A. Megahed , Kh. A. El- Dougdoug , B.A. Othman , S.M. Lashin , M.A. Ibrahim and A.R. Sofy
  Cucumber mosaic cucumovirus satellite strain Egyption isolate (st-CMV-EG) was detected using polyclonal antibodies by DAS-ELISA form naturally infected cucumber leaves collected from protected agriculture. The samples which give positive reaction were used to virus isolation. st-CMV-EG isolate has a wide host-range belonging to 6 families. The virus isolate was inactivated at 70°C, it was infected at dilution 10-4 and longevity was 4 days at room temperature. Amorphous and crystalline inclusion bodies were detected in hair cells of epidermal strips of CMV-inoculated cucumber leaves. The U.V absorption ratio, A260/A280 and Amax./Amin. were 1.204 and 1.101, respectively, as well as virus yield was 1.43 mg/100 g fresh leaves in purified virus preparation. Electron micrograph of the purified virus isolate showed spherical particles (28 nm.). The virus isolate was detected serologically by using dot blot immunoassay. CMV-RNA was successfully detected in infected cucumber leaves using specific primer of cp gene by RT-PCR and the expected size was about 582 bp. Sequence analysis of CMV-cp gene of cucumber Egyption isolate was indicated 38% similarity to that of AB024493 and D00542.
  M.I. EL-Gohary , A.S.A. Mohamed , M.M. Dahab , M.A. Ibrahim , A.A. El-Saeid and H.A. Ayoub
  A common feature of epilepsy in EEG signals is an excessive electrical discharge which is appeared as electrical potentials of high amplitudes and frequencies with abrupt onset and rise in amplitude, rythmicity and abnormal synchronization. These potential discharges were termed Seizure patterns. Although several details concerning the cellular basis of these seizure patterns are unknown, numerous experiments led to the general agreement that they reflect a spontaneous and uncontrolled firing of a large number of neurons within a certain region of the brain. Artificial Neural Network (ANN) was proposed in this research as a decision-making tool supported by experimental data to differentiate between healthy and epileptic EEG signals, with accuracy up to 90.2%. This was done by teaching the ANN to perform this function i.e., by Artificial Intelligence (AI) of ANN. The performance of the ANN was calculated for each model`s node to obtain the performance of the node. ANN approach is a powerful tool which is promising to give available results in analysis of bioelectric signals.
  A.B. Isah , Y.K.E. Ibrahim , E.M. Abdulrahman and M.A. Ibrahim
  The hypoglycaemic activity of the aqueous extract of Stachytarpheta angustifolia (Verbanaceae) was studied in normoglycaemic and alloxan-induced diabetic rats. The extract was administered orally to the rats and blood glucose level was monitored for 7 h. Results indicate that the aqueous extract (750 mg kg-1) produced a significant blood glucose reduction in both normoglycaemic and alloxan-induced diabetic rats (p<0.05). The present result therefore appears to support the use of the plant aqueous extract for the management of type 2 diabetes by traditional medical practitioners in Northern Nigeria. The mechanism of action of the aqueous extract needs to be studied.
  A.A. Megahed , Kh. A. El-Dougdoug , B.A. Othman , S.M. Lashin , M.A. Ibrahim and A.R. Sofy
  The possibility of making use of the phenome non of Systemic Acquired Resistance (SAR) to control viruses achieved by the soaking treatment of tomato seeds cv. Castl Rock with three growth forms to Bacillus circulans, Pseudomonas fluorescens 2 and Trichoderma harzianum against Tomato mosaic tobamovirus (ToMV) infection. All the application forms of beneficial biotic inducers were reduced the mean number of ToMV local lesions on Datura metel. P. fluorescens 2 was found to be the best treatment in three forms on reduction of local lesion number 42.2, 32.7 and 38.1 of microbial liquid culture, microbial cells or spores and microbial culture filtrate forms, respectively, while the highest mean numbers of local lesions were 51.5, 61.7 and 73.5 of microbial liquid culture, m icrobial cells or spores and microbial culture filtrate, respectively for T. harzianum. The microbial culture filtrate form was more effective than other microbial forms to reduce mean number of ToMV local lesions to B. circulans, P. fluorescens 2 and T. harzianum isolates, 40.7, 32.1 and 51.5, respectively. The individual microbial isolates on all three microbial forms able to vary ToMV local lesions similarity (homologous or heterologous) and morphology (size center and surrounded with halo or without halo) compared with TMV mother strain.
  A.W.R. Hamad , M.A. Ibrahim , S.I. Al-Mohtasib and K.N. Al-Kobasi
  In the setting of neoplastic conditions, many biochemical substances deviate from their normal values; these may include proteins of body fluid. To verify this claim a prospective study was carried out to investigate the values of plasma proteins in salivary fluids in patients with primary brain tumors. This study had been conducted between September 2008 and July 2009 on 160 individuals. The qualified subjects for the study included, 80 patients (40 from each sex) with primary brain tumor and eighty age and sex normal subjects were used as control for salivary and serum proteins measurements. Concentrations of proteins were found higher in saliva and blood of male and female patients as compared with normal individuals. These results might suggest possible preliminary detection of primary brain tumor by observing the increase of protein concentration in the saliva of the patient which is easily carried out in clinical laboratory.
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