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Articles by M. Anisuzzaman
Total Records ( 3 ) for M. Anisuzzaman
  M. Anisuzzaman , S. Jarin , K. Naher , M.M. Akhtar , M.J. Alam , M. Khalekuzzaman , I. Alam and M.F. Alam
  A viable protocol has been developed for indirect shoot organogenesis of okra. To establish a stable and high-frequency plant regeneration system, leaf disc and hypocotyl explants were tested with different combinations of α-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA),iIndole-3-butyric acid (IBA), thidiazuron (TDZ) and 6-benzylaminopurine (BAP). Morphogenic callus induction was observed in highest frequency from hypocotyl explant by culturing in MS medium supplemented with 2.0 mg L-1 NAA plus 0.5 mg L-1 TDZ. The highest percentage of shoot regeneration and highest mean number of shoot per callus mass was obtained with 2.0 mg L-1 BAP plus 0.1 mg L-1 IBA. Root formation was observed from callus induced in medium containing 1.5 mg L-1 NAA. Morphogenic difference due to explant type is clear for the studied in vitro traits. About 80% of regenerated plantlets were survived and showed new leaves development under ex vitro condition. This protocol would be useful to create somaclonal variation and to utilize transgenic approach for varietal improvement of okra.
  M. Anisuzzaman , S.A. Sharmin , S.C. Mondal , R. Sultana , M. Khalekuzzaman , I. Alam and M.F. Alam
  In vitro microrhizome was successfully produced in Curcuma zedoaria-a valuable but critically threatened medicinal plant. Ten to twelve weeks old in vitro multiplied shoots were cultured in MS medium supplemented with different level of BAP and NAA. Effect of different carbon sources has also been evaluated. Microrhizome formation started within 7-9 weeks of culture. The best result was obtained in 4.0 mg L-1 BAP along with 6% sucrose in terms of frequency and number of rhizome. Around 4 microrhizomes with an average weight of 2.5 g were harvested after 11-12 weeks. Microrhizomes were readily germinated in the growth regulator free MS medium. With the best of our knowledge this is the first report on in vitro microrhizome production in Curcuma zedoaria.
  N.A. Siddique , Jaime A. Teixeira da Silva , M. Anisuzzaman and M.A. Bari
  A procedure for rapid in vitro propagation of the aromatic and medicinal plants Aristolochia indica Linn and Hemidesmus indicus R.B (Family: Aristolochiaceae and Asclepiadaceae respectively) from axillary shoots is described. The highest percentage of callus induction was 92% on MS medium supplemented with 1.0 mg L-1 α-naphthalene acetic acid (NAA) and 1.0 mg L-1 6-benzyladenine (BA) for A. indica while 98% was achieved on MS medium supplemented with 0.5 mg L-1 NAA and 2.0 mg L-1 Kinetin (Kn) for H. indicus. The colour of the calli was mostly light to dark green. Development of adventitious shoots occurred when calli were subcultured on MS medium supplemented with BA and Kn alone or in BA combined with NAA and IAA, or NAA, IAA and BAP combined with Kn. The highest percentage (90%) of shoot regeneration in A. indica was obtained on MS medium fortified with 1.0 mg L-1 BA and 2.5 mg L-1 NAA, but a 98% rate on MS medium supplemented with 2.5 mg L-1 Kn and 1.0 mg L-1 NAA for H. indicus. Regenerated shoots rooted best on MS medium containing 2.5 mg L-1 Kn and 2.0 mg L-1 IBA. Plantlets were transferred to pots containing sand and soil mixture and acclimatized in a culture room. Finally rooted plants were transferred to soil.
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