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Articles by M.A. Hassanain
Total Records ( 5 ) for M.A. Hassanain
  M.A. Hassanain , H.A. Elfadaly , R.M. Shaapan , N.A. Hassanain and A.M. Barakat
  Mutton signifies one of the most prevalent sources for human toxoplasmosis. However, sheep serological assays don't categorize the virulent strains initiating antibodies, so the biological bioassay of Egyptian mutton isolates with reference to their pathogenicity in both mice and kittens were done in this study for indicating to how extent their zoonotic bio-hazard. A total number of 280 of each sheep blood and tissue samples were collected during slaughtering at Cairo abattoir, Egypt. Sera assayed using Latex Agglutination Test (LAT) and immunosorbant assay (ELISA) and their corresponding mutton samples were microscopically examined after pepsin digestion for detection of Toxoplasma gondii infection. The sero-positive percent of the naturally infected sheep was 50.4 and 61.4 by LAT and ELISA, respectively, 47.9% of samples were confirmedly positive in both LAT and ELISA results. The microscopical examination revealed that only 28 out of 134 (20.9%) of the confirmed sero-positive animals by both tests were found harboring T. gondii tissue cysts in their mutton samples, while high percentage of confirmed sero-positve animals (79.1%) (106 out of 134) were biologically tissue cysts free mutton. Biological typing of the 28 T. gondii sheep isolates with reference to mice and kittens' bioassay indicated that 10.7, 50, 21.4 and 17.9% were type I, II, III and avirulent strains, respectively. The high T. gondii infection rate resulted in this study concludes that the feeding of under cooked mutton is a bad health habit as a source for human toxoplasmosis moreover; the T. gondii virulent strains obtained by mutton bioassay indicated that not all sero-positive sheep are connecting zoonotic bio-hazard through their mutton strains.
  M.A. Hassanain , Mona S. Mahmoud and Nawal A. Hassanain
  Serum samples collected from a total number of twenty parasitologically confirmed cases of human fasciolosis were used to evaluate the diagnostic sensitivity and specificity of snail derived antigens; non infected snail (SA), infected snail (ISA), redia (RA), cercaria (CA) and encysted metacercaria (EMCA) of F. gigantica using the enzyme linked immunosorbent assay (ELISA) and enzyme linked immunotransfer blot (EITB). ELISA results showed that the highest level of sensitivity (100%) was with the CA as compared with ISA, RA and EMCA which displayed lower sensitivity levels of 30, 60 and 90%, respectively. All fasciolosis patients were seronegative with SA. Sodium dodecyle sulfate polyacrylamide gel electrophoresis and EITB (with rabbit antiserum raised against F. gigantica somatic antigen) of the snail derived antigens were carried out to characterize their protein profiles and detect cross-reactive polypeptide bands between them. The immunoblot profile of SA displayed cross-reactive bands at 61 and 30 kDa with ISA, RA, CA and EMCA; CA at 61, 34 and 30 kDa with ISA; 96, 61, 34, 30 and 23 kDa with RA and 61, 34, 23 and 19 kDa with EMCA. Cross reactive antigens may be important as possible candidates for vaccine and diagnosis of fasciolosis. Immunoblot of sera from fasciolosis patients using fractionated cercarial antigen on nitrocellulose strips showed that all sera recognized common reactive band at 32.5 kDa molecular weight from cercarial antigen. We suggest that the 32.5 kDa component of Fasciola cercarial antigen may be the most sensitive and specific for the diagnosis of human fasciolosis.
  M.A. Hassanain , E.H. Abdel Rahman and F.A.M. Khalil
  The in vitro activity of three plant extracts; Artemisia cina, Peganum hormala and Calendula micrantha was investigated against Ascaridia galli adult worms. In vitro treatment of A. galli worms with different concentrations of each extract revealed potency of C. micrantha over the other two extracts as judged by the calculated LD50 values. These values were 2.66 ppm for C. micrantha, 33.7 ppm for P. harmala and 48.98 ppm for A. cina. The impact of the most potent extract, C. micrantha on A. galli worms was detected by scanning electron microscopy. C. micrantha treated worms showed enlargement and destruction of lips with subsequent damage of buccal cavity organelles. Moreover, papillae were disturbed and the amphids structures were lost. The cuticle showed wrinkled surface with loss of striations. Also, the cloacal structures were affected.
  R.M. Shaapan , M.A. Hassanain and Fathia A.M. Khalil
  Blood was collected from goats and buffaloes slaughtered for food in various areas of the Giza province, Egypt. A Modified Agglutination Test (MAT) with cut-off value 1:25 was used to test sera for evidence of exposure to Toxoplasma gondii. Serum antibody prevalence was higher 44.3% (102 positive of 230 tested) for goats than 22.5% (36 positive of 160 tested) for buffaloes. The antibody prevalence in relation to sex and age for each species was determined. Prevalence was higher (63.3 and 27.9%) in female than (32.1 and 16.2%) in male goats and buffaloes, respectively. On other hand, higher prevalence (58 and 25.5%) was detected in aged goats (>1.5 years) and buffaloes (>4 years) than (32.8 and 14.5%) detected in younger goats (≤1.5 years) and buffaloes (≤4 years), respectively. The present study is the first to report serological evidence of T. gondii infection in Egyptian goats and water buffaloes by MAT and determined the effect of sex and age. Consequently the finding results obtained scope the public health significance of goat and buffalo's meat and milk as source of human infection.
  M.A. Hassanain , Fathia A.M. Khalil , K.A. AbdEl-Razik and R.M. Shaapan
  Cryptosporidium parvum is a ubiquitous zonootic protozoan parasite which is associated with severe acute diarrhoea in humans and animals. Diarrhoiec fecal samples collected from young cattle calves at different localites in Behira Province, Egypt were coproscopically examined concerning the presence of Cryptosporidium spp., oocysts. Small sized Cryptosporidium oocysts were detected of typical shape and measurements of the C. parvum oocysts with a total prevalence of 54.4% (higher prevalence 61.6% in calves less than 1 month of age while lower 38.2% in calves aged 1-2 month). Molecular characterization was done using nested PCR amplification and partial sequence analysis. The nested PCR gave the expected 1st (1325 bp) and the 2nd (825 bp) PCR products from all the examined Egyptian isolates. The DNA sequence alignments and BLAST search analysis of the of Egyptian isolates of Cryptosporidium revealed 100% homology between the 825 bp amplified fragment of Egyptian isolates and the counterpart of the 18S rDNA sequences of C. parvum deposited in Gene bank and proved that the 10 positive PCR isolates were Cryptosporidium parvum. The high prevalence of Cryptosporidium parvum among calves obtained by this investigation has pointed to the existence of this zoonotic genotype and suggesting that there is a potential risk of Cryptosporidiosis as a zoonotic transmission between calves and humans in this region.
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