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Articles by M. Farsi
Total Records ( 8 ) for M. Farsi
  A. Jafari , M. Farsi , F. Soleimanian Shafa and N. Taghizadeh
  Salvia L. belongs to Lamiaceae family, have 900 species around the world. Seven of them distributed in Mashhad (North-East of Iran) which are following: S. spinosa L., S. staminae Montbr and Auch, S. sclarea L., S. chloroleuca Rech and Aell., S. virgata Jacq, S. chorassanica Bung. and S. nemorosa L. In present research seed proteins of Salvia sclarea L., S. spinosa L. and S. chloroleuca Rech and Aell. studied by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. The aim of present study was identifying the variation between seed protein in populations and relationship with morphological characters and geographical distance. Analysis showed 22 bands from which some of them were specific for a population. Cluster analysis of populations were carried out. The variation bands have correspondence to geographical distance and morphological characters.
  E. Valiellahi , A. Niazi and M. Farsi
  The objective of this study was to determine the quantitative expression of COR gene (Wcor14) in different cold treatment in Iranian winter-type wheat, using semiquantitative RT-PCR analysis. Semiquantitative RT-PCR analysis showed that Wcor14 specifically induced by low temperature. The transcripts of Wcor14 were up-regulated within 3-6 h of cold acclimation at 4°C.
  E. Valiellahi , A. Niazi and M. Farsi
  To determine the genetic nature of these mechanisms, several cold-responsive genes were identified. Wcor14, a member of the wheat cold-responsive (Cor) gene family, in this study has been isolated and characterized from ancient wheat ancestor Aegilops tauschii. The deduced polypeptide Aegilops tauschii WCOR14 is a hydrophobic polypeptide with 140 amino acids (MW = 13.5 kDa) showed high homology to the previously identified wheat and barley COR proteins. Analyses of the cDNA and genomic DNA sequences in this study suggested that, Aegilops tauschii Wcor14 and its related sequences constitute a small multigene family with different intron sizes. The transcripts Analyses of Wcor14 suggested that Wcor14 transcripts were up-regulated within 3-6 h of cold acclimation at 4°C. We used SCRATCH server for protein structure prediction.
  A. Mirshamsi , M. Farsi , F. Shahriari and H. Nemati
  Random Amplified Polymorphic DNAs (RAPD) were used to estimate genetic distances and determine the correlation between genetic distance and hybrid performance of 29 tomato lines that were the parents in a diallel mating design. Among 97 observed bands, 69 showed polymorphism and were used for establishing genetic distances based on the Nei coefficient between parents. A UPGMA dendrogram and Multi-Dimensional Scaling (MDS) analysis based on Nei genetic distances clearly clustered each group, confirming the variation at a molecular level. Correlations between genetic distances of the parents and performances of hybrids were established for various quantitative traits. Significant correlations were found between RAPD markers estimated genetic distances and MPH, HPH, SCA for some traits. The low correlation between parental genetic distances and hybrid performances for some quantitative traits suggested that RAPD markers have low linkage to Quantitative Trait Loci (QTLs) or have inadequate genome coverage for these traits. The results indicated that RAPD markers can be used as a tool for determining the extent of genetic diversity among tomato lines, for allocating genotypes into different groups and also to aid in the choice of the superior crosses to be made among tomato lines, so reducing the number of crosses required under field evaluation.
  H.R. Kavousi , M. Farsi and F. Shahriari
  The secondarily homothallic life cycle of the white button mushroom that results in scarcity of uninucleate basidiospores (homokaryons) in its progeny, is the most important impediment for genetic improvement of the commercial strains. Identification of homokaryons for breeding programs of Agaricus bisporus (button mushroom) is, therefore, crucial. Verifying homokaryons through fruiting trial is time consuming and unreliable. In this study, ability of RAPD markers, compared to morphological characters for identification of homokaryon isolates, was investigated. Based on morphological characters, 42 isolates were screened and exposed to RAPD markers. The results showed that RAPD markers could discriminate homokaryons from heterokaryons, based on number of bands generated. The numbers of band in homokaryons were significantly less than those of heterokaryons. Results also showed that cluster analysis, based on average of band number generated, could separate homokaryon from heterokaryon isolates. It is suggested that RAPDs could be used to identify hyomokaryons from heterokaryons for breeding program of A. bisporus.
  E.D. Uliaie , M. Farsi , B. Ghreyazie and J. Imani
  Ability of shoot regeneration from cotyledon explants of rapeseed (Brassica napus) winter genotypes and the effect of silver nitrate and naphthalene acetic acid in shoot regeneration were investigated. The optimum medium for regeneration was the medium supplemented with 3 mg L-1 6-Benzylaminopurine and 0.15 mg L-1 1-naphthaleneacetic acid. The addition of 5 mg L-1 silver nitrate significantly improved shoot regeneration. Shoot regeneration response was strongly different between genotypes with a range of variation from 79% in spring genotype PF and 7% in Okpi. The highest root production was recorded on medium containing indol-3-butyric acid. The rooted plants successfully transferred to soil and adapted to greenhouse conditions. No abnormally was observed and the regenerated plants were morphologically similar to the field grown parental plants.
  E. Fazeli , F. Shahriari , H. Samizadeh , A. Bagheri and M. Farsi
  In current research, genetic relationships among rapeseed genotypes from several geographical origins including France, Canada, Germany, Iran, Hungary, Denmark, Australia and America were evaluated using RAPD markers. Among generated 86 bands, 80 different polymorphic bands were obtained using 9 random primers. Diversity Index (DI) or Polymorphism Information Content (PIC) was varied from 0.29 to 0.48, showed a relatively high potential of primers among studied genotypes. Dice similarity coefficient between genotypes was calculated using Nei and Li formula. Maximum (0.91) and minimum (0.42) similarity coefficients were observed between Bristol and Amber genotypes, consul and express, respectively. Cluster analysis based on dice similarity coefficient was also carried out. Base on the cluster analysis, genotypes were grouped into five main clusters. Results showed that genotypes with same geographical origin were genetically different. Therefore, geographical origins of genotypes cannot be used as a base to cross parent to obtain high heterosis and it must be carried out by exact genetic studies. Results confirmed that RAPD is a simple, cheap and fast method for evaluation of genetic diversity of Brassica napus.
  F. Shahriari-Ahmadi , E. Dehghan , M. Farsi and M. Azizi
  In this study the tip of meristem in diploid plants (2n = 2x =28) were treated with a 0.2% colchicine solution and flowcytometry was used to determine the DNA ploidy level of different tissues in individual plants. Morphological characteristics such as size and thickness of leaves, flowers, seeds, epidermis and guard cells, as well as the number of chloroplast in guard cells were considered as other parameters for identifying putative tetraploid plants. We show that, the DNA ploidy of Hyoscyamus muticus, treated with Colchicine can be evaluated using flow cytometry. In addition we also report reliable morphological markers to identify putative tetraploids in this species. Although, tetraploidy was induced using this technique, mixoploid plants were also observed among the Egyptian henbane plants. The results of this study also indicate that colchicine treatment can efficiently be used to improve Hyoscyamus muticus for commercial and pharmaceutical applications of its vegetative organs.
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