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Articles by M. Raftari
Total Records ( 2 ) for M. Raftari
  I. Pakzad , A. Rezaee , M.J. Rasaee , A.Z. Hosseini , B. Tabbaraee , S. Ghafurian , A.S. Abdulamir , F. Abu Bakar and M. Raftari
  Lipopolysaccharide (LPS) of Brucella abortus is an essential component for developing the subunit vaccine against brucellosis. B. abortus LPS was extracted by n-butanol, purified by ultracentrifugation and detoxified by alkaline treatment. Pyrogenicity and toxicity of B. abortus LPS and detoxified–LPS (D-LPS) were analyzed and compared with LPS of E. coli. Different groups of mice were immunized intraperitoneally with purified B. abortus LPS, D-LPS, a combination of LPS with human serum albumin (LPS-HSA) and B. abortus S19 bacteria; besides, control mice were inoculated with sterile saline. Two doses of vaccine were given 4 weeks apart. Mice were challenged intraperitoneally with virulent B. abortus 544 strain 4 weeks after the second dose of vaccine. Sera and spleens of mice were harvested 4 weeks after challenge. LPS-B. abortus was 10,000-fold less potent in LAL test and 100-fold less potent in eliciting fever in rabbits than in E. coli LPS. And D-LPS was very less potent in LAL test and eliciting fever in rabbits ordinary LPS. The antibody titer of anti-LPS immunoglobulin G (IgG) was higher than D-LPS. However, mice immunized with either LPS, D-LPS or LPS-HSA vaccines showed a significant protection against infection of the spleen (p<0.01). There was no significant difference between mice immunized with LPS and D-LPS in terms of protection (p<0.99). Therefore, it was concluded that D-LPS and LPS-HSA for B. abortus can be used as safer and more potent vaccines than ordinary LPS-B. abortus vaccine.
  M. Raftari , F. Azizi Jalilian , A.S. Abdulamir , R. Son , Z. Sekawy and A.B. Fatimah
  Researchers in the area of microbiological meat safety, in an attempt to reduce beef carcass contamination, try carcass-washing treatments as an effective method to control pathogenic bacteria. Spray wash treatments utilizing 3 concentrations (1, 1.5 and 2%) of acetic, lactic, propionic and formic acids were performed to evaluate their efficacy in reducing numbers of Escherichia coli O157: H7 on meat tissues at 4±1°C. The meat was decontaminated with hot water and then inoculated with E. coli O157: H7, which then was spray washed with organic acids for 15 sec separately. The population of E. coli O157: H7 significantly (p<0.05) reduced after being spray washed with all treatments. The lethality effect of all organic acids according to the concentration was 2% concentration >1.5% concentration >1% concentration. Mean log reductions of E. coli O157: H7showed that the antibacterial effect of formic acid >lactic acid >acetic acid >propionic acid. The results of this study also indicated that formic acid is a good antibacterial agent for decontaminating animals carcass surfaces.
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