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| Articles
by
M. Hashemitabar |
Total Records (
3 ) for
M. Hashemitabar |
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G. Saki
,
M. Hashemitabar
,
M. Abdollahi
and
S.H. Razie
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The aim of this study was to evaluate the effects of Vero cells on early embryonic cleavage rate and overcome cellblock of mice embryos in vitro. Female mice were super-ovulated by Intra-peritoneal injection of 5 IU Pregnant mare serum gonadotropine (PMSG) and 5 IU Human Chorionic Gonadotropine (HCG) 48 h later. The super-ovulated female NMRI mice placed individually with NMRI (Noda Medical Research Institute) males of proved fertility. The following morning, the females with positive vaginal plug were killed and cumulus-enclosed single cell embryos (2PN) were recovered. Two pronuclear (n=170) embryos were divided randomly into 2 groups: (1) co culture with vero cells (2) culture in simple culture medium. The rate of the development and the morphological appearance of mouse embryos in two groups were recorded daily for 120 h after retrieval in each system using inverted microscope. On day 5 of development the results showed that embryos cultured on vero cells had a significantly higher blastocyst and hatching formation rate than those in simple culture medium alone (p<0.05). It is concluded that Vero cells may improve mouse embryo development partly by increasing blastocyst formation, hatching blastocyst rate. |
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L.S. Khorsandi
,
M. Hashemitabar
,
M. Orazizadeh
and
N. Albughobeish
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The aim of this study is to investigate the effect of
Dex, a widely used GC, on apoptosis and expression of FasL protein in
the mouse testicular germ cells. The effects of dexamethasone (Dex) on
expression of Fas ligand (FasL), an important proapoptotic protein, in
the mouse testicular germ cells were investigated. Six groups, each of
8 male NMRI mice were chosen for the experiment. Experimental groups received
one of the following treatments daily for 7 days: 4, 7 and 10 mg kg-1
Dex. Control groups were treated with equivalent volumes of saline. Experimental
and control animals were sacrificed 24 h after the last injection. Immunohistochemical
procedure was used to evaluation of FasL expression and the deoxyuridine
nick-end labeling (TUNEL) was applied to assessment of the apoptotic germ
cells. FasL expression of testicular germ cells were significantly increased
in 10 mg kg-1 Dex treated mice (p<0.05), particularly at
stages VII-VIII of spermatogenic cycle. Apoptotic indexes (AIs) of germ
cells were significantly increased in 7 and 10 mg kg-1 Dex
treated mice. |
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M. Orazizadeh
,
M. Hashemitabar
,
M. Fakoor
and
M.T. Moghadam
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In this study, we investigated whether Bone Morphogenetic Protein-2 (BMP-2) could modulate dedifferentiation, apoptosis and proliferation capacity in the normal and OA cultured chondrocytes. The articular chondrocytes from normal (n = 4) and OA (n = 4) cartilages were harvested separately. The chondrocytes were cultured in monolayer in the presence of 100 ng mL-1 BMP-2 and 1% FBS as a test group and 1% FBS alone as a control group. Then, the chondrocytes were harvested and assessed for morphology with invert microscopy, proliferation by using MTT-assay and apoptosis with caspase-3 immunocytochemistry. The results indicated that the normal and the most OA chondrocytes showed round and polygonal appearance with chondrocyte-like morphology in BMP-2 treated groups after 6 days. The MTT proliferation test didn’t show significant difference between test and control groups. The OA cells showed proliferation rate higher than the normal cells and significant difference in the presence of BMP-2 was observed (p<0.05). Positive immunostaining of caspase-3 in test and control groups was 1 and 20% in normal and 30 and 43% in OA groups, respectively. The percentage of apoptosis was reduced in the presence of BMP-2. In conclusion, it appears that BMP-2 involves in suppression of dedifferentiation and apoptosis processes of cultured human chondrocytes. |
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