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Articles by M. Omidi
Total Records ( 2 ) for M. Omidi
  M. Poorebrahim , A.H. Hoseinzadeh , M. Omidi , M. Asghari and N. Sanadgol
  Malaria, an infectious diseases, couses in almost 1 million deaths each year over the world. Artemisinin, a sesquiterpene lactone endoperoxide, is one of the most effective antimalarial drugs purified from Artemisia annua L. in China, 1970s. As the low content of this compound in plant many of studies have been focused on using elicitors affecting gene expression involved in artemisinin synthesis pathway. The main step to enhance artemisinin content in plant by using elicitors is characterization of key genes promoter. Cytochrome p450 Reductase (CPR) is one of the key enzymes that plays an important role in artemisinin synthesis pathway. Promoter sequence of key genes involving in artemisinin biosynthesis pathway included ADS, CYP71AV1 and DBR2 was isolated except CPRgene. We used standard Thermal Asymmetric Interlaced Polymerase Chain Reaction (TAIL-PCR) with some modification in thermal cycle numbers to isolate the unknown 5' flanking region of the CPRgene from Artemisia annua. Subsequent bioinformatics analysis to characterize functional cis-acting elements inside the promoter was performed. The 5' flanking sequence of CPR was cloned in pGEM-T Easy vector and sequenced. Subsequent sequence analysis for characterize functional motifs using bioinformatics software indicated a group of putative cis-acting elements such as TATA box, CAAT box, G box, W box and etc., inside the CPR promoter. This sequence was submitted in GenBank databases under the accession number KC243135. Present study demonstrated that characterization of cis-acting response elements can facilitate using elicitors to enhance artemisinin production in plant.
  R. Angoshtari , R. Tavakkol Afshari , S. Kalantari and M. Omidi
  The present study describes, firstly, the effect of cultivar, photoenvironment and different abscisic acid (ABA) treatments on somatic embryogenesis in Brassica napus and secondly, describes the effects of ABA and drying rates on acquisition desiccation tolerance in cultivar Opera. In embryogenesis experiment, 20 days old cotyledon-derived calli were cultured in somatic embryo induction media with different concentrations of ABA (0, 0.5, 10 and 50 μM). These were incubated either in the dark or light. The calli were transferred to the same medium with either the same ABA or different ABA concentrations every 10 days. After 30 days the numbers of mature somatic embryos were counted. The results showed that light stimulated somatic embryo formation and maturation. Furthermore, differences in ABA response related to somatic embryogenesis were observed between cultivars. Also, embryos were likely more sensitive to ABA in days 1-10 and 21-30. For desiccation experiment, three ABA treatments were chosen from cultivar Opera. Then these embryos were cultured in germination medium without dehydration or dehydrated either with a fast dehydration rate or a slow dehydration rate before culturing in germination medium. The results showed that ABA treated embryos were more desiccation tolerant than non ABA treated embryos. It also showed that as the concentration of ABA increased, embryos tolerance to desiccation also increased. Moreover, slow drying rate was more beneficial for desiccation tolerance induction than fast drying in ABA treated embryos. In addition, ABA treated embryos had higher germination rates even when they were not dehydrated.
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